Receptor-binding domain recombinant protein on alum-CpG induces broad protection against SARS-CoV-2 variants of concern

Article activity feed

1. Version published to 10.1016/j.vaccine.2022.05.007

   Jun 1, 2022
2. 

   Version published to 10.1101/2021.07.06.451353v2 on bioRxiv

   Mar 22, 2022
3. 

   ScreenIT

   Jul 10, 2021

   SciScore for 10.1101/2021.07.06.451353: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IACUC: Preclinical Design: All animal experiments were performed under a Baylor College of Medicine Institutional Animal Care and Use Committee approved protocol.
   Sex as a biological variable	Female BALB/c mice, age 11-13 weeks old, were immunized two (2) times intramuscularly at 21-day intervals with formulations shown in Table 1, and then euthanized two (2) weeks after the second vaccinations.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Pseudovirions were generated by transfection of 293T cells as we previously described [14].	293T suggested: CCLV Cat# CCLV-RIE …

   SciScore for 10.1101/2021.07.06.451353: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IACUC: Preclinical Design: All animal experiments were performed under a Baylor College of Medicine Institutional Animal Care and Use Committee approved protocol.
   Sex as a biological variable	Female BALB/c mice, age 11-13 weeks old, were immunized two (2) times intramuscularly at 21-day intervals with formulations shown in Table 1, and then euthanized two (2) weeks after the second vaccinations.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Pseudovirions were generated by transfection of 293T cells as we previously described [14].	293T suggested: CCLV Cat# CCLV-RIE 1018, RRID:CVCL_0063)
   Next, 100 µL of sera-pseudovirus were added to 293T-hACE2 cells in 96-well poly-D-lysine coated culture plates.	293T-hACE2 suggested: None
   Experimental Models: Organisms/Strains
   Sentences	Resources
   Female BALB/c mice, age 11-13 weeks old, were immunized two (2) times intramuscularly at 21-day intervals with formulations shown in Table 1, and then euthanized two (2) weeks after the second vaccinations.	BALB/c suggested: None
   Recombinant DNA
   Sentences	Resources
   The plasmids used for the pseudovirus production are the luciferase-encoding reporter plasmid (pNL4-3.lucR-E-, [24]), Gag/Pol-encoding packaging construct (pΔ8.9, [25]), and codon-optimized SARS-CoV-2 spike protein expression plasmids (pcDNA3.1-CoV-2 S gene) based on clone p278-1 [26].	pNL4-3.lucR-E- suggested: None pcDNA3.1-CoV-2 suggested: None
   Software and Algorithms
   Sentences	Resources
   All data were plotted in Graphpad Prism 6 as individual samples and the geometric mean.	Graphpad Prism suggested: (GraphPad Prism, RRID:SCR_002798)
   Raw data was analyzed using Bio-Plex Manager software, and further analysis was done with Excel and Prism.	Bio-Plex Manager suggested: None Excel suggested: None Prism suggested: (PRISM, RRID:SCR_005375)

   ---

   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

   ---

   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

   ---

   Results from TrialIdentifier: No clinical trial numbers were referenced.

   ---

   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

   ---

   Results from JetFighter: We did not find any issues relating to colormaps.

   ---

   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

   ---

   Results from scite Reference Check: We found no unreliable references.

   ---

   Read the original source
4. 

   Version published to 10.1101/2021.07.06.451353v1 on bioRxiv

   Jul 7, 2021