Physiology

P. falciparum K13 mutations present varying degrees of artemisinin resistance and reduced fitness in African parasites

1. Barbara H. Stokes
2. Kelly Rubiano
3. Satish K. Dhingra
4. Sachel Mok
5. Judith Straimer
6. Nina F. Gnädig
7. Jade R. Bath
8. Ioanna Deni
9. Kurt E. Ward
10. Josefine Striepen
11. Tomas Yeo
12. Leila S. Ross
13. Eric Legrand
14. Frédéric Ariey
15. Clark H. Cunningham
16. Issa M. Souleymane
17. Adama Gansané
18. Romaric Nzoumbou-Boko
19. Claudette Ndayikunda
20. Abdunoor M. Kabanywanyi
21. Aline Uwimana
22. Samuel J. Smith
23. Olimatou Kolley
24. Mathieu Ndounga
25. Marian Warsame
26. Rithea Leang
27. François Nosten
28. Timothy J.C. Anderson
29. Philip J. Rosenthal
30. Didier Ménard
31. David A. Fidock

• Curated by eLife

  Evaluation Summary:

  This study is of interest to the broad malaria research community and especially those who work on drug resistance. The authors provide a summary of their surveys of African and Southeast Asian Plasmodium falciparum parasites for the Kelch 13 gene, a marker of artemisinin resistance. The contribution of several K13 mutations to artemisinin resistance is investigated in different genetic backgrounds and confirms the lack of a barrier for the potential emergence of artemisinin resistance in African parasites. These findings are of prime importance in the context of public health perspective on managing the risk of resistance appearing in Africa.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Combustible and Electronic Cigarette Exposures Increase ACE2 Activity and SARS-CoV-2 Spike Binding

1. Arunava Ghosh
2. Vishruth Girish
3. Monet Lou Yuan
4. Raymond D. Coakley
5. Joe A. Wrennall
6. Neil E. Alexis
7. Erin L. Sausville
8. Anand Vasudevan
9. Alexander R. Chait
10. Jason M. Sheltzer
11. Robert Tarran

Reviewed by ScreenIT

Multiple introductions of multidrug-resistant typhoid associated with acute infection and asymptomatic carriage, Kenya

1. Samuel Kariuki
2. Zoe A Dyson
3. Cecilia Mbae
4. Ronald Ngetich
5. Susan M Kavai
6. Celestine Wairimu
7. Stephen Anyona
8. Naomi Gitau
9. Robert Sanaya Onsare
10. Beatrice Ongandi
11. Sebastian Duchene
12. Mohamed Ali
13. John David Clemens
14. Kathryn E Holt
15. Gordon Dougan

Reviewed by eLife

The anti-immune dengue subgenomic flaviviral RNA is found in vesicles in mosquito saliva and associated with increased infectivity

1. Shih-Chia Yeh
2. Wei-Lian Tan
3. Avisha Chowdhury
4. Vanessa Chuo
5. R. Manjunatha Kini
6. Julien Pompon
7. Mariano A. Garcia-Blanco

• Curated by eLife

  Evaluation Summary:

  Mosquito saliva can enhance transmission of arboviruses. Here, authors demonstrated that the anti-immune non-coding RNA from Dengue virus, known as the subgenomic flavivirus RNA (sfRNA), is secreted into mosquito saliva within the extracellular vesicles and can facilitate infection of the acceptor human cells when delivered together with infectious virus in mosquito saliva. The study potentially expands our understanding of flavivirus transmission.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Divergent regulation of KCNQ1/E1 by targeted recruitment of protein kinase A to distinct sites on the channel complex

1. Xinle Zou
2. Sri Karthika Shanmugam
3. Scott A Kanner
4. Kevin J Sampson
5. Robert S Kass
6. Henry M Colecraft

• Curated by eLife

  eLife assessment

  This important study provides a substantial advance with a method by which a protein target resistant to therapeutic approaches can be uniquely modulated by a cellular protein kinase ferried by nanobodies to a precise molecular site of recruitment. Evidence for this major claim is compelling, but evidence for some of the minor claims seems incomplete. The work will be of broad interest to cell biologists, cardiovascular researchers, and drug developers.

Reviewed by eLife

Personalized Virus Load Curves of SARS-CoV-2 Infection

1. Thomas Hillen
2. Carlos Contreras
3. Jay M. Newby

Reviewed by ScreenIT

Obesity alters Ace2 and Tmprss2 expression in lung, trachea, and esophagus in a sex-dependent manner: Implications for COVID-19

1. Dylan C. Sarver
2. G. William Wong

Reviewed by ScreenIT

Accounting for body mass effects in the estimation of field metabolic rates from body acceleration

1. Evan E. Byrnes
2. Karissa O. Lear
3. Lauran R. Brewster
4. Nicholas M. Whitney
5. Matthew J. Smukall
6. Nicola J. Armstrong
7. Adrian C. Gleiss

Reviewed by ScreenIT

Quality of Life and Depressive Symptoms Among Peruvian University Students During the COVID-19 Pandemic

1. Joel Figueroa-Quiñones
2. Julio Cjuno
3. Daniel Machay-Pak
4. Miguel Ipanaqué-Zapata

Reviewed by ScreenIT

Exosomes from COVID-19 Patients Carry Tenascin-C and Fibrinogen-β in Triggering Inflammatory Signals in Cells of Distant Organ

1. Subhayan Sur
2. Mousumi Khatun
3. Robert Steele
4. T. Scott Isbell
5. Ranjit Ray
6. Ratna B. Ray

Reviewed by ScreenIT