Molecular Biology

A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses

1. Joep Beumer
2. Maarten H. Geurts
3. Mart M. Lamers
4. Jens Puschhof
5. Jingshu Zhang
6. Jelte van der Vaart
7. Anna Z. Mykytyn
8. Tim I. Breugem
9. Samra Riesebosch
10. Debby Schipper
11. Petra B. van den Doel
12. Wim de Lau
13. Cayetano Pleguezuelos-Manzano
14. Georg Busslinger
15. Bart L. Haagmans
16. Hans Clevers

Reviewed by ScreenIT

Quantitative Control of Noise in Mammalian Gene Expression by Dynamic Histone Regulations

1. Deng Tan
2. Rui Chen
3. Yuejian Mo
4. Wei Xu
5. Xibin Lu
6. Huiyu He
7. Shu Gu
8. Fan Jiang
9. Weimin Fan
10. Yilin Wang
11. Xi Chen
12. Wei Huang

• Curated by eLife

  Evaluation Summary:

  This paper will be of interest to biologists who study mechanisms of cell-to-cell variability in gene expression and those who wish to have a tool to alter variability in mammalian cells. Key regulators of gene expression variability in mammalian cells are identified and noise modulation in a synthetic system is shown. The data quality is high. A model for the origin of the observed noise is proposed, but will require some additional experimental evidence.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

First description of two immune escape indian B.1.1.420 and B.1.617.1 SARS-CoV2 variants in France

1. Vincent Garcia
2. Véronique Vig
3. Laurent Peillard
4. Alaa Ramdani
5. Sofiane Mohamed
6. Philippe Halfon

Reviewed by ScreenIT

Structural basis for cell-type specific evolution of viral fitness by SARS-CoV-2

1. Kapil Gupta
2. Christine Toelzer
3. Maia Kavanagh Williamson
4. Deborah K. Shoemark
5. A. Sofia F. Oliveira
6. David A. Matthews
7. Abdulaziz Almuqrin
8. Oskar Staufer
9. Sathish K.N. Yadav
10. Ufuk Borucu
11. Frederic Garzoni
12. Daniel Fitzgerald
13. Joachim Spatz
14. Adrian J. Mulholland
15. Andrew D. Davidson
16. Christiane Schaffitzel
17. Imre Berger

Reviewed by ScreenIT

Design of the SARS-CoV-2 RBD vaccine antigen improves neutralizing antibody response

1. Thayne H. Dickey
2. Wai Kwan Tang
3. Brandi Butler
4. Tarik Ouahes
5. Sachy Orr-Gonzalez
6. Nichole D. Salinas
7. Lynn E. Lambert
8. Niraj H. Tolia

Reviewed by ScreenIT

SARS-CoV-2 variant B.1.617 is resistant to bamlanivimab and evades antibodies induced by infection and vaccination

1. Markus Hoffmann
2. Heike Hofmann-Winkler
3. Nadine Krüger
4. Amy Kempf
5. Inga Nehlmeier
6. Luise Graichen
7. Prerna Arora
8. Anzhalika Sidarovich
9. Anna-Sophie Moldenhauer
10. Martin S. Winkler
11. Sebastian Schulz
12. Hans-Martin Jäck
13. Metodi V. Stankov
14. Georg M.N. Behrens
15. Stefan Pöhlmann

Reviewed by Rapid Reviews Infectious Diseases, ScreenIT

Emerging genetic diversity of SARS-CoV-2 RNA dependent RNA polymerase (RdRp) alters its B-cell epitopes

1. Sushant Kumar
2. Gajendra Kumar Azad

Reviewed by ScreenIT

¹ H, ¹³ C and ¹⁵ N resonance assignment of the SARS-CoV-2 full-length nsp1 protein and its mutants reveals its unique secondary structure features in solution

1. Tatiana Agback
2. Francisco Dominguez
3. Ilya Frolov
4. Elena I. Frolova
5. Peter Agback

Reviewed by ScreenIT

The molecular basis of coupling between poly(A)-tail length and translational efficiency

1. Kehui Xiang
2. David P Bartel

• Curated by eLife

  Evaluation Summary:

  This manuscript addresses a long-standing question, namely how does the poly(A) tail influence translational efficiency? It will therefore be of broad interest to readers from many areas of molecular biology including those interested in translation, mRNA stability, development and gene expression in general. The authors convincingly set out three criteria that must be met for coupling of poly(A) tail length with translation.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Natural variants in SARS-CoV-2 S protein pinpoint structural and functional hotspots: implications for prophylaxis and therapeutic strategies

1. Suman Pokhrel
2. Benjamin R. Kraemer
3. Scott Burkholz
4. Daria Mochly-Rosen

Reviewed by ScreenIT