Physiology

The Hypothalamic–Pituitary–Adrenal Axis Orchestrates Energy Homeostasis during Cold Exposure

1. Mireia Llerins Perez
2. Xinran Gao
3. Laura Heimerl
4. Yuanyuan Wang
5. Katharina Schnabl
6. Uwe Firzlaff
7. Harald Luksch
8. Martin Klingenspor
9. Nina Henriette Uhlenhaut
10. Dehua Wang
11. Yongguo Li

Reviewed by PREreview

Single transcript level atlas of oxytocin and the oxytocin receptor in the mouse brain

1. Vitaly Ryu
2. Anisa Azatovna Gumerova
3. Georgii Pevnev
4. Funda Korkmaz
5. Hasni Kannangara
6. Liam Cullen
7. Farhath Sultana
8. Ronit Witztum
9. Steven Lee Sims
10. Tal Frolinger
11. Ofer Moldavski
12. Orly Barak
13. Emily Weiss
14. Jay J Cao
15. Daria Lizneva
16. Ki A Goosens
17. Tony Yuen
18. Mone Zaidi

• Curated by eLife

  eLife Assessment

  This fundamental work substantially advances our understanding of OXT (oxytocin) neurons and OXTR (oxytocin receptor) expressions in mammalian brains using an advanced RNAscope at the single transcript level. The evidence supporting the conclusions is compelling using chromogenic assays and state-of-the-art microscopy. The work will be of broad interest to neuroscientists and endocrinologists.

Reviewed by eLife

RiboTRAP-seq identifies spatially distinct functions for the anterior and posterior intestine in immune and metabolic regulation in C. elegans

1. Chung-Chih Liu
2. Nicolas Seban
3. Supriya Srinivasan

• Curated by eLife

  eLife Assessment

  In this valuable study, the authors performed cell-specific ribosome pulldown to identify gene expression (translatome) differences in the anterior (NT1) vs middle & posterior (NT2-9) cells of the C. elegans intestine, under fed, starved, or refeeding conditions. The data generated will be very helpful to the C. elegans community, and the evidence supporting the conclusions of the study is assessed to be solid. Some methodological caveats remain and are discussed.

Reviewed by eLife

Efficient transduction of pancreas tissue slices with genetically encoded calcium integrators

1. Charles S Lazimi
2. Austin E Stis
3. Julia K Panzer
4. Helmut Hiller
5. Maria L Beery
6. Amelia K Linnemann
7. Cherie L Stabler
8. Clayton E Mathews
9. Edward A Phelps

• Curated by eLife

  eLife Assessment

  This study presents a valuable advance by enabling functional mapping of Ca²⁺ responses in live human pancreatic tissue slices, providing new opportunities to study islet heterogeneity and diabetes-related dysfunction in an intact tissue context. The evidence supporting the main conclusions is solid, based on reproducible methodology and functional validation across multiple human donor samples. Key revisions needed include clearer quantification of transduction efficiency and tissue viability, and improved clarification of how CaMPARI2 signals should be interpreted.

Reviewed by eLife

Non-visual light modulates behavioral memory and gene expression in Caenorhabditis elegans

1. Zhijian Ji
2. Bingying Wang
3. Rashmi Chandra
4. Junqiang Liu
5. Supeng Yang
6. Yong Long
7. Michael Egan
8. Fujia Han
9. Han Wang
10. Noelle L'Etoile
11. Dengke K Ma

• Curated by eLife

  eLife Assessment

  This important study uncovers a previously unrecognized light-responsive pathway in C. elegans that depends on live food bacteria and is mediated by the bZIP factors ZIP-2/CEBP-2 and the cytochrome P450 enzyme, CYP-14A5. The authors show that this bacteria-linked pathway modulates long-term memory and can be harnessed as a low-cost light-inducible expression system, opening new directions for sensory biology and genetic engineering in worms. The exact means by which live bacteria modulate light signal that activates ZIP-2/CEBP-2 in the worm remains to be elucidated. The evidence supporting the pathway's role uses multiple genetic, transcriptional, and behavioural assays, and is convincing.

