Molecular Biology

Hepatic MIR20B promotes nonalcoholic fatty liver disease by suppressing PPARA

1. Yo Han Lee
2. Hyun-Jun Jang
3. Sounkou Kim
4. Sun Sil Choi
5. Keon Woo Khim
6. Hye-jin Eom
7. Jimin Hyun
8. Kyeong Jin Shin
9. Young Chan Chae
10. Hongtae Kim
11. Jiyoung Park
12. Neung Hwa Park
13. Chang-Yun Woo
14. Chung Hwan Hong
15. Eun Hee Koh
16. Dougu Nam
17. Jang Hyun Choi

• Curated by eLife

  Evaluation Summary:

  The manuscript by Lee et al. provides mechanistic insight into the regulatory role of micro RNAs in modulating nuclear receptor expression and function. This is likely to have a high impact on the field as nuclear receptor regulation of metabolic disease is well established, however, the molecular mechanisms governing this process still remains unknown largely. Lee et al.'s manuscript provides a molecular target (miR-20b) that holds therapeutic potential in improving hepatic steatosis.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Critical Negatively Charged Residues Are Important for the Activity of SARS-CoV-1 and SARS-CoV-2 Fusion Peptides

1. Alex L. Lai
2. Jack H. Freed

Reviewed by ScreenIT

Predictions of the SARS-CoV-2 Omicron Variant (B.1.1.529) Spike Protein Receptor-Binding Domain Structure and Neutralizing Antibody Interactions

1. Colby T. Ford
2. Denis Jacob Machado
3. Daniel A. Janies

Reviewed by ScreenIT

SARS-CoV-2 Delta and delta derivatives impact on neutralization of Covishield recipient sera

1. Rima R. Sahay
2. Deepak Y. Patil
3. Gajanan N. Sapkal
4. Gururaj R. Deshpande
5. Anita M. Shete
6. Dimpal A. Nyayanit
7. Sanjay Kumar
8. Pragya D. Yadav

Reviewed by ScreenIT

Enhanced transcriptional heterogeneity mediated by NF-κB super-enhancers

1. Johannes N. Wibisana
2. Takehiko Inaba
3. Hisaaki Shinohara
4. Noriko Yumoto
5. Tetsutaro Hayashi
6. Mana Umeda
7. Masashi Ebisawa
8. Itoshi Nikaido
9. Yasushi Sako
10. Mariko Okada

Reviewed by Review Commons

Case Study of High-Throughput Drug Screening and Remote Data Collection for SARS-CoV-2 Main Protease by Using Serial Femtosecond X-ray Crystallography

1. Omur Guven
2. Mehmet Gul
3. Esra Ayan
4. J Austin Johnson
5. Baris Cakilkaya
6. Gozde Usta
7. Fatma Betul Ertem
8. Nurettin Tokay
9. Busra Yuksel
10. Oktay Gocenler
11. Cengizhan Buyukdag
12. Sabine Botha
13. Gihan Ketawala
14. Zhen Su
15. Brandon Hayes
16. Frederic Poitevin
17. Alexander Batyuk
18. Chun Hong Yoon
19. Christopher Kupitz
20. Serdar Durdagi
21. Raymond G. Sierra
22. Hasan DeMirci

Reviewed by ScreenIT

Protective Immunity of the Primary SARS-CoV-2 Infection Reduces Disease Severity Post Re-Infection with Delta Variants in Syrian Hamsters

1. Sreelekshmy Mohandas
2. Pragya D. Yadav
3. Anita Shete
4. Dimpal Nyayanit
5. Rajlaxmi Jain
6. Gajanan Sapkal
7. Chandrashekhar Mote

Reviewed by ScreenIT

DNA-PK promotes DNA end resection at DNA double strand breaks in G0 cells

1. Faith C Fowler
2. Bo-Ruei Chen
3. Nicholas Zolnerowich
4. Wei Wu
5. Raphael Pavani
6. Jacob Paiano
7. Chelsea Peart
8. Zulong Chen
9. André Nussenzweig
10. Barry P Sleckman
11. Jessica K Tyler

• Curated by eLife

  Evaluation Summary:

  This manuscript will be of interest for scientists interested in cell cycle, DNA repair, and genome stability reporting the unexpected discovery that the DNA-dependent protein kinase (DNA-PK) is required for DSB resection in G0 cells, whereas it is known and confirmed here that it inhibits resection in G1 and G2 cells. This finding has important implications for the clinical application of DNA-PK-targeted inhibitors. The data are of high quality and derive from two independent cell lines, genetic requirements were mostly established by gene knockouts, and the latest genome-wide sequencing techniques were applied to measure resection tracts. The key claims of the manuscript are supported by the data presented by the authors; however, further validations are needed to strengthen the quality and impact of the paper.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

A helicase-tethered ORC flip enables bidirectional helicase loading

1. Shalini Gupta
2. Larry J Friedman
3. Jeff Gelles
4. Stephen P Bell

• Curated by eLife

  Evaluation Summary:

  The paper describes single-molecule experiments that address the assembly of a double hexamer of the Mcm2-7 complex that is required to license all origins of DNA replication in eukaryotic cells by formation of a pre-Replicative Complex (pre-RC). The observations show that one Origin Recognition Complex, an ATP-dependent DNA binding protein, can load both Mcm2-7 hexamers in opposite orientation. The results nicely complement prior data on the mechanism of pre-RC assembly.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Establishment of developmental gene silencing by ordered polycomb complex recruitment in early zebrafish embryos

1. Graham JM Hickey
2. Candice L Wike
3. Xichen Nie
4. Yixuan Guo
5. Mengyao Tan
6. Patrick J Murphy
7. Bradley R Cairns

• Curated by eLife

  Evaluation Summary:

  This manuscript is of broad interest to developmental biologists and those studying transcriptional/epigenetic regulation of cell-specific and housekeeping gene programs. The work demonstrates that Polycomb complexes coordinate the regulation of distinct groups of genes during early embryogenesis, which offers interesting insights into how very early embryos differentially control housekeeping versus specific developmental gene promoters/enhancers. The data are of high quality, and the conclusions are insightful yet measured.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife