Molecular Biology

End-of-life targeted degradation of DAF-2 insulin/IGF-1 receptor promotes longevity free from growth-related pathologies

1. Richard Venz
2. Tina Pekec
3. Iskra Katic
4. Rafal Ciosk
5. Collin Yvès Ewald

• Curated by eLife

  Evaluation Summary:

  This paper reports careful work using auxin-mediated degradation to manipulate the DAF-2 insulin/IGF-1 receptor in specific tissues and at different ages of the nematode C. elegans. Since its initial discovery as a gene that could dramatically alter lifespan in this organism, daf-2 has been extensively studied. The authors make excellent and thorough use of their novel reagents to successfully add important new findings to our understanding of this broadly conserved aging pathway, including a finer dissection of the spatial and temporal requirements for DAF-2 in multiple processes, such as the decision window for entering dauer arrest and that altering the levels of this protein very late in life can still have dramatic effects on lifespan. Overall the data are convincing, but the presentation and clarity of the text could be improved.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Algorithm for the Quantitation of Variants of Concern for Rationally Designed Vaccines Based on the Isolation of SARS-CoV-2 Hawaiʻi Lineage B.1.243

1. David P. Maison
2. Lauren L. Ching
3. Sean B. Cleveland
4. Alanna C. Tseng
5. Eileen Nakano
6. Cecilia M. Shikuma
7. Vivek R. Nerurkar

Reviewed by ScreenIT

Cap-independent translation and a precisely located RNA sequence enable SARS-CoV-2 to control host translation and escape anti-viral response

1. Boris Slobodin
2. Urmila Sehrawat
3. Anastasia Lev
4. Daniel Hayat
5. Binyamin Zuckerman
6. Davide Fraticelli
7. Ariel Ogran
8. Amir Ben-Shmuel
9. Elad Bar-David
10. Haim Levy
11. Igor Ulitsky
12. Rivka Dikstein

Reviewed by ScreenIT

Ssl2/TFIIH function in transcription start site scanning by RNA polymerase II in Saccharomyces cerevisiae

1. Tingting Zhao
2. Irina O Vvedenskaya
3. William KM Lai
4. Shrabani Basu
5. B Franklin Pugh
6. Bryce E Nickels
7. Craig D Kaplan

• Curated by eLife

  Evaluation Summary:

  Kaplan and colleagues build upon their earlier work using genetic phenotypes to find and analyze mutations that determine how mRNA start sites are chosen. Here they provide convincing genetic evidence supporting a model in which the Transcription Factor II H (TFIIH) protein complex pushes downstream DNA back into the RNA polymerase active site, creating a window within which the polymerase can choose particular start sites. This will primarily interest those in the transcription field who are thinking about initiation mechanisms.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

SARS-CoV-2 Delta Variant Pathogenesis and Host Response in Syrian Hamsters

1. Sreelekshmy Mohandas
2. Pragya Dhruv Yadav
3. Anita Shete
4. Dimpal Nyayanit
5. Gajanan Sapkal
6. Kavita Lole
7. Nivedita Gupta

Reviewed by ScreenIT

Site-specific recognition of SARS-CoV-2 nsp1 protein with a tailored titanium dioxide nanoparticle – elucidation of the complex structure using NMR data and theoretical calculation

1. Peter Agback
2. Tatiana Agback
3. Francisco Dominguez
4. Elena I. Frolova
5. Gulaim A. Seisenbaeva
6. Vadim G. Kessler

Reviewed by ScreenIT

Improving RT-LAMP detection of SARS-CoV-2 RNA through primer set selection and combination

1. Yinhua Zhang
2. Nathan A. Tanner

Reviewed by ScreenIT

Comparable neutralization of SARS-CoV-2 Delta AY.1 and Delta with individuals sera vaccinated with BBV152

1. Pragya D Yadav
2. Rima R Sahay
3. Gajanan Sapkal
4. Dimpal Nyayanit
5. Anita M Shete
6. Gururaj Deshpande
7. Deepak Y Patil
8. Nivedita Gupta
9. Sanjay Kumar
10. Priya Abraham
11. Samiran Panda
12. Balram Bhargava

Reviewed by ScreenIT

SARS-CoV-2 Fears Green: The Chlorophyll Catabolite Pheophorbide A Is a Potent Antiviral

1. Guillermo H. Jimenez-Aleman
2. Victoria Castro
3. Addis Londaitsbehere
4. Marta Gutierrez-Rodríguez
5. Urtzi Garaigorta
6. Roberto Solano
7. Pablo Gastaminza

Reviewed by ScreenIT

Defining Proximity Proteome of Histone Modifications by Antibody-Mediated Protein A-APEX2 Labeling

1. Xinran Li
2. Jiaqi Zhou
3. Wenjuan Zhao
4. Qing Wen
5. Weijie Wang
6. Huipai Peng
7. Yuan Gao
8. Kelly J. Bouchonville
9. Steven M. Offer
10. Kuiming Chan
11. Zhiquan Wang
12. Nan Li
13. Haiyun Gan

Reviewed by Review Commons