Molecular Biology

Specialization of ubiquitin ligases to distinct nucleic acid sensors

1. Ibrahim Syed
2. Sheng Chen
3. David J. Peeler
4. Paul F. McKay
5. Marco A. Briones-Orta
6. Jennifer A. Bohn
7. Robin J. Shattock
8. Daniel Gonçalves-Carneiro

Reviewed by Review Commons

The chromatin remodeller CHD4 regulates transcription factor binding to both prevent activation of silent enhancers and maintain active regulatory elements

1. Andria Koulle
2. Oluwaseun Ogundele
3. Devina Shah
4. India Baker
5. Maya Lopez
6. David Lando
7. Nicola Reynolds
8. Ramy Ragheb
9. Ernest D Laue
10. Brian Hendrich

• Curated by eLife

  eLife Assessment

  This work offers important insights into the protein CHD4's function in chromatin remodeling and gene regulation in embryonic stem cells, supported by extensive biochemical, genomic, and imaging data. The use of an inducible degron system allows precise functional analysis, and the datasets generated represent a key resource for the field. The revised study offers compelling evidence and makes a significant contribution to understanding CHD4's role in epigenetic regulation. This work will be of interest to the epigenetics and stem biology fields.

Reviewed by eLife

A novel high-throughput single-cell DNA sequencing method reveals hidden genomic heterogeneity in the unicellular eukaryote Leishmania

1. Gabriel H Negreira
2. Pieter Monsieurs
3. Jean-Claude Dujardin
4. Malgorzata A Domagalska

• Curated by eLife

  eLife Assessment

  This important study, which tackles the challenge of analyzing genome integrity and instability in unicellular pathogens by introducing a novel single-cell genomics approach, presents compelling evidence that this new tool outperforms standard whole-genome amplification techniques. While thorough and rigorous, the work's impact would increase by providing scripts and data, as well as a description of the biological relevance that would make this method more appealing to the broad community studying genetic heterogeneity in diverse organisms.

Reviewed by eLife

HP1β and H3K9me3 Regulate Olfactory Receptor Choice and Transcriptional Identity

1. Martín Escamilla-del-Arenal
2. Rachel Duffié
3. Hani Shayya
4. Valentina Loconte
5. Axel Ekman
6. Lena Annika Street
7. Adan Horta
8. Daniele Canzio
9. Kevin Monahan
10. Carolyn Larabell
11. Marko Jovanovic
12. Stavros Lomvardas

Reviewed by PREreview

Translational response to mitochondrial stresses is orchestrated by tRNA modifications

1. Sherif Rashad
2. Shadi Al-Mesitef
3. Abdulrahman Mousa
4. Yuan Zhou
5. Daisuke Ando
6. Guangxin Sun
7. Tomoko Fukuuchi
8. Yuko Iwasaki
9. Jingdong Xiang
10. Shane R Byrne
11. Jingjing Sun
12. Masamitsu Maekawa
13. Daisuke Saigusa
14. Thomas J Begley
15. Peter C Dedon
16. Kuniyasu Niizuma

Reviewed by PREreview

Development of Loop Mediated Isothermal Amplification for Rapid and Sensitive Detection of Enteroaggregative Escherichia coli (EAEC)

1. Alazar Amare Amdiyee
2. Tesfaye Sisay Tessema

Reviewed by PREreview

Orphan nuclear receptors recruit TRIM28 to promote telomeric H3K9me3 for the alternative lengthening of telomeres pathway

1. Chia-Tsen Tsai
2. Venus Marie Gaela
3. Hsuan-Yu Hsia
4. Yu-Chen Huang
5. Liuh-Yow Chen

Reviewed by Review Commons

Competition for the conserved branch point sequence influences physiological outcomes in pre-mRNA splicing

1. Karen Larissa Pereira de Castro
2. Jose M Abril
3. Kuo-Chieh Liao
4. Haiping Hao
5. John Paul Donohue
6. William K Russell
7. W Samuel Fagg

• Curated by eLife

  eLife Assessment

  This important manuscript provides insights into the competition between Splicing Factor 1 (SF1) and Quaking (QKI) for binding at the ACUAA branch point sequence in a model intron, regulating exon inclusion. The study employs convincing, rigorous transcriptomic, proteomic, and reporter assays, with both mammalian cell culture and yeast models.

Reviewed by eLife

Role of ADMA-histones in dual-strand piRNA source loci recognition by Rhino

1. Raku Saito
2. Hirotsugu Ishizu
3. Ritsuko Harigai
4. Kensaku Murano
5. Yurika Namba
6. Mikiko C Siomi

• Curated by eLife

  eLife Assessment

  This valuable study identifies asymmetric dimethylarginine (ADMA) modification of histones as a potential key determinant of the initial genomic binding of Rhino, a Drosophila-specific chromatin protein essential for piRNA cluster specification. The authors provide correlative genomic and imaging data to support their model, although functional validation of the proposed mechanism remains incomplete. Testing the redundancy between dART4 and dART1, which together could affect the prominent piRNA loci, in addition to the minor ones investigated in the manuscript, may change our assessment.

Reviewed by eLife

Opposing CTCF and GATA4 activities set the pace of chromatin topology remodeling during cardiomyogenesis

1. Silvia Becca
2. Sara Bianchi
3. Elisa M. Hahn
4. Kirsten E. Snijders
5. Lukasz Truszkowski
6. Anna Krepelova
7. Francesco Neri
8. Davide Cacchiarelli
9. Sasha Mendjan
10. Elisa Balmas
11. Alessandro Bertero

Reviewed by Review Commons