Physiology

Blood pressure variability compromises vascular function in middle-aged mice

1. Perenkita J Mendiola
2. Philip O'Herron
3. Kun Xie
4. Michael W Brands
5. Weston Bush
6. Rachel E Patterson
7. Valeria Di Stefano
8. Jessica A Filosa

• Curated by eLife

  eLife Assessment

  This is an important study that demonstrates that blood pressure variability impairs myogenic tone and diminishes baroreceptor reflex. The study also provides evidence that blood pressure variability blunts functional hyperemia and contributes to cognitive decline. The evidence is compelling whereby the authors use appropriate and validated methodology in line with or more rigorous than the current state-of-the-art.

Reviewed by eLife

The hypoxic response extends lifespan through a bioaminergic and peptidergic neural circuit

1. Elizabeth S Kitto
2. Shijiao Huang
3. Mira Bhandari
4. Cassie Tian
5. Rebecca L Cox
6. Safa Beydoun
7. Emily Wang
8. Danielle Shave
9. Hillary A Miller
10. Sarah A Easow
11. Ella Henry
12. Megan L Schaller
13. Scott F Leiser

• Curated by eLife

  eLife Assessment

  This fundamental study identifies specific neural mechanisms through which HIF-1 signaling in ADF serotonergic neurons extends lifespan in C. elegans, revealing that downstream signaling in multiple types of neurons, as well as other neuromodulators like GABA, tyramine, and NLP-17, is required for this effect. The strength of the evidence is largely convincing, as the authors establish the necessity and causality of key neuronal components using multiple genetic tools and functional dissection in a well-validated model organism.

Reviewed by eLife

Loss of the alternative calcineurin variant CnAβ1 enhances brown adipocyte differentiation and drives metabolic overactivation through FoxO1 activation

1. Elísabet Bello-Arroyo
2. Blanca Rubio
3. Alfonso Mora
4. Marina López-Olañeta
5. Jesús M. Gómez-Salinero
6. Laura Ramos
7. Carlos G. Sánchez-Cabezudo
8. Emilio Camafeita
9. Lorena Cusso
10. Manuel Desco
11. Nolwenn Joffin
12. Johanne Le Coq
13. Jasminka Boskovic
14. Kathryn A. McGurk
15. James S. Ware
16. Paul J. R. Barton
17. Jesús Vázquez
18. Philipp E. Scherer
19. Guadalupe Sabio
20. María Victoria Gómez-Gaviro
21. Enrique Lara-Pezzi

Reviewed by Review Commons

Transient hypoxia followed by progressive reoxygenation is required for efficient skeletal muscle repair through Rev-ERBα modulation

1. Marie Quétin
2. Audrey Der Vartanian
3. Christelle Dubois
4. Juliette Berthier
5. Marine Ledoux
6. Stéphanie Michineau
7. Bernadette Drayton-Libotte
8. Athanassia Sotiropoulos
9. Frédéric Relaix
10. Marianne Gervais

Reviewed by Review Commons

Cellular characterization of the mouse collecting lymphatic vessels reveals that lymphatic muscle cells are the innate pacemaker cells

1. Scott D Zawieja
2. Grace A Pea
3. Sarah E Broyhill
4. Advaya Patro
5. Karen H Bromert
6. Charles E Norton
7. Hae Jin Kim
8. Sathesh Kumar Sivasankaran
9. Min Li
10. Jorge A Castorena-Gonzalez
11. Bernard T Drumm
12. Michael J Davis

• Curated by eLife

  eLife Assessment

  This manuscript aims to identify the pacemaker cells in the lymphatic collecting vessels - the cells that initiate the autonomous action potentials and contractions needed to drive lymphatic pumping. Through the exemplary use of existing approaches (genetic deletions and cytosolic calcium detection in multiple cell types), the authors convincingly determine that lymphatic muscle cells are the origin of the action potential that triggers lymphatic contraction. The inclusion of scRNAseq and membrane potential data enhances a tremendous study. This fundamental discovery establishes a new standard for the field of lymphatic physiology.

