Physiology

Transient hypoxia followed by progressive reoxygenation is required for efficient skeletal muscle repair through Rev-ERBα modulation

1. Marie Quétin
2. Audrey Der Vartanian
3. Christelle Dubois
4. Juliette Berthier
5. Marine Ledoux
6. Stéphanie Michineau
7. Bernadette Drayton-Libotte
8. Athanassia Sotiropoulos
9. Frédéric Relaix
10. Marianne Gervais

Reviewed by Review Commons

Probing relaxed myosin states in hypertrophic cardiomyopathy by second harmonic-generation microscopy

1. Giulia Arecchi
2. Marica Dente
3. Weikang Ma
4. Beatrice Scellini
5. Nicoletta Piroddi
6. Marina Scardigli
7. Jingyuan Yu
8. Jing Zhao
9. Riccardo Cicchi
10. Ryo Kinegawa
11. Caroline Muellenbroich
12. Corrado Poggesi
13. Cecilia Ferrantini
14. Thomas C Irving
15. Michael Regnier
16. Leonardo Sacconi
17. Chiara Tesi

• Curated by eLife

  eLife Assessment

  Arecchi et al. demonstrate that polarized second-harmonic generation microscopy can be used to probe the ON/OFF states of myosin in both permeabilized and intact muscle, making this key measurement accessible to a greater number of labs. This has the potential to help with the study of disease-causing mutations and our understanding of drug function. The methodology is well defined, and the results are important; however, whilst this is overall a convincing study, there are some limitations to the interpretation of the data.

Reviewed by eLife

Cellular characterization of the mouse collecting lymphatic vessels reveals that lymphatic muscle cells are the innate pacemaker cells

1. Scott D Zawieja
2. Grace A Pea
3. Sarah E Broyhill
4. Advaya Patro
5. Karen H Bromert
6. Charles E Norton
7. Hae Jin Kim
8. Sathesh Kumar Sivasankaran
9. Min Li
10. Jorge A Castorena-Gonzalez
11. Bernard T Drumm
12. Michael J Davis

• Curated by eLife

  eLife Assessment

  This manuscript aims to identify the pacemaker cells in the lymphatic collecting vessels - the cells that initiate the autonomous action potentials and contractions needed to drive lymphatic pumping. Through the exemplary use of existing approaches (genetic deletions and cytosolic calcium detection in multiple cell types), the authors convincingly determine that lymphatic muscle cells are the origin of the action potential that triggers lymphatic contraction. The inclusion of scRNAseq and membrane potential data enhances a tremendous study. This fundamental discovery establishes a new standard for the field of lymphatic physiology.

Reviewed by eLife

Differential Regulation of Hepatic Macrophage Fate by Chi3l1 in MASLD

1. Jia He
2. Bo Chen
3. Xiong Wang
4. Ruoxue Yang
5. Chengxiang Deng
6. Xiane Zhu
7. Keqin Wang
8. Lang Wang
9. Xiaokang Lu
10. Cheng Peng
11. Canpeng Li
12. Zhao Shan

• Curated by eLife

  eLife Assessment

  This is a useful study in the role of CHI3L1 in Kupffer cells, the macrophages of the liver, showing that CHI3L1 alters glucose regulation in obesity. Specifically, Chi3l1 protects glucose-dependent Kupffer cells during Metabolic dysfunction-associated steatotic liver disease (MASLD) by inhibiting glucose uptake, preventing metabolic stress and death. These data are compelling, yet require further validation.

Reviewed by eLife

Cell-autonomous and non-cell-autonomous effects of Arginase 2 on cardiac aging

1. Duilio M Potenza
2. Xin Cheng
3. Guillaume Ajalbert
4. Andrea Brenna
5. Marie-Noelle Giraud
6. Aurelien Frobert
7. Stephane Cook
8. Kirsten D Mertz
9. Zhihong Yang
10. Xiu-Fen Ming

• Curated by eLife

  eLife Assessment

  This study provides fundamental information on how Arg-II participates in cardiac aging. The phenotypic data provide convincing evidence of non-cell-autonomous contributions to aging-related pathologies. Overall, the study highlights the importance of intercellular signaling in maintaining cardiac health during aging.

