Molecular Biology

Discovery and functional interrogation of SARS-CoV-2 protein-RNA interactions

1. Joy S. Xiang
2. Jasmine R. Mueller
3. En-Ching Luo
4. Brian A. Yee
5. Danielle Schafer
6. Jonathan C. Schmok
7. Frederick E. Tan
8. Katherine Rothamel
9. Rachael N. McVicar
10. Elizabeth M. Kwong
11. Krysten L. Jones
12. Hsuan-Lin Her
13. Chun-Yuan Chen
14. Anthony Q. Vu
15. Wenhao Jin
16. Samuel S. Park
17. Phuong Le
18. Kristopher W. Brannan
19. Eric R. Kofman
20. Yanhua Li
21. Alexandra T. Tankka
22. Kevin D. Dong
23. Yan Song
24. Aaron F. Carlin
25. Eric L. Van Nostrand
26. Sandra L. Leibel
27. Gene W. Yeo

Reviewed by ScreenIT

Targeted Down Regulation Of Core Mitochondrial Genes During SARS-CoV-2 Infection

1. Joseph W. Guarnieri
2. Joseph M. Dybas
3. Hossein Fazelinia
4. Man S. Kim
5. Justin Frere
6. Yuanchao Zhang
7. Yentli Soto Albrecht
8. Deborah G. Murdock
9. Alessia Angelin
10. Larry N. Singh
11. Scott L. Weiss
12. Sonja M. Best
13. Marie T. Lott
14. Henry Cope
15. Viktorija Zaksas
16. Amanda Saravia-Butler
17. Cem Meydan
18. Jonathan Foox
19. Christopher Mozsary
20. Yared H. Kidane
21. Waldemar Priebe
22. Mark R. Emmett
23. Robert Meller
24. Urminder Singh
25. Yaron Bram
26. Benjamin R. tenOever
27. Mark T. Heise
28. Nathaniel J. Moorman
29. Emily A. Madden
30. Sharon A. Taft-Benz
31. Elizabeth J. Anderson
32. Wes A. Sanders
33. Rebekah J. Dickmander
34. Victoria K. Baxter
35. Stephen B. Baylin
36. Eve Syrkin Wurtele
37. Pedro M. Moraes-Vieira
38. Deanne Taylor
39. Christopher E. Mason
40. Jonathan C. Schisler
41. Robert E. Schwartz
42. Afshin Beheshti
43. Douglas C. Wallace

Reviewed by ScreenIT

Genomic characterization of SARS-CoV-2 from Islamabad, Pakistan by Rapid Nanopore sequencing

1. Nazish Badar
2. Aamer Ikram
3. Muhammad Salman
4. Massab Umair
5. Zaira Rehman
6. Abdul Ahad
7. Hamza Ahmed Mirza
8. Masroor Alam
9. Nayab mehmood
10. Uzma Bashir

Reviewed by ScreenIT

Inhibiting USP16 rescues stem cell aging and memory in an Alzheimer’s model

1. Felicia Reinitz
2. Elizabeth Y Chen
3. Benedetta Nicolis di Robilant
4. Bayarsaikhan Chuluun
5. Jane Antony
6. Robert C Jones
7. Neha Gubbi
8. Karen Lee
9. William Hai Dang Ho
10. Sai Saroja Kolluru
11. Dalong Qian
12. Maddalena Adorno
13. Katja Piltti
14. Aileen Anderson
15. Michelle Monje
16. H Craig Heller
17. Stephen R Quake
18. Michael F Clarke

• Curated by eLife

  Evaluation Summary:

  This is interesting and potentially impactful work uncovering mechanisms of stem cell exhaustion and impaired self-renewal that precede inflammatory phenotypes in Alzheimer's disease. The authors describe a mechanism converging on BMP signaling that may represent an early therapeutic target. The reviewers of this manuscript were excited about the potential impact but had some concerns which, if addressed, will strengthen the conclusions reached in this work.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Omicron Spike Protein Has a Positive Electrostatic Surface That Promotes ACE2 Recognition and Antibody Escape

1. Hin Hark Gan
2. John Zinno
3. Fabio Piano
4. Kristin C. Gunsalus

Reviewed by ScreenIT

SARS-CoV-2 Nsp1 N-terminal and linker regions as a platform for host translational shutoff

1. Andrea Graziadei
2. Fabian Schildhauer
3. Christian Spahn
4. Matthew Kraushar
5. Juri Rappsilber

Reviewed by ScreenIT

An efficient and cost-effective method for directed mutagenesis at multiple dispersed sites—a case study with Omicron Spike DNA

1. Rita Rani
2. Kishore V L Parsa
3. Kiranam Chatti
4. Aarti Sevilimedu

Reviewed by ScreenIT

Epigenetic reprogramming by TET enzymes impacts co-transcriptional R-loops

1. João C Sabino
2. Madalena R de Almeida
3. Patrícia L Abreu
4. Ana M Ferreira
5. Paulo Caldas
6. Marco M Domingues
7. Nuno C Santos
8. Claus M Azzalin
9. Ana Rita Grosso
10. Sérgio Fernandes de Almeida

• Curated by eLife

  Evaluation Summary:

  The work provides new insight into the potential role of 5hmc DNA in specific transcriptional processes. This implies that 5hmC containing DNA has specific epigenetic features beyond being a simple intermediate in interconversion between repressive 5mC and active C DNA. This work has the merit to focus the attention on a rare DNA modification, helping defining its functions, starting from in vitro evidence and extending these findings in cellular context. There are some weaknesses in the presentation of the data, the controls and the statistical analyses.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Aerosol formation during processing of potentially infectious samples on Roche immunochemistry analyzers (cobas e analyzers) and in an end-to-end laboratory workflow to model SARS-CoV-2 infection risk for laboratory operators

1. Géza V. Burghardt
2. Markus Eckl
3. Doris Huether
4. Oliver H. D. Larbolette
5. Alessia Lo Faso
6. Beatus R. Ofenloch-Haehnle
7. Marlene A. Riesch
8. Rolf A. Herb

Reviewed by ScreenIT

How concerning is a SARS-CoV-2 variant of concern? Computational predictions and the variants labeling system

1. Dana Ashoor
2. Maryam Marzouq
3. Khaled Trabelsi
4. Sadok Chlif
5. Nasser Abotalib
6. Noureddine Ben Khalaf
7. Ahmed R. Ramadan
8. M-Dahmani Fathallah

Reviewed by ScreenIT