Physiology

Loss of CTRP10 results in female obesity with preserved metabolic health

1. Fangluo Chen
2. Dylan C Sarver
3. Muzna Saqib
4. Leandro M Velez
5. Susan Aja
6. Marcus M Seldin
7. G William Wong

• Curated by eLife

  eLife Assessment

  This manuscript presents a detailed characterization of male and female wildtype and Ctrp10 knockout mice, and reveals that knockout mice develop female-specific obesity that is largely uncoupled from metabolic dysfunction. The data are convincing, and the work will be an important contribution to understanding how obesity is coupled to metabolic dysfunction, and how this can occur in a sex-specific manner.

Reviewed by eLife

Adiponectin exerts sex-dependent effects on lipid, amino acid, and glucose metabolism during caloric restriction

1. Yoshiko M Ikushima
2. Kuan-Chan Chen
3. Richard J. Sulston
4. Domenico Mattiucci
5. Eleanor J. Brain
6. Stefanie A Fung Xin Zi
7. Karla J. Suchacki
8. Benjamin J. Thomas
9. Andrea Lovdel
10. Matthew Bennett
11. Hiroshi Kobayashi
12. Phillip D. Whitfield
13. Keiyo Takubo
14. Andrew H. Baker
15. Nicholas M. Morton
16. Robert K. Semple
17. William P. Cawthorn

Reviewed by PREreview

The Kv2.2 channel mediates the inhibition of prostaglandin E2 on glucose-stimulated insulin secretion in pancreatic β-cells

1. Chengfang Pan
2. Ying Liu
3. Liangya Wang
4. Wen-Yong Fan
5. Yunzhi Ni
6. Xuefeng Zhang
7. Di Wu
8. Chenyang Li
9. Jin Li
10. Zhaoyang Li
11. Rui Liu
12. Changlong Hu

• Curated by eLife

  eLife Assessment

  The study presents valuable findings on the molecular mechanisms of glucose-stimulated insulin secretion from pancreatic islets, focusing on the main regulatory elements of the signaling pathway in physiological conditions. While the evidence supporting the conclusions is solid, the study can be strengthened by the use of a beta cell line or knockout mice. The work will be of interest to cell biologists and biochemists working on diabetes.

Reviewed by eLife

Multi-dimensional oscillatory activity of mouse GnRH neurons in vivo

1. Su Young Han
2. Shel-Hwa Yeo
3. Jae-Chang Kim
4. Ziyue Zhou
5. Allan E Herbison

• Curated by eLife

  eLife Assessment

  This valuable study investigates the oscillatory activity of gonadotropin-releasing hormone (GnRH) neurones in mice using GCaMP fiber photometry. It demonstrates three distinct patterns of oscillatory activity that occur in GnRH neurons comprising low-level rapid baseline activity, abrupt short-duration oscillations that drive pulsatile gonadotropin secretion, and, in females, a gradual and prolonged oscillating increase in activity responsible for the relatively short-lived preovulatory LH surge. The evidence presented in the study is solid, offering theoretical implications for understanding the behaviour of GnRH neurones in the context of reproductive physiology, and will be of interest to researchers in neuroendocrinology and reproductive biology.

Reviewed by eLife

Exercise training at different intensities induces heat stress, disrupts barrier function and alters microbiota in the gut of mice

1. Puqiao Lian
2. Artemiy Kovynev
3. Lei Wang
4. Amanda CM Pronk
5. Aswin Verhoeven
6. Martin Giera
7. Suzan Thijssen
8. Borja Martínez Téllez
9. Sander Kooijman
10. Patrick CN Rensen
11. Harro Timmerman
12. Harry J Wichers
13. Paul AJ Henricks
14. Gert Folkerts
15. Milena Schönke
16. Saskia Braber

• Curated by eLife

  eLife Assessment

  This valuable study examines how different exercise training intensities affect intestinal barrier function and gut microbiota composition over a 6-week period in mice. The evidence supporting the main claims about exercise-induced intestinal injury and microbiota changes is solid, featuring comprehensive histological analysis, molecular characterization, and metabolomic profiling, though key mechanistic insights and causal relationships remain to be established. The findings have practical implications for understanding exercise-induced gastrointestinal stress, particularly the observation that daily moderate exercise may be more damaging to intestinal integrity than vigorous exercise with rest days. Additional experimental validation would strengthen these conclusions.

