Physiology

Effects of Arginine Vasopressin on Islet Cells in Pancreatic Tissue Slices: Glucose-Dependent Modulation of IP3 Receptor-Specific Responses

1. Jasmina Kerčmar
2. Nastja Murko
3. Lidija Križančić Bombek
4. Eva Paradiž Leitgeb
5. Johannes Pfabe
6. Sandra Postić
7. Ya-Chi Huang
8. Andraž Stožer
9. Dean Korošak
10. Xaver Kozisek
11. Monika Perisic
12. Markus Muttenthaler
13. Christian W Gruber
14. Marjan Slak Rupnik

• Curated by eLife

  eLife Assessment

  This study presents a useful finding on the effects of arginine vasopressin (AVP) on islet cells in pancreatic tissue slices, using technically sophisticated spatio-temporal calcium recordings to confirm that AVP influences α and β cells differently depending on glucose concentrations. While the study’s methods – particularly the calcium imaging techniques and peptide ligand design targeting V1b receptors – are strong, the reviewers were concerned about several aspects of the experimental design. The results on β-cell responses are incomplete and insufficient to support the manuscript’s claims, especially due to the high variability of islet responses and lack of mechanistic and functional (hormone release) data. There are also concerns about the possibility of off-target effects and suboptimal receptor specificity: the study would be significantly strengthened by inclusion of signaling pathway interrogation, hormone output assays, genetic validation (e.g., β cell-specific deletion of V1br), and receptor localization. The work will still be of interest to researchers studying islet physiology in the context of health and diabetes.

Reviewed by eLife

Sex-dependent effects of a gestational ketogenic diet on offspring birth and lifespan

1. Sarah M. Zala
2. Renata Santos
3. Eva Strasser
4. Alice Schadde
5. Sarah Kugler
6. Verena Strauss
7. Anna Kübber-Heiss
8. Diana Zala

Reviewed by PREreview

Iron-deplete diet enhances Caenorhabditis elegans lifespan via oxidative stress response pathways

1. Priyanka Das
2. Ravi
3. Jogender Singh

Reviewed by Review Commons

Endurance Exercise Ameliorates Aging-Related Bradyarrhythmia in Drosophila Resulting from miR-283 Knockdown in LNvs

1. Qiufang Li
2. Xu Ping
3. Zhengwen Yu
4. Qin Yi
5. Chao Tang
6. Xiaoya Wang
7. Lan Zheng

• Curated by eLife

  eLife Assessment

  This study offers valuable insights into the role of miR-283 in ventral-lateral neurons (LNvs) and its impact on senescence, cardiac function, and aging in the Drosophila melanogaster model. However, the evidence supporting some of the conclusions remains incomplete, and further mechanistic studies are needed to clarify how miR-283 affects normal aging and influences exercise adaptations. Nonetheless, the work can be of interest to cell biologists studying miRNA biology, aging, and age-related diseases.

Reviewed by eLife

Zinc is a Key Regulator of the Sperm-Specific K+ Channel (Slo3) Function

1. Rizki Tsari Andriani
2. Tanadet Pipatpolkai
3. Haruhiko Miyata
4. Masahito Ikawa
5. Yasushi Okamura
6. Takafumi Kawai

• Curated by eLife

  eLife Assessment

  This study presents a useful contribution to understanding zinc regulation of sperm physiology, specifically its inhibitory effects on the sperm-specific potassium channel Slo3. However, the evidence supporting the claims is incomplete, as critical experimental controls are lacking, key mechanistic aspects remain insufficiently explored, and experimental descriptions are often inadequate, making it difficult to fully assess the findings. Strengthening the study with additional electrophysiological recordings in sperm cells, improved imaging controls, and clearer methodological descriptions would enhance its impact and rigor.

