Physiology

Differential regulation of hepatic macrophage fate by Chi3l1 in metabolic dysfunction-associated steatotic liver disease

1. Jia He
2. Bo Chen
3. Weiju Lu
4. Xiong Wang
5. Ruoxue Yang
6. Chengxiang Deng
7. Xiane Zhu
8. Keqin Wang
9. Lang Wang
10. Cheng Xie
11. Rui Li
12. Xiaokang Lu
13. Ruizhi Yang
14. Cheng Peng
15. Canpeng Li
16. Zhao Shan

• Curated by eLife

  eLife Assessment

  This study provides an insight into the role of a Chi3l1 in liver macrophages during metabolic disease. The evidence is solid with the authors now addressing most concerns, although one key conclusion is not fully supported by the data presented. Overall, the work offers a useful contribution to the field.

Reviewed by eLife

Cytokine control of systemic hypoxia tolerance in Drosophila

1. Kate Ding
2. Prajakta Bodkhe
3. Byoungchun Lee
4. Danielle Polan
5. Amy Wycislik
6. Tiffany Cheung
7. Sophie Wu
8. Savraj S Grewal

Reviewed by Review Commons, PREreview

Zinc is a Key Regulator of the Sperm-Specific K+ Channel (Slo3) Function

1. Rizki Tsari Andriani
2. Tanadet Pipatpolkai
3. Haruhiko Miyata
4. Masahito Ikawa
5. Yasushi Okamura
6. Takafumi Kawai

• Curated by eLife

  eLife Assessment

  This study presents a valuable finding on the role of intracellular zinc as a regulator of the sperm-specific potassium channel Slo3, demonstrating that zinc export during capacitation contributes to alkalinization-induced membrane hyperpolarization. The electrophysiological evidence supporting zinc-mediated inhibition of Slo3 is solid, though the mechanistic basis of this inhibition is not complete, as the proposed zinc-binding site involving E169 and E205 has not been directly tested through double-mutant analysis. This work will be of interest to reproductive biologists and ion channel biophysicists studying the molecular mechanisms of sperm capacitation.

Reviewed by eLife

Natural xanthones as α-Mangostin induce vasorelaxation involving key gating residues in the S6 domain of BK channels

1. Soenke Cordeiro
2. Robert Patejdl
3. Thomas Baukrowitz
4. Marianne A Musinszki

• Curated by eLife

  eLife Assessment

  The present manuscript by Cordeiro et al., shows convincing evidence that α-mangostin, a xanthone obtained from the fruit of the Garcinia mangostana tree, behaves as a strong activator of the large-conductance (BK) potassium channels. The authors suggest that α-mangostin activation of the BK channel is state-independent, and molecular docking and mutagenesis suggest that α-mangostin binds to a site in the internal cavity. Additionally, the authors show that α-mangostin can relax arteries, further suggesting the plausibility of the proposed effects of this compound. These are valuable findings that should be of interest to channel biophysicists and physiologists alike.

Reviewed by eLife

Metabolism fine tuning and cardiokines secretion represent adaptative responses of the heart to High Fat and High Sugar Diets in flies

1. Lucie Khamvongsa-Charbonnier
2. Laurent Kremmer
3. Magali Torres
4. Sallouha Krifa
5. Charis Aubert
6. Alice Corbet
7. Loïc Crespo
8. Laurence Röder
9. Laurent Perrin
10. Nathalie Arquier

Reviewed by Review Commons

The Hypothalamic–Pituitary–Adrenal Axis Orchestrates Energy Homeostasis during Cold Exposure

1. Mireia Llerins Perez
2. Xinran Gao
3. Laura Heimerl
4. Yuanyuan Wang
5. Katharina Schnabl
6. Uwe Firzlaff
7. Harald Luksch
8. Martin Klingenspor
9. Nina Henriette Uhlenhaut
10. Dehua Wang
11. Yongguo Li