Reviewed by eLife

Cell-autonomous and non-cell-autonomous effects of Arginase 2 on cardiac aging

1. Duilio M Potenza
2. Xin Cheng
3. Guillaume Ajalbert
4. Andrea Brenna
5. Marie-Noelle Giraud
6. Aurelien Frobert
7. Stephane Cook
8. Kirsten D Mertz
9. Zhihong Yang
10. Xiu-Fen Ming

• Curated by eLife

  eLife Assessment

  This study provides fundamental information on how Arg-II participates in cardiac aging. The phenotypic data provide convincing evidence of non-cell-autonomous contributions to aging-related pathologies. Overall, the study highlights the importance of intercellular signaling in maintaining cardiac health during aging.

Reviewed by eLife

TRPV4 activation by TGFβ2 enhances cellular contractility and drives ocular hypertension

1. Christopher Nass Rudzitis
2. Monika Lakk
3. Ayushi Singh
4. Sarah N Redmon
5. Denisa Kirdajová
6. Yun-Ting Tseng
7. Michael L De Ieso
8. W Daniel Stamer
9. Samuel Herberg
10. David Krizaj

• Curated by eLife

  eLife Assessment

  This fundamental work extends our understanding of the role of TGFβ2 as a modulator of mechanosensing in the eye and identifies the TRPV4 ion channel as a common regulator of Trabecular Meshwork (TM) contractility and pathological OHT and the data and evidence provided are convincing. This work will clearly be of interest to researchers investigating the role of mechanosensors in the TM and may underpin future research into treatments that aim to lower intra ocular pressure. This work will additionally be of interest to the growing field of researchers investigating the regulation of force sensing via ion channels and their roles in health and disease, in particular the ion channel TRPV4.

Reviewed by eLife

Effects of Arginine Vasopressin on Islet Cells in Pancreatic Tissue Slices: Glucose-Dependent Modulation of IP3 Receptor-Specific Responses

1. Jasmina Kerčmar
2. Nastja Murko
3. Lidija Križančić Bombek
4. Eva Paradiž Leitgeb
5. Johannes Pfabe
6. Sandra Postić
7. Ya-Chi Huang
8. Andraž Stožer
9. Dean Korošak
10. Xaver Kozisek
11. Monika Perisic
12. Markus Muttenthaler
13. Christian W Gruber
14. Marjan Slak Rupnik

• Curated by eLife

  eLife Assessment

  This study presents a useful finding on the effects of arginine vasopressin (AVP) on islet cells in pancreatic tissue slices, using technically sophisticated spatio-temporal calcium recordings to confirm that AVP influences α and β cells differently depending on glucose concentrations. While the study’s methods – particularly the calcium imaging techniques and peptide ligand design targeting V1b receptors – are strong, the reviewers were concerned about several aspects of the experimental design. The results on β-cell responses are incomplete and insufficient to support the manuscript’s claims, especially due to the high variability of islet responses and lack of mechanistic and functional (hormone release) data. There are also concerns about the possibility of off-target effects and suboptimal receptor specificity: the study would be significantly strengthened by inclusion of signaling pathway interrogation, hormone output assays, genetic validation (e.g., β cell-specific deletion of V1br), and receptor localization. The work will still be of interest to researchers studying islet physiology in the context of health and diabetes.

Reviewed by eLife

Sex-dependent effects of a gestational ketogenic diet on offspring birth and lifespan

1. Sarah M. Zala
2. Renata Santos
3. Eva Strasser
4. Alice Schadde
5. Sarah Kugler
6. Verena Strauss
7. Anna Kübber-Heiss
8. Diana Zala

Reviewed by PREreview

Iron-deplete diet enhances Caenorhabditis elegans lifespan via oxidative stress response pathways

1. Priyanka Das
2. Ravi
3. Jogender Singh

Reviewed by Review Commons