Reviewed by eLife

Peripheral opioid receptor antagonism alleviates fentanyl-induced cardiorespiratory depression and is devoid of aversive behavior

1. Brian C Ruyle
2. Sarah Masud
3. Rohith Kesaraju
4. Mubariz Tahirkheli
5. Juhi Modh
6. Caroline G Roth
7. Sofia Angulo-Lopera
8. Tania Lintz
9. Jessica A Higginbotham
10. Nicolas Massaly
11. Jose A Morón

• Curated by eLife

  eLife Assessment

  This manuscript represents a fundamental contribution demonstrating that fentanyl-induced respiratory depression can be reversed with a peripherally-restricted mu opioid receptor antagonist. The paper reports compelling and rigorous physiological, pharmacokinetic, and behavioral evidence supporting this major claim, and furthers mechanistic understanding of how peripheral opioid receptors contribute to respiratory depression. These findings reshape our understanding of opioid-related effects on respiration and have significant therapeutic implications given that medications currently used to reverse opioid overdose (such as naloxone) produce severe aversive and withdrawal effects via actions within the central nervous system.

Reviewed by eLife

Loss of CTRP10 results in female obesity with preserved metabolic health

1. Fangluo Chen
2. Dylan C Sarver
3. Muzna Saqib
4. Leandro M Velez
5. Susan Aja
6. Marcus M Seldin
7. G William Wong

• Curated by eLife

  eLife Assessment

  This manuscript presents a detailed characterization of male and female wildtype and Ctrp10 knockout mice, and reveals that knockout mice develop female-specific obesity that is largely uncoupled from metabolic dysfunction. The data are convincing, and the work will be an important contribution to understanding how obesity is coupled to metabolic dysfunction, and how this can occur in a sex-specific manner.

Reviewed by eLife

Adiponectin exerts sex-dependent effects on lipid, amino acid, and glucose metabolism during caloric restriction

1. Yoshiko M Ikushima
2. Kuan-Chan Chen
3. Richard J. Sulston
4. Domenico Mattiucci
5. Eleanor J. Brain
6. Stefanie A Fung Xin Zi
7. Karla J. Suchacki
8. Benjamin J. Thomas
9. Andrea Lovdel
10. Matthew Bennett
11. Hiroshi Kobayashi
12. Phillip D. Whitfield
13. Keiyo Takubo
14. Andrew H. Baker
15. Nicholas M. Morton
16. Robert K. Semple
17. William P. Cawthorn

Reviewed by PREreview

The Kv2.2 channel mediates the inhibition of prostaglandin E2 on glucose-stimulated insulin secretion in pancreatic β-cells

1. Chengfang Pan
2. Ying Liu
3. Liangya Wang
4. Wen-Yong Fan
5. Yunzhi Ni
6. Xuefeng Zhang
7. Di Wu
8. Chenyang Li
9. Jin Li
10. Zhaoyang Li
11. Rui Liu
12. Changlong Hu

• Curated by eLife

  eLife Assessment

  The study presents valuable findings on the molecular mechanisms of glucose-stimulated insulin secretion from pancreatic islets, focusing on the main regulatory elements of the signaling pathway in physiological conditions. While the evidence supporting the conclusions is solid, the study can be strengthened by the use of a beta cell line or knockout mice. The work will be of interest to cell biologists and biochemists working on diabetes.

Reviewed by eLife

Multi-dimensional oscillatory activity of mouse GnRH neurons in vivo

1. Su Young Han
2. Shel-Hwa Yeo
3. Jae-Chang Kim
4. Ziyue Zhou
5. Allan E Herbison

• Curated by eLife

  eLife Assessment

  This valuable study investigates the oscillatory activity of gonadotropin-releasing hormone (GnRH) neurones in mice using GCaMP fiber photometry. It demonstrates three distinct patterns of oscillatory activity that occur in GnRH neurons comprising low-level rapid baseline activity, abrupt short-duration oscillations that drive pulsatile gonadotropin secretion, and, in females, a gradual and prolonged oscillating increase in activity responsible for the relatively short-lived preovulatory LH surge. The evidence presented in the study is solid, offering theoretical implications for understanding the behaviour of GnRH neurones in the context of reproductive physiology, and will be of interest to researchers in neuroendocrinology and reproductive biology.

Reviewed by eLife