Reviewed by PREreview

Single transcript level atlas of oxytocin and the oxytocin receptor in the mouse brain

1. Vitaly Ryu
2. Anisa Azatovna Gumerova
3. Georgii Pevnev
4. Funda Korkmaz
5. Hasni Kannangara
6. Liam Cullen
7. Farhath Sultana
8. Ronit Witztum
9. Steven Lee Sims
10. Tal Frolinger
11. Ofer Moldavski
12. Orly Barak
13. Emily Weiss
14. Jay J Cao
15. Daria Lizneva
16. Ki A Goosens
17. Tony Yuen
18. Mone Zaidi

• Curated by eLife

  eLife Assessment

  This fundamental work substantially advances our understanding of OXT (oxytocin) neurons and OXTR (oxytocin receptor) expressions in mammalian brains using an advanced RNAscope at the single transcript level. The evidence supporting the conclusions is compelling using chromogenic assays and state-of-the-art microscopy. The work will be of broad interest to neuroscientists and endocrinologists.

Reviewed by eLife

Efficient transduction of pancreas tissue slices with genetically encoded calcium integrators

1. Charles S Lazimi
2. Austin E Stis
3. Julia K Panzer
4. Helmut Hiller
5. Maria L Beery
6. Amelia K Linnemann
7. Cherie L Stabler
8. Clayton E Mathews
9. Edward A Phelps

• Curated by eLife

  eLife Assessment

  This study presents a valuable advance by enabling functional mapping of Ca²⁺ responses in live human pancreatic tissue slices, providing new opportunities to study islet heterogeneity and diabetes-related dysfunction in an intact tissue context. The evidence supporting the main conclusions is solid, based on reproducible methodology and functional validation across multiple human donor samples. Key revisions needed include clearer quantification of transduction efficiency and tissue viability, and improved clarification of how CaMPARI2 signals should be interpreted.

Reviewed by eLife

Non-visual light modulates behavioral memory and gene expression in Caenorhabditis elegans

1. Zhijian Ji
2. Bingying Wang
3. Rashmi Chandra
4. Junqiang Liu
5. Supeng Yang
6. Yong Long
7. Michael Egan
8. Fujia Han
9. Han Wang
10. Noelle L'Etoile
11. Dengke K Ma

• Curated by eLife

  eLife Assessment

  This important study uncovers a previously unrecognized light-responsive pathway in C. elegans that depends on live food bacteria and is mediated by the bZIP factors ZIP-2/CEBP-2 and the cytochrome P450 enzyme, CYP-14A5. The authors show that this bacteria-linked pathway modulates long-term memory and can be harnessed as a low-cost light-inducible expression system, opening new directions for sensory biology and genetic engineering in worms. The exact means by which live bacteria modulate light signal that activates ZIP-2/CEBP-2 in the worm remains to be elucidated. The evidence supporting the pathway's role uses multiple genetic, transcriptional, and behavioural assays, and is convincing.

Reviewed by eLife

SLC4A1 mutations that cause distal renal tubular acidosis alter cytoplasmic pH and cellular autophagy

1. Grace Essuman
2. Midhat Rizvi
3. Ensaf Almomani
4. Shahid AKM Ullah
5. Sarder MA Hasib
6. Forough Chelangarimiyandoab
7. Priyanka Mungara
8. Manfred J Schmitt
9. Marguerite Hureaux
10. Rosa Vargas-Poussou
11. Nicolas Touret
12. Emmanuelle Cordat

• Curated by eLife

  eLife Assessment

  This work reports the characterization of newly identified genetic variants of SLC4A1 in patients with distal renal tubular acidosis. Cell culture studies supplemented with histological analysis of a previously established disease mouse model provide convincing evidence that some of the variants increase intracellular pH, reduce ATP synthesis, and attenuate autophagic degradative flux. The study is valuable in establishing a mechanistic framework for future exploration of the link between intracellular pH and mutations in SLC4A1 in vivo.

Reviewed by eLife