Physiology

An acute microglial metabolic response controls metabolism and improves memory

1. Anne Drougard
2. Eric H Ma
3. Vanessa Wegert
4. Ryan Sheldon
5. Ilaria Panzeri
6. Naman Vatsa
7. Stefanos Apostle
8. Luca Fagnocchi
9. Judith Schaf
10. Klaus Gossens
11. Josephine Völker
12. Shengru Pang
13. Anna Bremser
14. Erez Dror
15. Francesca Giacona
16. Sagar
17. Michael X Henderson
18. Marco Prinz
19. Russell G Jones
20. J. Andrew Pospisilik

• Curated by eLife

  eLife assessment

  This important study demonstrates a link between an acute high fat diet, microglial metabolism and improved higher cognitive function. The evidence supporting the proposed mechanism in vivo is incomplete at this stage due to non-trivial technical limitations but the authors provide convincing in vitro metabolic characterization of primary microglia cultures to support the model. This work will be of interest to a broad audience in the field of neuroscience, metabolism, and immunology.

Reviewed by eLife

The Kv2.2 channel mediates the inhibition of Prostaglandin E2 on glucose-stimulated insulin secretion in pancreatic β-cells

1. Chengfang Pan
2. Ying Liu
3. Liangya Wang
4. Wengyong Fan
5. Yunzhi Ni
6. Xuefeng Zhang
7. Di Wu
8. Chenyang Li
9. Jin Li
10. Zhaoyang Li
11. Rui Liu
12. Changlong Hu

• Curated by eLife

  eLife assessment

  The study presents valuable findings on the molecular mechanisms of glucose-stimulated insulin secretion from pancreatic islets, focusing on the main regulatory elements of the signaling pathway in physiological conditions. While the evidence supporting the conclusions is solid, the study can be strengthened by the use of a beta cell line or knockout mice. The work will be of interest to cell biologists and biochemists working on diabetes.

Reviewed by eLife

Cytosolic S100A8/A9 promotes Ca ²⁺ supply at LFA-1 adhesion clusters during neutrophil recruitment

1. Matteo Napoli
2. Roland Immler
3. Ina Rohwedder
4. Valerio Lupperger
5. Johannes Pfabe
6. Mariano Gonzalez Pisfil
7. Anna Yevtushenko
8. Thomas Vogl
9. Johannes Roth
10. Melanie Salvermoser
11. Steffen Dietzel
12. Marjan Slak Rupnik
13. Carsten Marr
14. Barbara Walzog
15. Markus Sperandio
16. Monika Pruenster

• Curated by eLife

  eLife assessment:

  This important study investigates the contribution of cytosolic S100A/8 to neutrophil migration to inflamed tissues. The authors provide convincing evidence for how the loss of cytosolic S100A/8 specifically affects the ability of neutrophils to crawl and subsequently adhere under shear stress. This study will be of interest in fields where inflammation is implicated, such as autoimmunity or sepsis.

Reviewed by eLife

SLC35G1: A highly chloride-sensitive transporter responsible for the basolateral membrane transport in intestinal citrate absorption

1. Yoshihisa Mimura
2. Tomoya Yasujima
3. Katsuhisa Inoue
4. Shogo Akino
5. Chitaka Namba
6. Hiroyuki Kusuhara
7. Yutaro Sekiguchi
8. Kinya Ohta
9. Takahiro Yamashiro
10. Hiroaki Yuasa

• Curated by eLife

  eLife assessment

  This work identifies the molecular function of an orphan human transporter, SLC35G1, providing convincing but somewhat incomplete evidence that this protein is involved in intestinal citrate absorption. This work provides important insight into transporter function and human physiology.

