Physiology

Placental uptake and metabolism of 25(OH)vitamin D determine its activity within the fetoplacental unit

1. Brogan Ashley
2. Claire Simner
3. Antigoni Manousopoulou
4. Carl Jenkinson
5. Felicity Hey
6. Jennifer M Frost
7. Faisal I Rezwan
8. Cory H White
9. Emma M Lofthouse
10. Emily Hyde
11. Laura DF Cooke
12. Sheila Barton
13. Pamela Mahon
14. Elizabeth M Curtis
15. Rebecca J Moon
16. Sarah R Crozier
17. Hazel M Inskip
18. Keith M Godfrey
19. John W Holloway
20. Cyrus Cooper
21. Kerry S Jones
22. Rohan M Lewis
23. Martin Hewison
24. Spiros DD Garbis
25. Miguel R Branco
26. Nicholas C Harvey
27. Jane K Cleal

• Curated by eLife

  Evaluation Summary:

  This manuscript uses primary placental units and villous explants to examine the placental metabolism of vitamin D and effects of vitamin D on placental gene expression. The studies demonstrate that the placenta actively transports 25D, such that the fetal levels are dependent on placental function rather than simple diffusion from the maternal circulation. Furthermore, they demonstrate actions of vitamin D on placental gene expression. This paper should be of interest to cell biologists and obstetricians/gynaecologists studying the role of the placenta in fetal growth and development.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

SOX4 facilitates PGR protein stability and FOXO1 expression conducive for human endometrial decidualization

1. Pinxiu Huang
2. Wenbo Deng
3. Haili Bao
4. Zhong Lin
5. Mengying Liu
6. Jinxiang Wu
7. Xiaobo Zhou
8. Manting Qiao
9. Yihua Yang
10. Han Cai
11. Faiza Rao
12. Jingsi Chen
13. Dunjin Chen
14. Jinhua Lu
15. Haibin Wang
16. Aiping Qin
17. Shuangbo Kong

• Curated by eLife

  Evaluation Summary:

  Huang et al have identified SOX4 as a key regulatory factor that controls prolactin and FOXO1 transcription factor which in turn regulate decidualization, an important process in embryo development. Further, they have identified that SOX4 also regulates progesterone receptor and dysregulated SOX4-progesterone and ubiquitin ligase HERC4can lead to embryo implantation failure thus explaining the molecular basis of recurrent implantation failure in humans. Overall the study is interesting and the data are very strong. Some concerns noted were the use of immortalized stromal cells, and incomplete nature of studies with human endometrial stroll cells from endometriosis patients, and lack of sufficient discussion in some parts of the text, and whether specific progesterone isofoms are involved downstream of SOX4.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

SARS-CoV-2 Spike Protein Regulation of Angiotensin Converting Enzyme 2 and Tissue Renin-Angiotensin Systems: Influence of Biologic Sex

1. Charles M. Ensor
2. Yasir AlSiraj
3. Robin Shoemaker
4. Jamie Sturgill
5. Suresh Keshavamurthy
6. Elizabeth M. Gordon
7. Brittany E. Dong
8. Christopher Waters
9. Lisa A Cassis

Reviewed by ScreenIT

Neural basis for regulation of vasopressin secretion by anticipated disturbances in osmolality

1. Angela Kim
2. Joseph C Madara
3. Chen Wu
4. Mark L Andermann
5. Bradford B Lowell

• Curated by eLife

  Evaluation Summary:

  This study investigates the important question of how vasopressin neurons, which are critical for fluid balance, are rapidly activated or inhibited when mice begin to eat or drink. The study presents useful anatomic data on connectivity between these neurons and other structures and tests a broad range of possible inputs that could mediate these effects. The conclusions are largely supported by the data.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Bi-phasic effect of gelatin in myogenesis and skeletal muscle regeneration

1. Xiaoling Liu
2. Er Zu
3. Xinyu Chang
4. Xiaowei Ma
5. Ziqi Wang
6. Xintong Song
7. Xiangru Li
8. Qing Yu
9. Ken-ichiro Kamei
10. Toshihiko Hayashi
11. Kazunori Mizuno
12. Shunji Hattori
13. Hitomi Fujisaki
14. Takashi Ikejima
15. Dan Ohtan Wang

