Pathology

Telomeric antisense oligonucleotides reduce premature aging phenotypes in telomerase mutant zebrafish

1. Giulia Allavena
2. Francesca Rossiello
3. Aurora Irene Idilli
4. Marina Mione
5. Fabrizio d’Adda di Fagagna
6. Miguel Godinho Ferreira
7. Bruno Lopes-Bastos

Reviewed by preLights

The penetration ring is a novel infection structure formed by the penetration peg for invading plant cell membrane in rice blast fungus

1. Wenqin Fang
2. Xiaoyu Zai
3. Jia Chen
4. Yakubu Saddeeq Abubakar
5. Qiuqiu Wu
6. Zhenyu Fang
7. Xiuwei Huang
8. Xiang Gan
9. Daniel J Ebbole
10. Zonghua Wang
11. Wenhui Zheng

• Curated by eLife

  eLife Assessment

  This valuable study provides strong evidence for the development of a penetration ring during Magnaporthe oryzae infection and, supported by knockout and expression studies, shows that Ppe1 is involved in the virulence of the fungus. Although the authors demonstrated the close association of Ppe1 with the host plasma membrane, the work fell short in providing direct evidence for its role at the host-pathogen interface and the precise molecular function of the penetration ring. Therefore, the study presented strong structural and phenotypic characterization but remains incomplete regarding mechanistic insights of Ppe1.

Reviewed by eLife

mTOR inhibition in Q175 Huntington’s disease model mice facilitates neuronal autophagy and mutant huntingtin clearance

1. Philip Stavrides
2. Chris N Goulbourne
3. James Peddy
4. Chunfeng Huo
5. Mala Rao
6. Vinod Khetarpal
7. Deanna M Marchionini
8. Ralph A Nixon
9. Dun-Sheng Yang

• Curated by eLife

  eLife Assessment

  This study presents an important finding on the alterations in the autophagic-lysosomal pathway in a Huntington's disease model. The evidence supporting the claims of the authors is convincing. The original reviewers have found most of the issues previously raised have been addressed although further suggestions are given for consideration. These comments are listed below. The work will be of interest to neuroscientists working on HD.

Reviewed by eLife

Pathology of Influenza A (H5N1) infection in pinnipeds reveals novel tissue tropism and vertical transmission

1. Carla Fiorito
2. Ana Colom
3. Antonio Fernández
4. Paula Alonso-Almorox
5. Marisa Andrada
6. Daniel Lombardo
7. Eva Sierra

Reviewed by Rapid Reviews Infectious Diseases, PREreview

MicroRNA-26b protects against MASH development in mice and can be efficiently targeted with lipid nanoparticles

1. Linsey Peters
2. Leonida Rakateli
3. Rosanna Huchzermeier
4. Andrea Bonnin-Marquez
5. Sanne L Maas
6. Cheng Lin
7. Alexander Jans
8. Yana Geng
9. Alan Gorter
10. Marion Gijbels
11. Sander Rensen
12. Peter Olinga
13. Tim Hendrikx
14. Marcin Krawczyk
15. Malvina Brisbois
16. Joachim Jankowski
17. Kiril Bidzhekov
18. Christian Weber
19. Erik AL Biessen
20. Ronit Shiri-Sverdlov
21. Tom Houben
22. Yvonne Döring
23. Matthias Bartneck
24. Emiel van der Vorst

• Curated by eLife

  eLife Assessment

  This study presents valuable insights into the involvement of miR-26b in the progression of metabolic dysfunction-associated steatohepatitis (MASH). The delivery of microRNA-containing nanoparticles to reduce MASH severity has practical implications as a therapeutic strategy. The authors use two sets of transgenic mouse models, conducted kinase activity profiling of mouse liver samples, and supplemented their findings with additional experiments on human liver and plasma, providing solid support for their findings.

Reviewed by eLife

Dynamics of compartment-specific proteomic landscapes of hepatotoxic and cholestatic models of liver fibrosis

1. Marketa Jirouskova
2. Karel Harant
3. Pavel Cejnar
4. Srikant Ojha
5. Katerina Korelova
6. Lenka Sarnova
7. Eva Sticova
8. Christoph H Mayr
9. Herbert B Schiller
10. Martin Gregor

• Curated by eLife

  eLife Assessment

  This important study suggests that the composition of the extracellular matrix in a mouse model of liver fibrosis changes depending on the cause of liver fibrosis. The data could be used as a foundation for future antifibrotic therapies. The strength of evidence is convincing with respect to the use of animal models and proteomic analysis. The study provides a helpful inventory of proteins up or down-regulated.

