Molecular Biology

SARS-CoV-2 Nsp14 mediates the effects of viral infection on the host cell transcriptome

1. Michela Zaffagni
2. Jenna M Harris
3. Ines L Patop
4. Nagarjuna Reddy Pamudurti
5. Sinead Nguyen
6. Sebastian Kadener

• Curated by eLife

  Evaluation Summary:

  The paper makes the provocative claim that SARS-CoV-2 Nsp14 is the key protein that mediates the effects of viral infection on the host cell transcriptome. The current evidence for this claim is good, but the paper would benefit from a few additional experiments. If confirmed by these experiments, the conclusion is unexpected and important, especially in these COVID times.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife, ScreenIT

circFL-seq reveals full-length circular RNAs with rolling circular reverse transcription and nanopore sequencing

1. Zelin Liu
2. Changyu Tao
3. Shiwei Li
4. Minghao Du
5. Yongtai Bai
6. Xueyan Hu
7. Yu Li
8. Jian Chen
9. Ence Yang

• Curated by eLife

  Evaluation Summary:

  This study provides an experimental and computational method for the identification and reconstruction of full-length circRNAs using nanopore sequencing,. With a better comparison to other existing methods in the field and a clearer demonstration of the advantages of the described methodology, the work would be of great interest to researchers in the circular RNA community.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

N4-hydroxycytidine and inhibitors of dihydroorotate dehydrogenase synergistically suppress SARS-CoV-2 replication

1. Kim M. Stegmann
2. Antje Dickmanns
3. Natalie Heinen
4. Uwe Groß
5. Dirk Görlich
6. Stephanie Pfaender
7. Matthias Dobbelstein

Reviewed by ScreenIT

Mutations within the Open Reading Frame (ORF) including Ochre stop codon of the Surface Glycoprotein gene of SARS-CoV-2 virus erase potential seed location motifs of human non-coding microRNAs

1. Krishna Himmatbhai Goyani
2. Shalin Vaniawala
3. Pratap Narayan Mukhopadhyaya

Reviewed by ScreenIT

Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants

1. Jason Z. Zhang
2. Hsien-Wei Yeh
3. Alexandra C. Walls
4. Basile I. M. Wicky
5. Kaitlin R. Sprouse
6. Laura A. VanBlargan
7. Rebecca Treger
8. Alfredo Quijano-Rubio
9. Minh N. Pham
10. John C. Kraft
11. Ian C. Haydon
12. Wei Yang
13. Michelle DeWitt
14. John E. Bowen
15. Cameron M. Chow
16. Lauren Carter
17. Rashmi Ravichandran
18. Mark H. Wener
19. Lance Stewart
20. David Veesler
21. Michael S. Diamond
22. Alexander L. Greninger
23. David M. Koelle
24. David Baker

Reviewed by ScreenIT

Increased lung cell entry of B.1.617.2 and evasion of antibodies induced by infection and BNT162b2 vaccination

1. Prerna Arora
2. Amy Kempf
3. Inga Nehlmeier
4. Anzhalika Sidarovich
5. Nadine Krüger
6. Luise Graichen
7. Anna-Sophie Moldenhauer
8. Martin S. Winkler
9. Sebastian Schulz
10. Hans-Martin Jäck
11. Metodi V. Stankov
12. Georg M. N. Behrens
13. Stefan Pöhlmann
14. Markus Hoffmann

Reviewed by ScreenIT

Influence of viral transport media and freeze-thaw cycling on the sensitivity of qRT-PCR detection of SARS-CoV-2 nucleic acids

1. Cian Holohan
2. Sophia Hanarahan
3. Nathan Feely
4. Peng Li
5. John O’Connell
6. Catherine Moss
7. Michael Carr
8. Oya Tagit
9. Gil U Lee

Reviewed by ScreenIT

Molecular Dynamics Analysis of Fast-Spreading Severe Acute Respiratory Syndrome Coronavirus 2 Variants and Their Effects on the Interaction with Human Angiotensin-Converting Enzyme 2

1. Anacleto Silva de Souza
2. Vitor Martins de Freitas Amorim
3. Gabriela D. A. Guardia
4. Felipe R. C. dos Santos
5. Filipe F. dos Santos
6. Robson Francisco de Souza
7. Guilherme de Araujo Juvenal
8. Yihua Huang
9. Pingju Ge
10. Yinan Jiang
11. Coco Li
12. Prajwal Paudel
13. Henning Ulrich
14. Pedro A. F. Galante
15. Cristiane Rodrigues Guzzo

Reviewed by ScreenIT

Double-stranded RNA drives SARS-CoV-2 nucleocapsid protein to undergo phase separation at specific temperatures

1. Christine A Roden
2. Yifan Dai
3. Catherine A Giannetti
4. Ian Seim
5. Myungwoon Lee
6. Rachel Sealfon
7. Grace A McLaughlin
8. Mark A Boerneke
9. Christiane Iserman
10. Samuel A Wey
11. Joanne L Ekena
12. Olga G Troyanskaya
13. Kevin M Weeks
14. Lingchong You
15. Ashutosh Chilkoti
16. Amy S Gladfelter

Reviewed by ScreenIT

In vitro evaluation of the effect of mutations in primer binding sites on detection of SARS-CoV-2 by RT-qPCR

1. Fee Zimmermann
2. Maria Urban
3. Christian Krüger
4. Mathias Walter
5. Roman Wölfel
6. Katrin Zwirglmaier

Reviewed by ScreenIT