Molecular Biology

Bora Bridges Aurora-A Activation and Substrate Recognition of PLK1

1. Jennifer A Miles
2. Matthew Batchelor
3. Martin Walko
4. Vanda Gunning
5. Andrew J Wilson
6. Megan H Wright
7. Richard Bayliss

Reviewed by Review Commons

Cohesin reconstitution and homologous recombination repair of DNA double-strand breaks in late mitosis

1. Jessel Ayra Plasencia
2. Sara Medina-Suárez
3. Esperanza Hernández-Carralero
4. Jonay García-Luis
5. Lorraine S Symington
6. Félix Machín

• Curated by eLife

  eLife Assessment

  This study provides convincing evidence that homologous recombination can occur in telophase-arrested cells, independently of cohesin subunits Smc 1-3. These findings are valuable as they point to investigate the role of cohesins re-association with chromatin in the allelic inter-sister repair by homologous recombination.

Reviewed by eLife

TRF2 Non-Telomeric Function is Indispensable for Neural stemness

1. Soujanya Vinayagamurthy
2. Amit Kumar Bhatt
3. Sulochana Bagri
4. Supratim Ghosh
5. Arpan Parichha
6. Arindam Maitra
7. Shantanu Chowdhury

Reviewed by Review Commons

Epigenetic delineation of the earliest cardiac lineage segregation by single-cell multi-omics

1. Peng Xie
2. Xu Jiang
3. Jingjing He
4. Qingyun Pan
5. Xianfa Yang
6. Yanying Zheng
7. Zhuanzhuan Che
8. Wenli Fan
9. Chen Wu
10. Weiheng Zheng
11. Shuhan Si
12. Kun Gao
13. Shiqi Zhu
14. Ke Fang
15. Haitong Fang
16. Yi Yang
17. Tao P Zhong
18. Zhongzhou Yang
19. Ke Wei
20. Wei Xie
21. Naihe Jing
22. Zhuojuan Luo
23. Chengqi Lin

• Curated by eLife

  eLife Assessment

  This study provides new single-cell multi-omics datasets that may be useful in the study of early cardiac lineages. However, the authors' conclusions regarding the mutual regulation of key regulators for cardiac specification and new cardiac lineage trajectories are inadequately supported by persuasive analysis and do not align with prior published studies. If revised to address the serious caveats adequately, the findings may be of interest to researchers in the field of cardiac development and congenital heart disease.

Reviewed by eLife

A platform for lab management, note-keeping and automation

1. Aubin Fleiss
2. Alexander S. Mishin
3. Karen S. Sarkisyan

Reviewed by Arcadia Science

Ribosomal RNA synthesis by RNA polymerase I is subject to premature termination of transcription

1. Chaïma Azouzi
2. Katrin Schwank
3. Sophie Queille
4. Marta Kwapisz
5. Marion Aguirrebengoa
6. Anthony Henras
7. Simon Lebaron
8. Herbert Tschochner
9. Annick Lesne
10. Frédéric Beckouët
11. Olivier Gadal
12. Christophe Dez

• Curated by eLife

  eLife Assessment

  This manuscript characterizes a mutated clone of RNA polymerase I in yeast, referred to as SuperPol, to understand the mechanisms of RNA polymerase I elongation and termination. The authors present convincing evidence that demonstrates the existence of premature termination in Pol I transcription. Overall, the characterization of this RNA pol I offers important insights into the regulation of ribosomal RNA transcription and its potential application in cancer pharmacology.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife, Review Commons

Single-cell profiling of trabecular meshwork identifies mitochondrial dysfunction in a glaucoma model that is protected by vitamin B3 treatment

1. Nicholas Tolman
2. Taibo Li
3. Revathi Balasubramanian
4. Guorong Li
5. Rebecca Pfeiffer
6. Violet Bupp-Chickering
7. Ruth A Kelly
8. Marina Simón
9. John Peregrin
10. Christa Montgomery
11. Bryan Jones
12. W Daniel Stamer
13. Jiang Qian
14. Simon WM John

• Curated by eLife

  eLife Assessment

  This study provides a fundamental advancement in our understanding of trabecular meshwork cell diversity and its role in eye pressure regulation and glaucoma using multimodal single-cell analysis, spatial validation, and functional testing that go beyond the current state-of-the-art. The study demonstrates that mitochondrial dysfunction, specifically in one of three distinct cell subtypes (TM3), contributes to elevated IOP in a genetic mouse model of glaucoma carrying a mutation in the transcription factor Lmx1b. While the identification of TM3 cells as metabolically specialized is compelling, there is somewhat limited evidence linking mitochondrial dysfunction to the Lmx1b mutation in TM3 cells.

Reviewed by eLife

Dietary sulfur amino acid restriction elicits a cold-like transcriptional response in inguinal but not epididymal white adipose tissue of male mice

1. Philip MM Ruppert
2. Aylin S Güller
3. Marcus Rosendal
4. Natasa Stanic
5. Jan-Wilhelm Kornfeld

• Curated by eLife

  eLife Assessment

  Ruppert et al. investigated how activation of thermogenesis by cold exposure (CE) and methionine restriction (MetR) impacts health and leads to weight loss in mice. The authors provided valuable datasets showing that the responses to MR and CE are tissue-specific, while MR and CE affect beige adipose similarly. Although the study is descriptive, the data analyses are solid, with well-supported conclusions drawn from the findings.

Reviewed by eLife

Loss of nephronophthisis-associated nephrocystin-1 impairs DNA damage repair in kidney organoids

1. E. Sendino Garví
2. S. Biermans
3. N.V.A.M. Knoers
4. A.M. van Eerde
5. R. Masereeuw
6. G.G. Slaats
7. A.M. van Genderen
8. M.J. Janssen

Reviewed by PREreview

Insight into the bioactivity and action mode of betulin, a candidate aphicide from plant metabolite, against aphids

1. Junxiu Wang
2. Matthana Klakong
3. Qiuyu Zhu
4. Jinting Pan
5. Yudie Duan
6. Lirong Wang
7. Yong Li
8. Jiangbo Dang
9. Danlong Jing
10. Hong Zhou

• Curated by eLife

  eLife Assessment

  This important study identifies a plant-derived metabolite, betulin, as an effective natural insecticide against aphids and uncovers its specific molecular target. The evidence is compelling, combining greenhouse and field efficacy trials with rigorous molecular, genetic, and electrophysiological approaches that converge on a conserved binding site in the aphid GABA receptor. While additional work is needed to fully assess potential off-target effects and ecological safety, the study provides a strong mechanistic foundation. These findings will be of interest to researchers in plant biology, chemical ecology, and sustainable pest management.

Reviewed by eLife