Reviewed by eLife

TRPV4 activation by TGFβ2 enhances cellular contractility and drives ocular hypertension

1. Christopher Nass Rudzitis
2. Monika Lakk
3. Ayushi Singh
4. Sarah N Redmon
5. Denisa Kirdajová
6. Yun-Ting Tseng
7. Michael L De Ieso
8. W Daniel Stamer
9. Samuel Herberg
10. David Krizaj

• Curated by eLife

  eLife Assessment

  This fundamental work extends our understanding of the role of TGFβ2 as a modulator of mechanosensing in the eye and identifies the TRPV4 ion channel as a common regulator of Trabecular Meshwork (TM) contractility and pathological OHT and the data and evidence provided are convincing. This work will clearly be of interest to researchers investigating the role of mechanosensors in the TM and may underpin future research into treatments that aim to lower intra ocular pressure. This work will additionally be of interest to the growing field of researchers investigating the regulation of force sensing via ion channels and their roles in health and disease, in particular the ion channel TRPV4.

Reviewed by eLife

A whole-animal phenotypic drug screen identifies suppressors of atherogenic lipoproteins

1. Daniel J Kelpsch
2. Liyun Zhang
3. James H Thierer
4. Kobe Koren
5. Urmi Kumar
6. Yuki Lin
7. Monica R Hensley
8. Mira Sohn
9. Jun O Liu
10. Thomas Lectka
11. Jeff S Mumm
12. Steven A Farber

• Curated by eLife

  eLife Assessment

  In this important study, the authors have performed a zebrafish drug screen to identify suppressors of atherogenic lipoproteins. They utilize a well-established LipoGlo assay to find molecules that modulate these lipoproteins, identifying 49 potential hits. They perform some validation experiments, including studies linking enoxolone to its likely inhibitory effect on a specific transcription factor, HNF4alpha. Overall, the results are convincing and robust, and will open up new areas of exploration for those investigators interested in in vivo lipid biology.

Reviewed by eLife

Effects of Arginine Vasopressin on Islet Cells in Pancreatic Tissue Slices: Glucose-Dependent Modulation of IP3 Receptor-Specific Responses

1. Jasmina Kerčmar
2. Nastja Murko
3. Lidija Križančić Bombek
4. Eva Paradiž Leitgeb
5. Johannes Pfabe
6. Sandra Postić
7. Ya-Chi Huang
8. Andraž Stožer
9. Dean Korošak
10. Xaver Kozisek
11. Monika Perisic
12. Markus Muttenthaler
13. Christian W Gruber
14. Marjan Slak Rupnik

• Curated by eLife

  eLife Assessment

  This study presents a useful finding on the effects of arginine vasopressin (AVP) on islet cells in pancreatic tissue slices, using technically sophisticated spatio-temporal calcium recordings to confirm that AVP influences α and β cells differently depending on glucose concentrations. While the study’s methods – particularly the calcium imaging techniques and peptide ligand design targeting V1b receptors – are strong, the reviewers were concerned about several aspects of the experimental design. The results on β-cell responses are incomplete and insufficient to support the manuscript’s claims, especially due to the high variability of islet responses and lack of mechanistic and functional (hormone release) data. There are also concerns about the possibility of off-target effects and suboptimal receptor specificity: the study would be significantly strengthened by inclusion of signaling pathway interrogation, hormone output assays, genetic validation (e.g., β cell-specific deletion of V1br), and receptor localization. The work will still be of interest to researchers studying islet physiology in the context of health and diabetes.

Reviewed by eLife

Sex-dependent effects of a gestational ketogenic diet on offspring birth and lifespan

1. Sarah M. Zala
2. Renata Santos
3. Eva Strasser
4. Alice Schadde
5. Sarah Kugler
6. Verena Strauss
7. Anna Kübber-Heiss
8. Diana Zala

Reviewed by PREreview

Iron-deplete diet enhances Caenorhabditis elegans lifespan via oxidative stress response pathways

1. Priyanka Das
2. Ravi
3. Jogender Singh

Reviewed by Review Commons