Reviewed by eLife

Factor XI localization in human deep venous thrombus and function of activated factor XI on venous thrombus formation and hemostasis

1. Nobuyuki Oguri
2. Toshihiro Gi
3. Eriko Nakamura
4. Kazunari Maekawa
5. Eiji Furukoji
6. Hoshimi Okawa
7. Sho Kouyama
8. Saki Horiuchi
9. Akira Sawaguchi
10. Tatefumi Sakae
11. Minako Azuma
12. Yujiro Asada
13. Atsushi Yamashita

Reviewed by preLights

Kynurenine monooxygenase blockade reduces endometriosis-like lesions, improves visceral hyperalgesia, and rescues mice from a negative behavioural phenotype in experimental endometriosis

1. Ben Higgins
2. Ioannis Simitsidellis
3. Xiaozhong Zheng
4. Frances Collins
5. Natalie ZM Homer
6. Scott G Denham
7. Joanna P Simpson
8. Mike Millar
9. Lyndsey Boswell
10. Hee Y Lee
11. Yeon G Kim
12. Kyung H Park
13. Larry C Park
14. Patrick J Sweeney
15. Gerard Feraille
16. Alessandro Taddei
17. David Chagras
18. Thierry Alvarez
19. Scott P Webster
20. Andrew Horne
21. Philippa TK Saunders
22. Damian J Mole

• Curated by eLife

  eLife Assessment

  This useful study holds importance within the focused scope of endometriosis treatment, providing initial evidence of a potential new therapeutic target. The strength of the evidence is solid, as the methods, data, and analyses support the authors' conclusions regarding the specific aims. The study provides promising preliminary evidence of KMO implication in endometriosis, but it falls short of establishing a strong rationale for proposing KNS898 as a treatment for endometriosis given the limitations in evidence and mechanistic insights.

Reviewed by eLife

Enhancing bone regeneration and osseointegration using rhPTH(1-34) and dimeric R25CPTH(1-34) in an osteoporotic beagle model

1. Jeong-Oh Shin
2. Jong-Bin Lee
3. Sihoon Lee
4. Jin-Woo Kim

• Curated by eLife

  eLife Assessment

  Using a large animal model, this study demonstrated valuable findings that R25CPTH(1-34), based on a mutation associated with isolated familial hypoparathyroidism, generated an anabolic osteointegration effect comparable to that of native PTH1-34. The translational aspect of this human-to-animal work, aimed at animal-to-human translation for therapeutic purposes, should be highlighted. The study design is simple and straightforward, and the methods used are solid. The authors have addressed all the questions in their revision.

Reviewed by eLife

PKR activation-induced mitochondrial dysfunction in HIV-transgenic mice with nephropathy

1. Teruhiko Yoshida
2. Khun Zaw Latt
3. Avi Z Rosenberg
4. Briana A Santo
5. Komuraiah Myakala
6. Yu Ishimoto
7. Yongmei Zhao
8. Shashi Shrivastav
9. Bryce A Jones
10. Xiaoping Yang
11. Xiaoxin X Wang
12. Vincent M Tutino
13. Pinaki Sarder
14. Moshe Levi
15. Koji Okamoto
16. Cheryl A Winkler
17. Jeffrey B Kopp

• Curated by eLife

  eLife assessment

  This study presents valuable new insights into a HIV-associated nephropathy (HIVAN) kidney phenotype in the Tg26 transgenic mouse model, and delineates the kidney cell types that express HIV genes and are injured in these HIV-transgenic mice. A series of compelling experiments demonstrated that PKR inhibition can ameliorate HIVAN with reversal of mitochondrial dysfunction (mainly confined to endothelial cells), a prominent feature shared in other kidney diseases. The data support that inhibition of PKR and mitochondrial dysfunction has potential clinical significance for HIVAN.

Reviewed by eLife