Reviewed by Review Commons

Genetically engineered mice for combinatorial cardiovascular optobiology

1. Frank K Lee
2. Jane C Lee
3. Bo Shui
4. Shaun Reining
5. Megan Jibilian
6. David M Small
7. Jason S Jones
8. Nathaniel H Allan-Rahill
9. Michael RE Lamont
10. Megan A Rizzo
11. Sendoa Tajada
12. Manuel F Navedo
13. Luis Fernando Santana
14. Nozomi Nishimura
15. Michael I Kotlikoff

• Curated by eLife

  Evaluation Summary:

  The manuscript by Lee and co-workers describes the development of 21 unique transgenic mouse lines that express optogenetic sensors and effectors in a cell lineage-specific fashion. The knock-in approach allows the sensors and effectors to be rapidly combined or moved to different backgrounds, such as genetic disease models. Such manipulations are often impractical when using a Cre-based system. This constitutes a vital advantage for many studies. The new mice described here will be very powerful tools to study physiology and alteration in disease models.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Topography and motion of acid-sensing ion channel intracellular domains

1. Tyler Couch
2. Kyle D Berger
3. Dana L Kneisley
4. Tyler W McCullock
5. Paul Kammermeier
6. David M Maclean

• Curated by eLife

  Evaluation Summary:

  This is a rigorous and clearly written paper that provides quantitative data for the scope of intracellular signalling by ASIC channels. These channels are involved in pain signalling and other processes, and apparently can couple to intracellular pathways independent of ion flow. Here the authors measure the movements of the unstructured intracellular parts of ASIC using fluorescence spectroscopy coupled to functional measurements.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, #2, and #3 agreed to share their names with the authors.)

Reviewed by eLife

Fibrinogen αC-subregions critically contribute blood clot fibre growth, mechanical stability, and resistance to fibrinolysis

1. Helen R McPherson
2. Cedric Duval
3. Stephen R Baker
4. Matthew S Hindle
5. Lih T Cheah
6. Nathan L Asquith
7. Marco M Domingues
8. Victoria C Ridger
9. Simon DA Connell
10. Khalid M Naseem
11. Helen Philippou
12. Ramzi A Ajjan
13. Robert AS Ariëns

• Curated by eLife

  Evaluation Summary:

  This paper is of interest to a broad audience in the blood coagulation and fibrinolysis field. Previously undescribed roles in a range of blood clot properties are attributed to a region of the clotting protein fibrinogen, using state-of-the-art methodology. The data support the main conclusions of the paper, open new avenues of investigation for understanding clot properties, and have clinical implications.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Use of hiPSC-Derived Cardiomyocytes to Rule Out Proarrhythmic Effects of Drugs: The Case of Hydroxychloroquine in COVID-19

1. Luca Sala
2. Vladislav Leonov
3. Manuela Mura
4. Federica Giannetti
5. Aleksandr Khudiakov
6. Alessandra Moretti
7. Lia Crotti
8. Massimiliano Gnecchi
9. Peter J. Schwartz

Reviewed by ScreenIT

Modulation of fracture healing by the transient accumulation of senescent cells

1. Dominik Saul
2. David G Monroe
3. Jennifer L Rowsey
4. Robyn Laura Kosinsky
5. Stephanie J Vos
6. Madison L Doolittle
7. Joshua N Farr
8. Sundeep Khosla

• Curated by eLife

  Evaluation Summary:

  This is a well conducted study that examines the potential role of senescent cells in fracture healing. Furthermore, the authors have used senolytic agents to examine if targeting cellular senescence will have a significant impact on fracture healing phenotype. The manuscript is succinctly written, and the methods and results are well described. These data provide the first demonstration for a potential role for cellular senescence in regulating fracture healing. The authors’ conclusions are largely supported by the experimental data.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewers #1, #2, and #3 agreed to share their names with the authors.)

Reviewed by eLife