Molecular Biology

Establishment of developmental gene silencing by ordered polycomb complex recruitment in early zebrafish embryos

1. Graham JM Hickey
2. Candice L Wike
3. Xichen Nie
4. Yixuan Guo
5. Mengyao Tan
6. Patrick J Murphy
7. Bradley R Cairns

• Curated by eLife

  Evaluation Summary:

  This manuscript is of broad interest to developmental biologists and those studying transcriptional/epigenetic regulation of cell-specific and housekeeping gene programs. The work demonstrates that Polycomb complexes coordinate the regulation of distinct groups of genes during early embryogenesis, which offers interesting insights into how very early embryos differentially control housekeeping versus specific developmental gene promoters/enhancers. The data are of high quality, and the conclusions are insightful yet measured.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Genome-Wide Characterization of SARS-CoV-2 Cytopathogenic Proteins in the Search of Antiviral Targets

1. Jiantao Zhang
2. Qi Li
3. Ruth S. Cruz Cosme
4. Volodymyr Gerzanich
5. Qiyi Tang
6. J. Marc Simard
7. Richard Y. Zhao

Reviewed by ScreenIT

A DCL3 dicing code within Pol IV-RDR2 transcripts diversifies the siRNA pool guiding RNA-directed DNA methylation

1. Andrew Loffer
2. Jasleen Singh
3. Akihito Fukudome
4. Vibhor Mishra
5. Feng Wang
6. Craig S Pikaard

• Curated by eLife

  Evaluation Summary:

  The paper is of interest to RNA biologists, especially to those who study small RNAs. The findings deepen our understanding of the rules of DCL3 dicing and explain how 23-nt and 24-nt siRNAs in the RNA-directed DNA methylation pathway are produced. Overall, the data are of high quality and support the paper's conclusions.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Viral E protein neutralizes BET protein-mediated post-entry antagonism of SARS-CoV-2

1. Irene P. Chen
2. James E. Longbotham
3. Sarah McMahon
4. Rahul K. Suryawanshi
5. Mir M. Khalid
6. Taha Y. Taha
7. Takako Tabata
8. Jennifer M. Hayashi
9. Frank W. Soveg
10. Jared Carlson-Stevermer
11. Meghna Gupta
12. Meng Yao Zhang
13. Victor L. Lam
14. Yang Li
15. Zanlin Yu
16. Erron W. Titus
17. Amy Diallo
18. Jennifer Oki
19. Kevin Holden
20. Nevan Krogan
21. Danica Galonić Fujimori
22. Melanie Ott

Reviewed by ScreenIT

Profiling RT-LAMP tolerance of sequence variation for SARS-CoV-2 RNA detection

1. Esta Tamanaha
2. Yinhua Zhang
3. Nathan A. Tanner

Reviewed by ScreenIT

A panel of nanobodies recognizing conserved hidden clefts of all SARS-CoV-2 spike variants including Omicron

1. Ryota Maeda
2. Junso Fujita
3. Yoshinobu Konishi
4. Yasuhiro Kazuma
5. Hiroyuki Yamazaki
6. Itsuki Anzai
7. Tokiko Watanabe
8. Keishi Yamaguchi
9. Kazuki Kasai
10. Kayoko Nagata
11. Yutaro Yamaoka
12. Kei Miyakawa
13. Akihide Ryo
14. Kotaro Shirakawa
15. Kei Sato
16. Fumiaki Makino
17. Yoshiharu Matsuura
18. Tsuyoshi Inoue
19. Akihiro Imura
20. Keiichi Namba
21. Akifumi Takaori-Kondo

Reviewed by ScreenIT

Structure–function analysis of the nsp14 N7–guanine methyltransferase reveals an essential role in Betacoronavirus replication

1. Natacha S. Ogando
2. Priscila El Kazzi
3. Jessika C. Zevenhoven-Dobbe
4. Brenda W. Bontes
5. Alice Decombe
6. Clara C. Posthuma
7. Volker Thiel
8. Bruno Canard
9. François Ferron
10. Etienne Decroly
11. Eric J. Snijder

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Structural and functional characterization of NEMO cleavage by SARS-CoV-2 3CLpro

1. Mikhail A. Hameedi
2. Erica T. Prates
3. Michael R. Garvin
4. Irimpan I. Mathews
5. B. Kirtley Amos
6. Omar Demerdash
7. Mark Bechthold
8. Mamta Iyer
9. Simin Rahighi
10. Daniel W. Kneller
11. Andrey Kovalevsky
12. Stephan Irle
13. Van-Quan Vuong
14. Julie C. Mitchell
15. Audrey Labbe
16. Stephanie Galanie
17. Soichi Wakatsuki
18. Daniel Jacobson

Reviewed by ScreenIT

SARS-CoV-2 RdRp uses NDPs as a substrate and is able to incorporate NHC into RNA from diphosphate form molnupiravir

1. Maofeng Wang
2. Cancan Wu
3. Nan Liu
4. Fengyu Zhang
5. Hongjie Dong
6. Shuai Wang
7. Min Chen
8. Xiaoqiong Jiang
9. Kundi Zhang
10. Lichuan Gu

Reviewed by ScreenIT

Molecular signature of postmortem lung tissue from COVID-19 patients suggests distinct trajectories driving mortality

1. Anshul Budhraja
2. Anubhav Basu
3. Atish Gheware
4. Dasari Abhilash
5. Seesandra Rajagopala
6. Suman Pakala
7. Madhuresh Sumit
8. Animesh Ray
9. Arulselvi Subramaniam
10. Purva Mathur
11. Aruna Nambirajan
12. Sachin Kumar
13. Ritu Gupta
14. Naveet Wig
15. Anjan Trikha
16. Randeep Guleria
17. Chitra Sarkar
18. Ishaan Gupta
19. Deepali Jain

Reviewed by ScreenIT