Obesity alters Ace2 and Tmprss2 expression in lung, trachea, and esophagus in a sex-dependent manner: Implications for COVID-19
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
No abstract available
Article activity feed
-
-
SciScore for 10.1101/2020.10.13.337907: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: All animal protocols were approved by the Institutional Animal Care and Use Committee of The Johns Hopkins University School of Medicine (Protocol # MO16M431). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Mice: C57BL/6J male and female mice were housed in polycarbonate cages on a 12-h light-dark photocycle with ad libitum access to water and food, with no more than five adult mice per cage. Table 2: Resources
Experimental Models: Organisms/Strains Sentences Resources Mice: C57BL/6J male and female mice were housed in polycarbonate cages on a 12-h light-dark photocycle with ad libitum access to water and … SciScore for 10.1101/2020.10.13.337907: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: All animal protocols were approved by the Institutional Animal Care and Use Committee of The Johns Hopkins University School of Medicine (Protocol # MO16M431). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Mice: C57BL/6J male and female mice were housed in polycarbonate cages on a 12-h light-dark photocycle with ad libitum access to water and food, with no more than five adult mice per cage. Table 2: Resources
Experimental Models: Organisms/Strains Sentences Resources Mice: C57BL/6J male and female mice were housed in polycarbonate cages on a 12-h light-dark photocycle with ad libitum access to water and food, with no more than five adult mice per cage. C57BL/6Jsuggested: NoneSoftware and Algorithms Sentences Resources Prism 8 (GraphPad Software, La Jolla, CA, USA) was used for statistical analyses, and differences were considered to be statistically significant at P < 0.05. GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Some limitations of the present study are noted, however. (1) Only Ace2 and Tmprss2 transcript levels were assessed, and we presumed that ACE2 and TMPRSS2 protein abundance correlated with their mRNA levels. (2) Quantifications of Ace2 and Tmprss2 transcript levels were performed on bulk RNA isolated from whole tissue; as such, we could not ascertain the cell source responsible for altered expression of Ace2 and Tmprss2 in the lung and trachea of obese mice. However, recent single-cell RNA sequencing analyses identified type II pneumocytes and ciliated cells as the two major cell types in the human lung that express ACE2 and TMPRSS2 [21–24]. (3) Ace2 and Tmprss2 expression levels were only assessed in ~6-month-old mice, corresponding to young human adults. COVID-19 mortality is significantly higher in people >70 years old [39]. Thus, further assessment of Ace2 and Tmprss2 expression in midlife (~1-year-old) and geriatric (~1.5-year-old) mice is warranted. In summary, our study provides valuable insights into dynamic expression of the SARS-CoV-2 cell entry receptor and an important associated protease in the context of obesity and sex. This information will help inform our ongoing understanding of COVID-19 pathophysiology.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
-