Reviewed by eLife

SLC4A1 mutations that cause distal renal tubular acidosis alter cytoplasmic pH and cellular autophagy

1. Grace Essuman
2. Midhat Rizvi
3. Ensaf Almomani
4. Shahid AKM Ullah
5. Sarder MA Hasib
6. Forough Chelangarimiyandoab
7. Priyanka Mungara
8. Manfred J Schmitt
9. Marguerite Hureaux
10. Rosa Vargas-Poussou
11. Nicolas Touret
12. Emmanuelle Cordat

• Curated by eLife

  eLife Assessment

  This work reports the characterization of newly identified genetic variants of SLC4A1 in patients with distal renal tubular acidosis. Cell culture studies supplemented with histological analysis of a previously established disease mouse model provide convincing evidence that some of the variants increase intracellular pH, reduce ATP synthesis, and attenuate autophagic degradative flux. The study is valuable in establishing a mechanistic framework for future exploration of the link between intracellular pH and mutations in SLC4A1 in vivo.

Reviewed by eLife

Sex-biased expression of enteroendocrine cell-derived hormones contributes to higher fat storage in Drosophila females

1. Puja Biswas
2. Elizabeth J Rideout

• Curated by eLife

  eLife Assessment

  This useful study provides a systematic and solid comparison of sex-biased enteroendocrine peptide expression, including AstC and Tk, to show that these peptides contribute to female-biased fat storage. The major research question of this study is based on the authors' previous papers, and therefore, the presented results are incremental. This study serves as a foundation for future investigation of regulatory mechanisms for the sex-biased fat content by AstC and Tk.

Reviewed by eLife

PIEZO channels link mechanical forces to uterine contractions in parturition

1. Yunxiao Zhang
2. Sejal A. Kini
3. Sassan A. Mishkanian
4. Renhao Luo
5. Saba Heydari Seradj
6. Verina H. Leung
7. Yu Wang
8. M. Rocío Servín-Vences
9. William T. Keenan
10. Utku Sonmez
11. Oleg Yarishkin
12. Manuel Sanchez-Alavez
13. Yuejia Liu
14. Xin Jin
15. Darren J. Lipomi
16. Li Ye
17. Michael Petrascheck
18. Antonina I. Frolova
19. Sarah K. England
20. Ardem Patapoutian

Reviewed by PREreview

Roles of G-protein coupled receptors and mechanosensitive ion channels in pressure-induced chronotropy of lymphatic vessels

1. Michael J Davis
2. Hae Jin Kim
3. Min Li
4. Jorge A Castorena-Gonzalez
5. Soumiya Pal
6. Timothy L Domeier
7. Joshua P Scallan
8. Scott Earley
9. Scott D Zawieja

• Curated by eLife

  eLife Assessment

  Davis and colleagues describe findings that are fundamental to the understanding of pressure mechanosensation in lymphatic vessels and are of significant importance to other areas of mechanosensory physiology. Based on many different knockout mouse models and rigorous state-of-the-art pressure myography recordings, they present compelling evidence that mechano-activation of GNAQ/GNA11-coupled GPCRs generates IP3, which induces Ca2+ release from internal stores through IP3R1 and drives depolarization through the activation of ANO1 Cl- channels to induce lymphatic vessel contractility. Nevertheless, some aspects of the manuscript are incomplete. The specific identity of the GPCR(s) involved remains to be uncovered, as evidence of frequency-pressure impairment is only demonstrated with abolition of GNAQ/GNA11action, not the receptors per se.

Reviewed by eLife

Progressive postnatal hearing development limits early parent-offspring vocal communication in the zebra finch

1. Tommi Anttonen
2. Jakob Christensen-Dalsgaard
3. Coen PH Elemans

• Curated by eLife

  eLife Assessment

  Zebra finches are a prominent model system for vocal learning and auditory system function, yet little is known about the functional development of the auditory system. Here, the authors convincingly show that newly hatched zebra finches lack detectable auditory brainstem responses and that auditory neural signals emerge only days after hatching, challenging influential claims of prenatal acoustic communication in altricial birds. This important work clarifies the developmental timeline for auditory communication and highlights the value of neuroscientific methods for validating and complementing behavioral ecological studies of animal perception.

Reviewed by eLife