Physiology

Efficient transduction of pancreas tissue slices with genetically encoded calcium integrators

1. Charles S Lazimi
2. Austin E Stis
3. Julia K Panzer
4. Helmut Hiller
5. Maria L Beery
6. Amelia K Linnemann
7. Cherie L Stabler
8. Clayton E Mathews
9. Edward A Phelps

• Curated by eLife

  eLife Assessment

  This study presents a valuable advance by enabling functional mapping of Ca²⁺ responses in live human pancreatic tissue slices, providing new opportunities to study islet heterogeneity and diabetes-related dysfunction in an intact tissue context. The evidence supporting the main conclusions is solid, based on reproducible methodology and functional validation across multiple human donor samples. Key revisions needed include clearer quantification of transduction efficiency and tissue viability, and improved clarification of how CaMPARI2 signals should be interpreted.

Reviewed by eLife

PIEZO channels link mechanical forces to uterine contractions in parturition

1. Yunxiao Zhang
2. Sejal A. Kini
3. Sassan A. Mishkanian
4. Renhao Luo
5. Saba Heydari Seradj
6. Verina H. Leung
7. Yu Wang
8. M. Rocío Servín-Vences
9. William T. Keenan
10. Utku Sonmez
11. Oleg Yarishkin
12. Manuel Sanchez-Alavez
13. Yuejia Liu
14. Xin Jin
15. Darren J. Lipomi
16. Li Ye
17. Michael Petrascheck
18. Antonina I. Frolova
19. Sarah K. England
20. Ardem Patapoutian

Reviewed by PREreview

NAD+ boosting by oral nicotinamide mononucleotide administration regulates key metabolic and immune pathways through SIRT1 dependent and independent mechanisms to mitigate diet-induced obesity and dyslipidemia in mice

1. Yasser Majeed
2. Najeeb M Halabi
3. Rudolf Engelke
4. Hina Sarwath
5. Muna N Al-Noubi
6. Sunkyu Choi
7. Aisha Al-Malki
8. Maha V Agha
9. Muneera Vakayil
10. Lotfi Chouchane
11. Frank Schmidt
12. Nayef A Mazloum

• Curated by eLife

  eLife Assessment

  The mechanistic basis for the potential health benefits of NAD⁺ precursors remains incompletely understood. This manuscript provides a useful assessment of the role of SIRT1 in mediating the effects of NMN in mice fed a high-fat diet. The study addresses a key question, though some of the conclusions appear only partially supported by the presented data.

Reviewed by eLife

E4BP4 Safeguards Brown Fat Mitochondria from Obesity-Induced Fragmentation via Ceramide Repression

1. Fernando Valdivieso-Rivera
2. Vanessa O. Furino
3. Carlos E. Leher
4. Ariane M. Zanesco
5. Monara Kaélle Cruz
6. Flavia C. Gan
7. Adriana Leandra Santoro
8. Lara Regina-Ferreira
9. Giovanna Leite Santos
10. Tiago Gonçalves
11. Luiz Osório Leiria
12. Pedro M. Moraes-Vieira
13. Roger Frigério Castilho
14. Shingo Kajimura
15. Marcelo A. Mori
16. Licio A. Velloso
17. Carlos H. Sponton

Reviewed by Review Commons

Modeling metabolic disease susceptibility and resilience in genetically diverse mice

1. Candice N Baker
2. Jeffrey M Harder
3. Daniel A Skelly
4. Isabella Gerdes Gyuricza
5. Margaret Gaca
6. Matthew Vincent
7. Allison Ingalls
8. Mark P Keller
9. Alan D Attie
10. Madeleine Braun
11. Michael Stitzel
12. Edison T Liu
13. Nadia Rosenthal
14. Gary A Churchill

• Curated by eLife

  eLife Assessment

  The authors used three genetically diverse mouse models to investigate the impact of genome diversity on metabolic disease outcomes, such as obesity and glucose tolerance. This study is important because it integrates comprehensive metabolic analyses and multi-tissue phenotyping across sexes to reveal pathways relevant to obesity and its complications; the data are convincing and uncover several pathways that advance understanding of disease etiology while suggesting potential therapeutic avenues to prevent obesity-related health risks. There are limitations, such as a limited number of mouse strains used in the work, the 9-week feeding regime may be too short to capture full metabolic remodeling, and the mechanisms by which the immune-adipose axis impacts the broader phenotype are not fully described. Overall, the study is compelling, but the manuscript could be improved by justifying the strain selection, addressing the concern about the feeding duration, and providing stronger mechanistic support or discussion.

Reviewed by eLife