Physiology

Mechano-regulation of GLP-1 production by Piezo1 in intestinal L cells

1. Yanling Huang
2. Haocong Mo
3. Jie Yang
4. Luyang Gao
5. Tian Tao
6. Qing Shu
7. Wenying Guo
8. Yawen Zhao
9. Jingya Lyu
10. Qimeng Wang
11. Jinghui Guo
12. Hening Zhai
13. Linyan Zhu
14. Hui Chen
15. Geyang Xu

• Curated by eLife

  eLife Assessment

  This study focuses on the regulation of GLP-1 in enteroendocrine L cells and how this may be stimulated by the mechanogated ion channel Piezo1 and the CaMKKbeta-CaMKIV-mTORC1 signaling pathway. The work is innovative and is considered valuable, as the hypothesis that is being tested may have significant mechanistic and translational implications. Data to support the proposed mechanism were considered incomplete, yet data to support the overall physiological characterization were considered solid.

Reviewed by eLife

Calcineurin inhibition enhances Caenorhabditis elegans lifespan by defecation defects-mediated calorie restriction and nuclear hormone signaling

1. Priyanka Das
2. Alejandro Aballay
3. Jogender Singh

• Curated by eLife

  eLife Assessment

  This important study reveals insights into how calcineurin influences C. elegans pathogen susceptibility and lifespan through its role in controlling the defecation motor program. The authors provide convincing evidence to support a new mechanism through which calcineurin impacts longevity. This work will be of interest to investigators studying host-pathogen interactions and aging in a number of experimental systems.

Reviewed by eLife

Exceptional longevity of mammalian ovarian and oocyte macromolecules throughout the reproductive lifespan

1. Ewa K Bomba-Warczak
2. Karen M Velez
3. Luhan T Zhou
4. Christelle Guillermier
5. Seby Edassery
6. Matthew L Steinhauser
7. Jeffrey N Savas
8. Francesca E Duncan

• Curated by eLife

  eLife assessment

  This important study highlights cell types preserving long-lived proteins and lays a foundation for identifying exceptionally long-lived proteins in the ovary. Convincing evidence describes helpful data about protein turnover and identifies long-lived macromolecules in oocytes and somatic cells during mouse ovarian aging. This work will be of interest to researchers working on aging and reproductive health.

Reviewed by eLife

Sympathetic motor neuron dysfunction is a missing link in age-associated sympathetic overactivity

1. Lizbeth de La Cruz
2. Derek Bui
3. Claudia M Moreno
4. Oscar Vivas

• Curated by eLife

  eLife assessment

  This important study describes changes in excitability in motor neurons of the peripheral autonomous nervous system during aging. The manuscript provides convincing evidence indicating that sympathetic neurons from aged mice show higher excitability compared to neurons from young mice which was linked to decreased activity of KCNQ2/3 potassium channels. This research has implications for understanding the age-related changes that occur in the peripheral nervous system.

Reviewed by eLife

Dysregulated Ca2+ signaling, fluid secretion, and mitochondrial function in a mouse model of early Sjögren’s disease

1. Kai-Ting Huang
2. Larry E Wagner
3. Takahiro Takano
4. Xiao-Xuan Lin
5. Harini Bagavant
6. Umesh Deshmukh
7. David I Yule

• Curated by eLife

  eLife assessment

  This manuscript presents important observations on the early changes that occur in calcium signaling, TMEM16a channel activation, and mitochondrial dysfunction in salivary gland cells in a murine model of autoimmune Sjögren's disease. The study reports that in response to DMXAA treatment which induces a murine model of Sjögren's disease, salivary gland cells show significant changes in saliva release, calcium signaling, TMEM16a activation, mitochondrial function, and sub-cellular morphology of the endoplasmic reticulum. The work is compelling and will be of strong interest to physiologists working on secretion, calcium signaling, and mitochondria.

Reviewed by eLife

A kidney-hypothalamus axis promotes compensatory glucose production in response to glycosuria

1. Tumininu S Faniyan
2. Xinyi Zhang
3. Donald A Morgan
4. Jorge Robles
5. Siresha Bathina
6. Paul S Brookes
7. Kamal Rahmouni
8. Rachel J Perry
9. Kavaljit H Chhabra

• Curated by eLife

  eLife assessment

  The study presents valuable findings on compensatory mechanisms in response to glycosuria. The evidence supporting the claims is solid, although a causal relationship is somewhat uncertain and the addition of a more clinically relevant model would have strengthened the findings. The work will be of interest to diabetes investigators.

Reviewed by eLife, PREreview

An acute microglial metabolic response controls metabolism and improves memory

1. Anne Drougard
2. Eric H Ma
3. Vanessa Wegert
4. Ryan Sheldon
5. Ilaria Panzeri
6. Naman Vatsa
7. Stefanos Apostle
8. Luca Fagnocchi
9. Judith Schaf
10. Klaus Gossens
11. Josephine Völker
12. Shengru Pang
13. Anna Bremser
14. Erez Dror
15. Francesca Giacona
16. Sagar Sagar
17. Michael X Henderson
18. Marco Prinz
19. Russell G Jones
20. John Andrew Pospisilik

• Curated by eLife

  eLife assessment

  This important study demonstrates a link between an acute high fat diet, microglial metabolism and improved higher cognitive function. The evidence supporting the proposed mechanism in vivo is incomplete at this stage due to non-trivial technical limitations but the authors provide convincing in vitro metabolic characterization of primary microglia cultures to support the model. This work will be of interest to a broad audience in the field of neuroscience, metabolism, and immunology.

Reviewed by eLife

Genetic code expansion, click chemistry, and light-activated PI3K reveal details of membrane protein trafficking downstream of receptor tyrosine kinases

1. Duk-Su Koh
2. Anastasiia Stratiievska
3. Subhashis Jana
4. Shauna C Otto
5. Teresa M Swanson
6. Anthony Nhim
7. Sara Carlson
8. Marium Raza
9. Ligia Araujo Naves
10. Eric N Senning
11. Ryan A Mehl
12. Sharona E Gordon

• Curated by eLife

  eLife assessment

  This study develops a new and important method for dissecting out two overlapping cell signaling pathways, phosphoinositide signaling and membrane protein trafficking. The combination of two state-of-the-art spectroscopic techniques provides compelling evidence for a reciprocal influence between an enzyme and a channel. The work will be of interest to the broader cell biology, biophysics and biochemistry communities.

Reviewed by eLife

Reliance on self-reports and estimated food composition data in nutrition research introduces significant bias that can only be addressed with biomarkers

1. Javier I Ottaviani
2. Virag Sagi-Kiss
3. Hagen Schroeter
4. Gunter GC Kuhnle

• Curated by eLife

  eLife assessment

  This important study, using three bioactive compounds as a model, demonstrates that estimating the intake of food components based on food composition databases and self-reported dietary data is highly unreliable. The authors present convincing data showing the differences in the estimated quantile of intake of three bioactive compounds between biomarker and 24-hour dietary recall with food-composition database. The work will be of broad interest to the clinical nutrition research community.

Reviewed by eLife

ATP and glutamate coordinate contractions in the freshwater sponge Ephydatia muelleri

1. Vanessa R Ho
2. Greg G Goss
3. Sally P Leys

Reviewed by Arcadia Science