Neuroscience

A temporally restricted function of the dopamine receptor Dop1R2 during memory formation

1. Jenifer C Kaldun
2. Emanuele Calia
3. Ganesh Chinmai Bangalore Mukunda
4. Cornelia Fritsch
5. Nikita Komarov
6. Simon G Sprecher

• Curated by eLife

  eLife Assessment

  The authors design and implement an elegant strategy to delete genomic sequences encoding the dopamine receptor dop1R2 from specific subsets of mushroom body neurons (ab, a'b' and gamma) and show that while none of these manipulations affect short term appetitive or aversive memory, loss of dop1R2 from ab or a'b' block the ability of flies to display measurable forms of longer forms of memory. These findings are important in confirming and extending prior observations, and well supported by convincing evidence that build on precise techniques for genetic perturbation.

Reviewed by eLife

Acute aerobic exercise intensity does not modulate pain potentially due to differences in fitness levels and sex effects – results from a pharmacological fMRI study

1. Janne I Nold
2. Tahmine Fadai
3. Christian Büchel

• Curated by eLife

  eLife Assessment

  In this valuable study, Nold et al. examined exercise-induced pain modulation in a pharmacological within-subject fMRI study using the opioid-antagonist naloxone and different levels of aerobic exercise intensity and pain. This investigation provides solid evidence to show that the intensity of exercise does not seem to impact the hypoalgesic effect. Moreover, exploratory analysis identified that fitness level and sex may potentially play a role in exercise-induced hypoalgesia, and that further confirmatory studies are required in order to verify these findings.

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Midbrain somatostatin-expressing cells control pain-suppression during defensive states

1. Nanci Winke
2. Frank Aby
3. Daniel Jercog
4. Thomas Bienvenu
5. Coline Riffault
6. Rabia Bouali-Benazzouz
7. Juliette Viellard
8. Delphine Girard
9. Zoé Grivet
10. Marc Landry
11. Laia Castell
12. Emmanuel Valjent
13. Stephane Valerio
14. Pascal Fossat
15. Cyril Herry

• Curated by eLife

  eLife Assessment

  The study is a timely and important contribution to our knowledge of the circuit mechanisms of fear analgesia. The novel cue-induced analgesia paradigm allowed a compelling identification of a brainstem circuit element, i.e., somatostatin-expressing neurons within the ventrolateral periaqueductal grey that project to the rostroventral medulla, in mediating fear analgesia. The vlPAG is a known region of pain modulation, and this study adds key insight to the circuit involved in fear-associated analgesia. This work will be of interest to systems and behavioral neuroscientists, especially those interested in emotional behavior, pain, and/or brainstem function.

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Ultraslow serotonin oscillations in the hippocampus delineate substates across NREM and waking

1. Claire Cooper
2. Daniel Parthier
3. Jeremie Sibille
4. John J Tukker
5. Nicolas Tritsch
6. Dietmar Schmitz

• Curated by eLife

  eLife Assessment

  This important study demonstrates that slow fluctuations in serotonin release during wakefulness and non-REM sleep correspond to periods of heightened arousal or enhanced offline information processing. The evidence supporting this claim is convincing, and the methodology is robust and broadly applicable, likely to benefit many researchers in the field. This work will be of significant interest to neuroscientists studying sleep, memory, and neuromodulation.

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Inference technique for the synaptic conductances in rhythmically active networks and application to respiratory central pattern generation circuits

1. Yaroslav Molkov
2. Anke Borgmann
3. Hidehiko Koizumi
4. Noriyuki Hama
5. Ruli Zhang
6. Jeffrey Smith

• Curated by eLife

  eLife Assessment

  This work describes an inference technique for extracting information about relative contributions of excitatory and inhibitory synaptic drive onto single neurons in neural networks. The electrophysiological techniques and results are of high quality, and the analytical work is novel and potentially powerful, yet with several untested assumptions underlying the approach. This is nevertheless solid work that will be valuable to neuroscience labs interested in exploring alternative approaches to studies of integrated synaptic connectivity.

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Genome-wide consensus transcriptional signatures identify synaptic pruning linking Alzheimer’s disease and epilepsy

1. Huihong Li
2. Zhiran Xie
3. Yuxuan Tian
4. Ruoyin Zhou
5. Yaxi Yang
6. Bingying Lin
7. Si Chen
8. Jie Wu
9. Zihan Deng
10. Jianwei Li
11. Mingjie Chen
12. Xueke Liu
13. Yushan Sun
14. Bing Huang
15. Naili Wei
16. Xiaoyu Ji

Reviewed by PREreview

Uncovering the electrical synapse proteome in retinal neurons via in vivo proximity labeling

1. Stephan Tetenborg
2. Eyad Shihabeddin
3. Elizebeth Olive Akansha Manoj Kumar
4. Crystal L Sigulinsky
5. Karin Dedek
6. Ya-Ping Lin
7. Fabio A Echeverry
8. Hannah Hoff
9. Alberto E Pereda
10. Bryan W Jones
11. Christophe P Ribelayga
12. Klaus Ebnet
13. Ken Matsuura
14. John O’Brien

• Curated by eLife

  eLife Assessment

  This is an important study that characterized proteins associated with electrical synapses in zebrafish and mouse retinal neurons using proximity labeling approaches, complemented by biochemical and histological validations. The resulting protein interactome datasets are convincing and reveal novel scaffold proteins at the electrical synapse. Additional quantification and validation would strengthen the work further.

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Progressive remote memory decline coincides with parvalbumin interneuron hyperexcitability and enhanced inhibition of cortical engram cells in a mouse model of Alzheimer’s disease

1. Julia J. van Adrichem
2. Rolinka J. van der Loo
3. Romina Ambrosini Defendi
4. August B. Smit
5. Michel C. van den Oever
6. Ronald E. van Kesteren

• Curated by eLife

  eLife Assessment

  This valuable study explores changes in remote memory impairment in an amyloid pathology mouse model, demonstrating that progressive deficits coincide with inhibitory interneuron alterations. While the findings shed light on circuit remodeling in this model, the mechanistic links between heightened inhibition and memory loss are currently incomplete. Additional data and deeper analysis may be needed to fully substantiate the authors' interpretations.

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SpikeMAP: An unsupervised pipeline for the identification of cortical excitatory and inhibitory neurons in high-density multielectrode arrays with ground-truth validation

1. E. Giraud
2. M. Lynn
3. P. Vincent-Lamarre
4. J-C. Béïque
5. J-P. Thivierge

• Curated by eLife

  eLife Assessment

  In this manuscript, the authors describe a software package for automatic differentiation of action potentials generated by excitatory and inhibitory neurons, acquired using high-density microelectrode arrays. The work is valuable as it offers a tool with the potential to automatically identify these neuron types in vitro. However, it is incomplete due to limited comparison with ground truth data from optogenetically identified interneuron subtypes and with existing spike sorting pipelines available to users.

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Ultrastructural sublaminar-specific diversity of excitatory synaptic boutons in layer 1 of the adult human temporal lobe neocortex

1. Astrid Rollenhagen
2. Akram Sadeghi
3. Bernd Walkenfort
4. Claus C Hilgetag
5. Kurt Sätzler
6. Joachim HR Lübke

• Curated by eLife

  eLife Assessment

  This study provides important information on the ultrastructural organization of layer 1 of the human neocortex. The quantitative assessment of various synaptic parameters, astrocytic coverage and mitochondrial morphology is based on convincing experimental approaches. These data provide new information on the detailed morphology of human neocortical tissue that will be of interest to neuroscientists working on different network functions.

Reviewed by eLife