Physiology

PIEZO channels link mechanical forces to uterine contractions in parturition

1. Yunxiao Zhang
2. Sejal A. Kini
3. Sassan A. Mishkanian
4. Renhao Luo
5. Saba Heydari Seradj
6. Verina H. Leung
7. Yu Wang
8. M. Rocío Servín-Vences
9. William T. Keenan
10. Utku Sonmez
11. Oleg Yarishkin
12. Manuel Sanchez-Alavez
13. Yuejia Liu
14. Xin Jin
15. Darren J. Lipomi
16. Li Ye
17. Michael Petrascheck
18. Antonina I. Frolova
19. Sarah K. England
20. Ardem Patapoutian

Reviewed by PREreview

Roles of G-protein coupled receptors and mechanosensitive ion channels in pressure-induced chronotropy of lymphatic vessels

1. Michael J Davis
2. Hae Jin Kim
3. Min Li
4. Jorge A Castorena-Gonzalez
5. Soumiya Pal
6. Timothy L Domeier
7. Joshua P Scallan
8. Scott Earley
9. Scott D Zawieja

• Curated by eLife

  eLife Assessment

  Davis and colleagues describe findings that are fundamental to the understanding of pressure mechanosensation in lymphatic vessels and are of significant importance to other areas of mechanosensory physiology. Based on many different knockout mouse models and rigorous state-of-the-art pressure myography recordings, they present compelling evidence that mechano-activation of GNAQ/GNA11-coupled GPCRs generates IP3, which induces Ca2+ release from internal stores through IP3R1 and drives depolarization through the activation of ANO1 Cl- channels to induce lymphatic vessel contractility. Nevertheless, some aspects of the manuscript are incomplete. The specific identity of the GPCR(s) involved remains to be uncovered, as evidence of frequency-pressure impairment is only demonstrated with abolition of GNAQ/GNA11action, not the receptors per se.

Reviewed by eLife

Progressive postnatal hearing development limits early parent-offspring vocal communication in the zebra finch

1. Tommi Anttonen
2. Jakob Christensen-Dalsgaard
3. Coen PH Elemans

• Curated by eLife

  eLife Assessment

  Zebra finches are a prominent model system for vocal learning and auditory system function, yet little is known about the functional development of the auditory system. Here, the authors convincingly show that newly hatched zebra finches lack detectable auditory brainstem responses and that auditory neural signals emerge only days after hatching, challenging influential claims of prenatal acoustic communication in altricial birds. This important work clarifies the developmental timeline for auditory communication and highlights the value of neuroscientific methods for validating and complementing behavioral ecological studies of animal perception.

Reviewed by eLife

NAD+ boosting by oral nicotinamide mononucleotide administration regulates key metabolic and immune pathways through SIRT1 dependent and independent mechanisms to mitigate diet-induced obesity and dyslipidemia in mice

1. Yasser Majeed
2. Najeeb M Halabi
3. Rudolf Engelke
4. Hina Sarwath
5. Muna N Al-Noubi
6. Sunkyu Choi
7. Aisha Al-Malki
8. Maha V Agha
9. Muneera Vakayil
10. Lotfi Chouchane
11. Frank Schmidt
12. Nayef A Mazloum

• Curated by eLife

  eLife Assessment

  The mechanistic basis for the potential health benefits of NAD⁺ precursors remains incompletely understood. This manuscript provides a useful assessment of the role of SIRT1 in mediating the effects of NMN in mice fed a high-fat diet. The study addresses a key question, though some of the conclusions appear only partially supported by the presented data.

Reviewed by eLife

Anti-diuretic hormone ITP signals via a guanylate cyclase receptor to modulate systemic homeostasis in Drosophila

1. Jayati Gera
2. Marishia Agard
3. Hannah Nave
4. Austin B Baldridge
5. Farwa Sajadi
6. Leena Thorat
7. Theresa H McKim
8. Shu Kondo
9. Dick R Nässel
10. Mitchell H Omar
11. Jean-Paul Paluzzi
12. Meet Zandawala

• Curated by eLife

  eLife Assessment

  The authors used comprehensive approaches to identify Gyc76C as an ITPa receptor in Drosophila. They revealed that ITPa acts via Gyc76C in the renal tubules and fat body to modulate osmotic and metabolic homeostasis. The designed experiments, data, and analyses convincingly support the main claims. The findings are important to help us better understand how ITP signals contributes to systemic homeostasis regulation.

