Pathology

Clinical validation of innovative, low cost, kit-free, RNA processing protocol for RT-PCR based COVID-19 testing

1. Nikhil Shri Sahajpal
2. Ashis K Mondal
3. Allan Njau
4. Sudha Ananth
5. Arvind Kothandaraman
6. Madhuri Hegde
7. Alka Chaubey
8. Sandeep Padala
9. Vamsi Kota
10. Kevin Caspary
11. Stephen M Tompkins
12. Ted Ross
13. Amyn M. Rojani
14. Ravindra Kolhe

Reviewed by Rapid Reviews Infectious Diseases, ScreenIT

Targeting Leishmania donovani Sphingosine Kinase 1 using PF-543 enhances immune response and limits parasite load

1. Evanka Madan
2. Ruby Bansal
3. Jhalak Singhal
4. Monica Saini
5. Sadat Shafi
6. Nishant Joshi
7. Shailja Singh

Reviewed by Rapid Reviews Infectious Diseases

Infection of ratites with clade 2.3.4.4b HPAIV H5N1: Potential implications for zoonotic risk

1. Holly A. Coombes
2. Jacob Terrey
3. Audra-Lynne Schlachter
4. Phoebe McCarter
5. Isabella Regina
6. Richard Hepple
7. Natalie McGinn
8. James Seekings
9. Jayne Cooper
10. Benjamin Clifton
11. Benjamin C. Mollett
12. Marco Falchieri
13. Alejandro Nunez
14. Scott M. Reid
15. Joe James
16. Ashley C. Banyard

Reviewed by Rapid Reviews Infectious Diseases

Hepatocyte Circadian Clocks Control Cholesterol Metabolism and Protect From Metabolic Dysfunction-Associated Steatohepatitis (MASH)

1. Leonardo Vinicius Monteiro de Assis
2. Lina Jegodzinski
3. Julica Inderhees
4. Sylvia Wowro
5. Juliana Marques Affonso
6. Isabel Heyde
7. Emelie Luise Fischer
8. Witigo von Schönfels
9. Andrea Schenk
10. Florian Roßner
11. Michael Schupp
12. Jens U. Marquardt
13. Münevver Demir
14. Henrik Oster

Reviewed by PREreview

Hyperactivated Glycolysis Drives Spatially-Patterned Kupffer Cell Depletion in MASLD

1. Jia He
2. Ran Li
3. Cheng Xie
4. Xiane Zhu
5. Keqin Wang
6. Zhao Shan

• Curated by eLife

  eLife Assessment

  The authors aim to understand why Kupffer cells (KCs) die in metabolic-associated steatotic liver disease (MASLD). This is a useful study using in vitro studies and an in vivo genetic mouse model, suggesting that increased glycolysis contributes to KC death in MASLD. However, the data presented are incomplete as some inconsistencies in the results presented are identified in the characterisation of KCs. This work will be of interest to researchers in the immunology and metabolism fields.

Reviewed by eLife

Protective role of RIPK1 scaffolding against HDV-induced hepatocyte cell death and the significance of cytokines in mice

1. Gracián Camps
2. Sheila Maestro
3. Laura Torella
4. Diego Herrero
5. Carla Usai
6. Martin Bilbao-Arribas
7. Ana Aldaz
8. Cristina Olagüe
9. Africa Vales
10. Lester Suárez-Amarán
11. Rafael Aldabe
12. Gloria Gonzalez-Aseguinolaza

Reviewed by Rapid Reviews Infectious Diseases

R-Spondin Mimetic, SZN-043, Induced Proliferation and Expression of Wnt Target Genes, Two Impaired Features in Human Alcohol-Associated Liver Disease

1. Trevor Fisher
2. Mehaben Patel
3. Shalaka Deshmukh
4. Darshini Shah
5. Chenggang Lu
6. Maureen Newman
7. Jay Ye
8. Russell Fletcher
9. Geertrui F Vanhove
10. Jay Tibbitts
11. Yang Li
12. Nicholas J Skill
13. Zhihong Yang
14. Suthat Liangpunsakul
15. Helene Baribault

• Curated by eLife

  eLife Assessment

  This important study highlights the novel role of RSPO mimetic SZN-043 in the activation of hepatic WNT signaling and promoting hepatocyte regeneration. The authors provide convincing evidence of SZN-043 increasing hepatocytes proliferation in various mouse models, including a humanized mouse liver model, ALD model and CCL4 fibrosis model. This study will be of interest to researchers in liver regeneration and repair mechanisms.

Reviewed by eLife

Action mechanism of a novel agrichemical quinofumelin against Fusarium graminearum

1. Qian Xiu
2. Xiaoru Yin
3. Yuanyuan Chen
4. Ziyang Zhang
5. Yushuai Mao
6. Tianshi Wang
7. Jie Zhang
8. Mingguo Zhou
9. Yabing Duan

• Curated by eLife

  eLife Assessment

  In this valuable study, the authors show the physiological response and molecular pathway mediating the effect of quinofumelin, a developed fungicide with an unknown mechanism. The authors present convincing data suggesting the involvement of the uridine/uracil biosynthesis pathway, by combining in vivo microbiology characterization as well as in vitro biochemical binding results.

Reviewed by eLife

Telomeric antisense oligonucleotides reduce premature aging phenotypes in telomerase mutant zebrafish

1. Giulia Allavena
2. Francesca Rossiello
3. Aurora Irene Idilli
4. Marina Mione
5. Fabrizio d’Adda di Fagagna
6. Miguel Godinho Ferreira
7. Bruno Lopes-Bastos

Reviewed by preLights

The penetration ring is a novel infection structure formed by the penetration peg for invading plant cell membrane in rice blast fungus

1. Wenqin Fang
2. Xiaoyu Zai
3. Jia Chen
4. Yakubu Saddeeq Abubakar
5. Qiuqiu Wu
6. Zhenyu Fang
7. Xiuwei Huang
8. Xiang Gan
9. Daniel J Ebbole
10. Zonghua Wang
11. Wenhui Zheng

• Curated by eLife

  eLife Assessment

  This valuable study provides strong evidence for the development of a penetration ring during Magnaporthe oryzae infection and, supported by knockout and expression studies, shows that Ppe1 is involved in the virulence of the fungus. Although the authors demonstrated the close association of Ppe1 with the host plasma membrane, the work fell short in providing direct evidence for its role at the host-pathogen interface and the precise molecular function of the penetration ring. Therefore, the study presented strong structural and phenotypic characterization but remains incomplete regarding mechanistic insights of Ppe1.

Reviewed by eLife