Molecular Biology

On the path to reference genomes for all biodiversity: lessons learned and laboratory protocols created in the Sanger Tree of Life core laboratory over the first 2000 species

1. Caroline Howard
2. Amy Denton
3. Benjamin Jackson
4. Adam Bates
5. Jessie Jay
6. Halyna Yatsenko
7. Priyanka Sethu Raman
8. Abitha Thomas
9. Graeme Oatley
10. Raquel Vionette do Amaral
11. Zeynep Ene Göktan
12. Juan Pablo Narváez Gómez
13. Isabelle Clayton Lucey
14. Elizabeth Sinclair
15. Michael A. Quail
16. Mark Blaxter
17. Kerstin Howe
18. Mara K. N. Lawniczak

Reviewed by GigaScience

CryoDataBot: a pipeline to curate cryoEM datasets for AI-driven structural biology

1. Qibo Xu
2. Leon Wu
3. Michael Rebelo
4. Shi Feng
5. Xinye Yu
6. Farhanaz Farheen
7. Daisuke Kihara
8. Z. Hong Zhou

Reviewed by GigaScience

Herbivorous insects independently evolved salivary effectors to regulate plant immunity by destabilizing the malectin-LRR RLP NtRLP4

1. Xin Wang
2. Jia-Bao Lu
3. Yi-Zhe Wang
4. Xu-Hong Zhou
5. Jian-Ping Chen
6. Chuan-Xi Zhang
7. Jun-Min Li
8. Hai-Jian Huang

• Curated by eLife

  eLife Assessment

  This study provides an important contribution by showing that whiteflies and planthoppers use salivary effectors to suppress plant immunity through the receptor-like protein RLP4, suggesting convergent evolution in these insect lineages. The topic is of clear interest for understanding plant-insect interactions and offers ideas that could stimulate further research in the field. However, the strength of evidence is incomplete, as some aspects of the data and experimental design limit the extent to which the main claims are fully supported.

Reviewed by eLife

Endogenous Real Time Imaging Reveals Dynamic Chromosomal Mobility During Ligand-Mediated Transcriptional Burst Events

1. Susan Wang
2. Thomas Suter
3. Amir Gamliel
4. Yeeun Kim
5. Sreejith J Nair
6. Soohwan Oh
7. Feng Yang
8. Kenneth A Ohgi
9. Tobias Wagner
10. Steven Gan
11. Michael G Rosenfeld

• Curated by eLife

  eLife Assessment

  This manuscript presents significant and important work that advances single-molecule imaging technology of transcription with simultaneous analysis of several parameters. However, currently, the evidence is incomplete and requires further quantitation/description of the technologies used, further controls, and additional analysis of the data by other methods.

Reviewed by eLife

Fibro-Adipogenic Progenitors require autocrine IGF-I in homeostatic and regenerating skeletal muscle

1. Yangyi E. Luo
2. Zoe Abe-Teh
3. Tarek Y. Alsaghir
4. Li-Ying Kuo
5. Fahong Yu
6. Bradley E. Stoker
7. Ambili Bai Appu
8. Yumei Zhou
9. Feng Yue
10. Daniel Kopinke
11. Elisabeth R. Barton

Reviewed by preLights

Bora Bridges Aurora-A Activation and Substrate Recognition of PLK1

1. Jennifer A Miles
2. Matthew Batchelor
3. Martin Walko
4. Vanda Gunning
5. Andrew J Wilson
6. Megan H Wright
7. Richard Bayliss

Reviewed by Review Commons

Cohesin reconstitution and homologous recombination repair of DNA double-strand breaks in late mitosis

1. Jessel Ayra Plasencia
2. Sara Medina-Suárez
3. Esperanza Hernández-Carralero
4. Jonay García-Luis
5. Lorraine S Symington
6. Félix Machín

• Curated by eLife

  eLife Assessment

  This study provides convincing evidence that homologous recombination can occur in telophase-arrested cells, independently of cohesin subunits Smc 1-3. These findings are valuable as they point to investigate the role of cohesins re-association with chromatin in the allelic inter-sister repair by homologous recombination.

Reviewed by eLife

TRF2 Non-Telomeric Function is Indispensable for Neural stemness

1. Soujanya Vinayagamurthy
2. Amit Kumar Bhatt
3. Sulochana Bagri
4. Supratim Ghosh
5. Arpan Parichha
6. Arindam Maitra
7. Shantanu Chowdhury

Reviewed by Review Commons

Epigenetic delineation of the earliest cardiac lineage segregation by single-cell multi-omics

1. Peng Xie
2. Xu Jiang
3. Jingjing He
4. Qingyun Pan
5. Xianfa Yang
6. Yanying Zheng
7. Zhuanzhuan Che
8. Wenli Fan
9. Chen Wu
10. Weiheng Zheng
11. Shuhan Si
12. Kun Gao
13. Shiqi Zhu
14. Ke Fang
15. Haitong Fang
16. Yi Yang
17. Tao P Zhong
18. Zhongzhou Yang
19. Ke Wei
20. Wei Xie
21. Naihe Jing
22. Zhuojuan Luo
23. Chengqi Lin

• Curated by eLife

  eLife Assessment

  This study provides new single-cell multi-omics datasets that may be useful in the study of early cardiac lineages. However, the authors' conclusions regarding the mutual regulation of key regulators for cardiac specification and new cardiac lineage trajectories are inadequately supported by persuasive analysis and do not align with prior published studies. If revised to address the serious caveats adequately, the findings may be of interest to researchers in the field of cardiac development and congenital heart disease.

Reviewed by eLife

A platform for lab management, note-keeping and automation

1. Aubin Fleiss
2. Alexander S. Mishin
3. Karen S. Sarkisyan

Reviewed by Arcadia Science