Molecular Biology

Irradiation of UVC LED at 277 nm inactivates coronaviruses by photodegradation of spike protein

1. Qunxiang Ong
2. J. W. Ronnie Teo
3. Joshua Dela Cruz
4. Elijah Wee
5. Winson Wee
6. Weiping Han

Reviewed by ScreenIT

MINERVA: A Facile Strategy for SARS-CoV-2 Whole-Genome Deep Sequencing of Clinical Samples

1. Chen Chen
2. Jizhou Li
3. Lin Di
4. Qiuyu Jing
5. Pengcheng Du
6. Chuan Song
7. Jiarui Li
8. Qiong Li
9. Yunlong Cao
10. X. Sunney Xie
11. Angela R. Wu
12. Hui Zeng
13. Yanyi Huang
14. Jianbin Wang

Reviewed by ScreenIT, preLights

A twin UGUA motif directs the balance between gene isoforms through CFIm and the mTORC1 signaling pathway

1. R Samuel Herron
2. Alexander K Kunisky
3. Jessica R Madden
4. Vivian I Anyaeche
5. May Z Maung
6. Hun-Way Hwang

• Curated by eLife

  eLife assessment

  In this valuable report, the authors explore the connection between mTORC signaling and APA regulated by the CFIm complex. Using a combination of genetic and functional genomics approaches, the study reveals that a twin UGUA motif is a potent cis acting regulation of PAS usage that is recognized by CFIm. Overall, the evidence in general is convincing and supports the conclusions and provides the field with additional mechanistic insight of how signaling connects to APA regulatory machinery.

Reviewed by eLife

The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting

1. Matthias M. Zimmer
2. Anuja Kibe
3. Ulfert Rand
4. Lukas Pekarek
5. Liqing Ye
6. Stefan Buck
7. Redmond P. Smyth
8. Luka Cicin-Sain
9. Neva Caliskan

Reviewed by ScreenIT

The N-terminal domain of SARS-CoV-2 nsp1 plays key roles in suppression of cellular gene expression and preservation of viral gene expression

1. Aaron S. Mendez
2. Michael Ly
3. Angélica M. González-Sánchez
4. Ella Hartenian
5. Nicholas T. Ingolia
6. Jamie H. Cate
7. Britt A. Glaunsinger

Reviewed by ScreenIT

Long non-coding RNA Neat1 and paraspeckle components are translational regulators in hypoxia

1. Anne-Claire Godet
2. Emilie Roussel
3. Florian David
4. Fransky Hantelys
5. Florent Morfoisse
6. Joffrey Alves
7. Françoise Pujol
8. Isabelle Ader
9. Edouard Bertrand
10. Odile Burlet-Schiltz
11. Carine Froment
12. Anthony K Henras
13. Patrice Vitali
14. Eric Lacazette
15. Florence Tatin
16. Barbara Garmy-Susini
17. Anne-Catherine Prats

• Curated by eLife

  Evaluation Summary:

  This study reports a novel link by which specific cellular mRNAs, that contain internal ribosome sites (IRES), are made competent for translation in paraspeckles in the nucleus. The data showed that a long noncoding RNA, Neat1, is the major player to add transacting factors to the internal ribosome entry site located in fibroblast growth factor 1 mRNAs in the nucleus. This event occurs during hypoxia in mouse cardiomyocytes and is, thus, relevant to gene expression during angiogenesis.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Dimerization of SARS-CoV-2 nucleocapsid protein affects sensitivity of ELISA based diagnostics of COVID-19

1. Wajihul Hasan Khan
2. Nida Khan
3. Avinash Mishra
4. Surbhi Gupta
5. Vikrant Bansode
6. Deepa Mehta
7. Rahul Bhambure
8. M. Ahmad Ansari
9. Shukla Das
10. Anurag S. Rathore

Reviewed by ScreenIT

A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses

1. Joep Beumer
2. Maarten H. Geurts
3. Mart M. Lamers
4. Jens Puschhof
5. Jingshu Zhang
6. Jelte van der Vaart
7. Anna Z. Mykytyn
8. Tim I. Breugem
9. Samra Riesebosch
10. Debby Schipper
11. Petra B. van den Doel
12. Wim de Lau
13. Cayetano Pleguezuelos-Manzano
14. Georg Busslinger
15. Bart L. Haagmans
16. Hans Clevers

Reviewed by ScreenIT

Quantitative control of noise in mammalian gene expression by dynamic histone regulation

1. Deng Tan
2. Rui Chen
3. Yuejian Mo
4. Shu Gu
5. Jiao Ma
6. Wei Xu
7. Xibin Lu
8. Huiyu He
9. Fan Jiang
10. Weimin Fan
11. Yili Wang
12. Xi Chen
13. Wei Huang

• Curated by eLife

  Evaluation Summary:

  This paper will be of interest to biologists who study mechanisms of cell-to-cell variability in gene expression and those who wish to have a tool to alter variability in mammalian cells. Key regulators of gene expression variability in mammalian cells are identified and noise modulation in a synthetic system is shown. The data quality is high. A model for the origin of the observed noise is proposed, but will require some additional experimental evidence.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

First description of two immune escape indian B.1.1.420 and B.1.617.1 SARS-CoV2 variants in France

1. Vincent Garcia
2. Véronique Vig
3. Laurent Peillard
4. Alaa Ramdani
5. Sofiane Mohamed
6. Philippe Halfon

Reviewed by ScreenIT