Reviewed by eLife

Impaired myofibroblast proliferation is a central feature of pathologic post-natal alveolar simplification

1. Imran S Khan
2. Christopher Molina
3. Xin Ren
4. Vincent C Auyeung
5. Max Cohen
6. Tatsuya Tsukui
7. Amha Atakilit
8. Dean Sheppard

• Curated by eLife

  eLife Assessment

  This study provides important insights into postnatal lung development and the mechanisms underlying bronchopulmonary dysplasia (BPD), a condition with high morbidity and mortality in newborns. Through the use of neonatal hyperoxia, cell-type-specific inactivation of Tgfbr2, and other injury models, the research focuses on the role of TGF-β signaling in BPD pathogenesis, highlighting impaired myofibroblast proliferation as a key factor. The inactivation of Etc2 in Pdgfra-lineaged cells disrupts myofibroblast cytokinesis, leading to alveolar simplification and reduced cell numbers. The use of transgenic mice and single-cell transcriptomics offers a detailed and high-quality dataset, advancing our understanding of BPD and serving as a invaluable resource for developmental biology and neonatal pulmonary research. The study's comprehensive approach, robust data, and methodological rigor make it a compelling contribution to the field, providing both mechanistic insights and a resource for further research into BPD pathogenesis.

Reviewed by eLife

Cytosolic S100A8/A9 promotes Ca2+ supply at LFA-1 adhesion clusters during neutrophil recruitment

1. Matteo Napoli
2. Roland Immler
3. Ina Rohwedder
4. Valerio Lupperger
5. Johannes Pfabe
6. Mariano Gonzalez Pisfil
7. Anna Yevtushenko
8. Thomas Vogl
9. Johannes Roth
10. Melanie Salvermoser
11. Steffen Dietzel
12. Marjan Slak Rupnik
13. Carsten Marr
14. Barbara Walzog
15. Markus Sperandio
16. Monika Pruenster

• Curated by eLife

  eLife Assessment

  This important study investigates the contribution of cytosolic S100A/8 to neutrophil migration to inflamed tissues. The authors provide convincing evidence for how the loss of cytosolic S100A/8 specifically affects the ability of neutrophils to crawl and subsequently adhere under shear stress. This study will be of interest in fields where inflammation is implicated, such as autoimmunity or sepsis.

Reviewed by eLife

SLC35G1 is a highly chloride-sensitive transporter responsible for the basolateral membrane transport in intestinal citrate absorption

1. Yoshihisa Mimura
2. Tomoya Yasujima
3. Katsuhisa Inoue
4. Shogo Akino
5. Chitaka Namba
6. Hiroyuki Kusuhara
7. Yutaro Sekiguchi
8. Kinya Ohta
9. Takahiro Yamashiro
10. Hiroaki Yuasa

• Curated by eLife

  eLife Assessment

  This work identifies the molecular function of an orphan human transporter, SLC35G1, providing convincing evidence that this protein is involved in intestinal citrate absorption. This work provides important insight into transporter function and human physiology.

Reviewed by eLife

Dapagliflozin improves endothelial integrity and hemodynamics in endotoxin treated mice through an apolipoprotein M dependent pathway

1. Carla Valenzuela Ripoll
2. Zhen Guo
3. Tripti Kumari
4. Kana N. Miyata
5. Mualla Ozcan
6. Ahmed Diab
7. Amanda Girardi
8. Li He
9. Attila Kovacs
10. Carla Weinheimer
11. Jess Nigro
12. Jan Oscarsson
13. Russell Esterline
14. Joel Schilling
15. Mikhail Kosiborod
16. Christina Christoffersen
17. Jaehyung Cho
18. Ali Javaheri

• Curated by eLife

  eLife Assessment

  SGLT2 inhibitors (SGLT2i) have assumed important roles in reducing cardiovascular risk, particularly in those with diabetes. It has become appreciated that its protective effects are likely beyond their ability to lower blood sugar levels. This research presents a novel approach to studying the SGLT2i mechanism of action which is yet to be fully elucidated.

Reviewed by eLife

Astrocyte aquaporin mediates a tonic water efflux maintaining brain homeostasis

1. Cuong Pham
2. Yuji Komaki
3. Anna Deàs-Just
4. Benjamin Le Gac
5. Christine Mouffle
6. Clara Franco
7. Agnès Chaperon
8. Vincent Vialou
9. Tomokazu Tsurugizawa
10. Bruno Cauli
11. Dongdong Li

• Curated by eLife

  eLife Assessment

  In this work, the authors propose that astrocytic aquaporin 4 (AQP4) is the main pathway for tonic water efflux, without which astrocytes undergo cell swelling. These findings are important, because they shed light on key molecular mechanisms implicated with the regulation of brain water homeostasis. The authors use a broad set of experimental tools (e.g., acute brain slices, in vivo recording, and diffusion-weighted MRI) but the evidence remains incomplete without ruling out non-specific effects of TGN-020, and without evidence that changes in sulforhodamine B fluorescence can be used as reliable readouts of cell volume dynamics.

Reviewed by eLife