Reviewed by eLife

E4BP4 Safeguards Brown Fat Mitochondria from Obesity-Induced Fragmentation via Ceramide Repression

1. Fernando Valdivieso-Rivera
2. Vanessa O. Furino
3. Carlos E. Leher
4. Ariane M. Zanesco
5. Monara Kaélle Cruz
6. Flavia C. Gan
7. Adriana Leandra Santoro
8. Lara Regina-Ferreira
9. Giovanna Leite Santos
10. Tiago Gonçalves
11. Luiz Osório Leiria
12. Pedro M. Moraes-Vieira
13. Roger Frigério Castilho
14. Shingo Kajimura
15. Marcelo A. Mori
16. Licio A. Velloso
17. Carlos H. Sponton

Reviewed by Review Commons

Modeling metabolic disease susceptibility and resilience in genetically diverse mice

1. Candice N Baker
2. Jeffrey M Harder
3. Daniel A Skelly
4. Isabella Gerdes Gyuricza
5. Margaret Gaca
6. Matthew Vincent
7. Allison Ingalls
8. Mark P Keller
9. Alan D Attie
10. Madeleine Braun
11. Michael Stitzel
12. Edison T Liu
13. Nadia Rosenthal
14. Gary A Churchill

• Curated by eLife

  eLife Assessment

  The authors used three genetically diverse mouse models to investigate the impact of genome diversity on metabolic disease outcomes, such as obesity and glucose tolerance. This study is important because it integrates comprehensive metabolic analyses and multi-tissue phenotyping across sexes to reveal pathways relevant to obesity and its complications; the data are convincing and uncover several pathways that advance understanding of disease etiology while suggesting potential therapeutic avenues to prevent obesity-related health risks. There are limitations, such as a limited number of mouse strains used in the work, the 9-week feeding regime may be too short to capture full metabolic remodeling, and the mechanisms by which the immune-adipose axis impacts the broader phenotype are not fully described. Overall, the study is compelling, but the manuscript could be improved by justifying the strain selection, addressing the concern about the feeding duration, and providing stronger mechanistic support or discussion.

Reviewed by eLife

Megakaryocytes assemble a three-dimensional cage of extracellular matrix that controls their maturation and anchoring to the vascular niche

1. Claire Masson
2. Cyril Scandola
3. Jean-Yves Rinckel
4. Fabienne Proamer
5. Emily Janus-Bell
6. Fareeha Batool
7. Naël Osmani
8. Jacky G Goetz
9. Léa Mallo
10. Nathalie Brouard
11. Catherine Leon
12. Alicia Bornert
13. Renaud Poincloux
14. Olivier Destaing
15. Alma Mansson
16. Hong Qian
17. Maxime Lehmann
18. Anita Eckly

• Curated by eLife

  eLife Assessment:

  In this revised version, the authors provide a thorough investigation of the interaction of megakaryocytes (MK) with their associated extracellular matrix (ECM) during maturation; they provide compelling evidence that the existence of a dense cage-like pericellular structure containing laminin γ1 and α4 and collagen IV is key to fixing the perisinusoidal localization of MK and preventing their premature intravasation. Adhesion of MK to this ECM cage is dependent on integrin beta1 and beta3 expressed by MK. This strong conclusion is based on the use of state-of-the art techniques such f primary murine bone marrow MK cultures, mice lacking ECM receptors, namely integrin beta1 and beta3 null mice, as well as high-resolution 2D and 3D imaging. The study provides valuable insight into the role of cell-matrix interactions in MK maturation and provides an interesting model with practical implications for the fields of hemostasis and thrombosis.

Reviewed by eLife, preLights

The denitrosylase SCoR2 controls cardioprotective metabolic reprogramming

1. Zachary W Grimmett
2. Rongli Zhang
3. Hua-Lin Zhou
4. Qiuying Chen
5. Dawson Miller
6. Zhaoxia Qian
7. Justin Lin
8. Riti Kalra
9. Steven S Gross
10. Walter J Koch
11. Richard T Premont
12. Jonathan S Stamler

• Curated by eLife

  eLife Assessment

  This study provides new and interesting findings that SCoR2 acts as a denitrosylase to control cardioprotective metabolic reprogramming and prevent injury following ischemia/reperfusion. The compelling evidence is supported by a novel multi-omics approach, but questions remain regarding the stability and human relevance of BDH1 as well as the sufficiency of SCoR2. Overall, the work will be of interest to cardiovascular researchers and provides valuable information to the field, though some mechanistic aspects require further clarification.

Reviewed by eLife

Neuronal detection triggers systemic digestive shutdown in response to adverse food sources in Caenorhabditis elegans

1. Yating Liu
2. Guojing Tian
3. Ziyi Wang
4. Junkang Zheng
5. Huimin Liu
6. Sucheng Zhu
7. Zhao Shan
8. Bin Qi

• Curated by eLife

  eLife Assessment

  This important study investigates how signals from the nervous system can influence the response to different food sources. To demonstrate the role of specific neuronal and intestinal regulators in sensing food quality and modulating digestion, the authors present evidence through a combination of genetic screening, RNA-seq analysis, and functional studies. These findings shed light on an adaptive strategy to integrate food perception with physiological responses, with a mix of solid and convincing evidence supporting the work.

Reviewed by eLife