Molecular Biology

Transcriptomic meta-analysis in plaque psoriasis: an integrative bioinformatic approach to deciphering the genetic landscape and molecular pathways

1. Teresa Torres-Moral
2. Josep Riera-Monroig
3. Gemma Tell-Martí
4. Jaume Bagué
5. José F. Català-Senent
6. Francisco J. Roig
7. Míriam Potrony
8. Francisco García-García
9. Susana Puig

Reviewed by Arcadia Science

Small-molecule activation of TFEB alleviates Niemann–Pick disease type C via promoting lysosomal exocytosis and biogenesis

1. Kaili Du
2. Hongyu Chen
3. Zhaonan Pan
4. Mengli Zhao
5. Shixue Cheng
6. Yu Luo
7. Wenhe Zhang
8. Dan Li

• Curated by eLife

  eLife Assessment

  This study reports that activation of TFEB promotes lysosomal exocytosis and clearance of cholesterol from lysosomes, the strength of evidence for which is convincing with appropriate and validated methodology in line with current state-of-the-art. The significance of the findings is important in the context of Niemann-Pick Disease Type C as well as other subfields.

Reviewed by eLife

Changes in local mineral homeostasis facilitate the formation of benign and malignant testicular microcalcifications

1. Ida Marie Boisen
2. Nadia Krarup Knudsen
3. John E Nielsen
4. Ireen Kooij
5. Mathilde Louise Bagger
6. Jovanna Kaludjerovic
7. Peter O'Shaughnessy
8. Peter W Andrews
9. Noriko Ide
10. Birgitte G Toft
11. Anders Juul
12. Arnela Mehmedbasic
13. Anne Jørgensen
14. Lee B Smith
15. Richard Norman
16. Ewa Rajpert-De Meyts
17. Beate Lanske
18. Martin Blomberg Jensen

• Curated by eLife

  eLife Assessment

  This valuable study reports the link between a disruption in testicular mineral (phosphate) homeostasis, FGF23 expression, and Sertoli cell dysfunction. The data supporting the conclusion are solid. This work will be of interest to biomedical researchers working on testis biology and male infertility. The assessment is based on the editors' critical evaluation of the authors' responses.

Reviewed by eLife

Ubiquitin Ligase ITCH Regulates Life Cycle of SARS-CoV-2 Virus

1. Qiwang Xiang
2. Camille Wouters
3. Peixi Chang
4. Yu-Ning Lu
5. Mingming Liu
6. Haocheng Wang
7. Junqin Yang
8. Andrew Pekosz
9. Yanjin Zhang
10. Jiou Wang

• Curated by eLife

  eLife Assessment

  This study highlights ITCH as a regulator of SARS-CoV-2 replication by promoting K63-linked ubiquitination of M and E proteins. While the findings are potentially useful, the approaches are overly reliant on ectopic expression models and lack direct mechanistic evidence that ubiquitination of M and E has functional relevance. Accordingly, the strength of evidence is incomplete, as further experiments are needed to validate the findings and address potential confounding factors.

Reviewed by eLife

ORMDL3 restrains type I interferon signaling and anti-tumor immunity by promoting RIG-I degradation

1. Qi Zeng
2. Chen Yao
3. Shimeng Zhang
4. Yizhi Mao
5. Jing Wang
6. Ziyang Wang
7. Chunjie Sheng
8. Shuai Chen

• Curated by eLife

  eLife Assessment

  This study provides important insights into the regulation of type-I interferon signaling and anti-tumor immunity, demonstrating that ORMDL3 promotes RIG-I degradation to suppress immune responses. The evidence is convincing, with well-executed mechanistic experiments and in vivo validation in syngeneic tumor models. These findings have significant implications for cancer immunotherapy, highlighting ORMDL3 as a potential therapeutic target.

Reviewed by eLife

Insufficiency of 40S ribosomal proteins, RPS26 and RPS25, negatively affects biosynthesis of polyglycine-containing proteins in fragile-X associated conditions

1. Katarzyna Tutak
2. Izabela Broniarek
3. Andrzej Zielezinski
4. Daria Niewiadomska
5. Tomasz Skrzypczak
6. Anna Baud
7. Krzysztof Sobczak

• Curated by eLife

  eLife Assessment

  In this valuable study, Tutak and colleagues set out to identify factors that mediate Repeat Associated Non-AUG (RAN) translation of CGG repeats in the FMR1 mRNA which are implicated in toxic protein accumulation that underpins ensuing neurological pathologies. The authors provide solid evidence that RPS26 may be implicated in mediating the RAN translation of FMR1 mRNA. This article should be of broad interest to researchers in the variety of disciplines including post-transcriptional regulation of gene expression and neurobiology.

Reviewed by eLife

Maf-family bZIP transcription factor NRL interacts with RNA-binding proteins and R-loops in retinal photoreceptors

1. Ximena Corso Diaz
2. Xulong Liang
3. Kiam Preston
4. Bilguun Tegshee
5. Milton A English
6. Jacob Nellissery
7. Sharda Prasad Yadav
8. Claire Marchal
9. Anand Swaroop

• Curated by eLife

  eLife Assessment

  This important study employed multiple orthogonal techniques and tissue samples to investigate the interaction between the NRL transcription factor and RNA-binding proteins in the retina. The findings are convincing to support an interaction between NRL and the DHX9 helicase. The significance of the study could be enhanced with functional experiments of NRL-R-loop interactions in the developing retina and their potential role in photoreceptor health and gene regulation.

Reviewed by eLife

The SUMO activating enzyme subunit, SAE2, contributes SUMO protein bias for mitotic fidelity

1. Alexandra K. Walker
2. Alexander J. Lanz
3. Mohammed Jamshad
4. Alexander J. Garvin
5. Peter Wotherspoon
6. Benjamin F. Cooper
7. Timothy J. Knowles
8. Joanna R. Morris

Reviewed by Review Commons

Improved base editing and functional screening in Leishmania via co-expression of the AsCas12a ultra variant, a T7 RNA polymerase, and a cytosine base editor

1. Nicole Herrmann May
2. Anh Cao
3. Annika Schmid
4. Fabian Link
5. Jorge Arias-del-Angel
6. Elisabeth Meiser
7. Tom Beneke

• Curated by eLife

  eLife Assessment

  This important article describes a meticulously-developed improved strategy for generation of functionally null mutants in Leishmania spp. via cytosine base editing, with reduced background toxicity and enhanced efficiency relative to a previously-described method. The authors show use of the strategy in a small-scale loss-of-function screen, providing compelling evidence that large-scale screens will be possible. The newly developed tools will be of great interest to researchers working with Leishmania and beyond.

Reviewed by eLife

Embryological cellular origins and hypoxia-mediated mechanisms in PIK3CA -Driven refractory vascular malformations

1. Sota Torii
2. Keiki Nagaharu
3. Nanako Nakanishi
4. Hidehito Usui
5. Yumiko Hori
6. Katsutoshi Hirose
7. Satoru Toyosawa
8. Eiichi Morii
9. Mitsunaga Narushima
10. Yoshiaki Kubota
11. Osamu Nakagawa
12. Kyoko Imanaka-Yoshida
13. Kazuaki Maruyama

Reviewed by Review Commons