Reviewed by eLife

Genetic code expansion, click chemistry, and light-activated PI3K reveal details of membrane protein trafficking downstream of receptor tyrosine kinases

1. Duk-Su Koh
2. Anastasiia Stratiievska
3. Subhashis Jana
4. Shauna C Otto
5. Teresa M Swanson
6. Anthony Nhim
7. Sara Carlson
8. Marium Raza
9. Ligia Araujo Naves
10. Eric N Senning
11. Ryan A Mehl
12. Sharona E Gordon

• Curated by eLife

  eLife assessment

  This study develops a new and important method for dissecting out two overlapping cell signaling pathways, phosphoinositide signaling and membrane protein trafficking. The combination of two state-of-the-art spectroscopic techniques provides compelling evidence for a reciprocal influence between an enzyme and a channel. The work will be of interest to the broader cell biology, biophysics and biochemistry communities.

Reviewed by eLife

Reliance on self-reports and estimated food composition data in nutrition research introduces significant bias that can only be addressed with biomarkers

1. Javier I Ottaviani
2. Virag Sagi-Kiss
3. Hagen Schroeter
4. Gunter GC Kuhnle

• Curated by eLife

  eLife assessment

  This important study, using three bioactive compounds as a model, demonstrates that estimating the intake of food components based on food composition databases and self-reported dietary data is highly unreliable. The authors present convincing data showing the differences in the estimated quantile of intake of three bioactive compounds between biomarker and 24-hour dietary recall with food-composition database. The work will be of broad interest to the clinical nutrition research community.

Reviewed by eLife

ATP and glutamate coordinate contractions in the freshwater sponge Ephydatia muelleri

1. Vanessa R Ho
2. Greg G Goss
3. Sally P Leys

Reviewed by Arcadia Science

Neuroestrogens facilitate male-typical behaviors by potentiating androgen receptor signaling in medaka

1. Yuji Nishiike
2. Shizuku Maki
3. Daichi Miyazoe
4. Kiyoshi Nakasone
5. Yasuhiro Kamei
6. Takeshi Todo
7. Tomoko Ishikawa-Fujiwara
8. Kaoru Ohno
9. Takeshi Usami
10. Yoshitaka Nagahama
11. Kataaki Okubo

• Curated by eLife

  eLife assessment

  The authors provide an important step forward in understanding how brain-derived hormones modulate behavior, using medaka fish as a model system. Knockout lines present convincing evidence from multiple mutant lines, showing that estrogens play a significant role in male social behavior, and that lacking aromatase changes brain gene expression. The conclusions for females are less substantiated, and the conclusions regarding sexual differentiation should be considered carefully.

Reviewed by eLife

Involvement of TRPV4 in temperature-dependent perspiration in mice

1. Makiko Kashio
2. Sandra Derouiche
3. Reiko U Yoshimoto
4. Kenji Sano
5. Jing Lei
6. Mizuho A Kido
7. Makoto Tominaga

• Curated by eLife

  eLife assessment

  This useful studying implicates TRPV4 as a mediator of sweat, potentially based on TRPV4's expression and function on sweat glands. The data and methods are solid, with some limitations in terms of the approach. Overall, the work lends new insight into the physiologic basis of sweating using data from mice and humans.

Reviewed by eLife

A 2-hydroxybutyrate-mediated feedback loop regulates muscular fatigue

1. Brennan J Wadsworth
2. Marina Leiwe
3. Eleanor A Minogue
4. Pedro P Cunha
5. Viktor Engman
6. Carolin Brombach
7. Christos Asvestis
8. Shiv K Sah-Teli
9. Emilia Marklund
10. Peppi Koivunen
11. Jorge L Ruas
12. Helene Rundqvist
13. Johanna T Lanner
14. Randall S Johnson

• Curated by eLife

  eLife assessment

  The work by Johnson and co-workers has identified an important role of 2-Hydroxybutyrate in skeletal muscle oxidative capacity in the early stages of exercise. Mechanistically, they show convincing data to support a role of 2-Hydroxybutyrate in the regulation of BCAA metabolism via SIRT4, ADP-Ribosylation, and CEBP. However, whether this is the sole mechanism and if these translate to longer exercise training regimes requires future experiments.

Reviewed by eLife