Molecular Biology

Nucleolar dynamics are determined by the ordered assembly of the ribosome

1. Jessica Sheu-Gruttadauria
2. Xiaowei Yan
3. Nico Stuurman
4. Riley O. Ogrean
5. Sabrina Lin
6. Stephen N. Floor
7. Ronald D. Vale

Reviewed by Review Commons

In extracto cryo-EM reveals eEF2 as a major hibernation factor on 60S and 80S particles

1. Zahra Seraj
2. Ximena Zottig
3. Chun-Ying Huang
4. Anna B Loveland
5. Stephen Diggs
6. Emily Sholi
7. Nikolaus Grigorieff
8. Andrei A Korostelev

• Curated by eLife

  eLife Assessment

  In this important work, it is demonstrated that certain high-resolution cryo-EM structures can be obtained by using concentrated cell extracts without purification. The compelling results with the mammalian ribosomes demonstrate the utility of this approach for this molecule and complexes with elongation factor 2. Moreover, this work also demonstrates the utility of 2D template matching for particle picking for structure determination by single-particle averaging pipelines.

Reviewed by eLife

Highly frequent undesired insertional mutagenesis during Drosophila genome editing

1. Emma Källstig
2. Evelyne Ruchti
3. Medha Raman
4. Jamshid Asadzadeh
5. Bernard L. Schneider
6. Brian D. McCabe

Reviewed by Review Commons

Patterns of aDNA damage through time and environments—lessons from herbarium specimens

1. Stefano Porrelli
2. Alice Fornasiero
3. Hong Phuong Le
4. Wenzhe Yin
5. María E Navarrete Rodríguez
6. Nahed Mohammed
7. Axel Himmelbach
8. Andrew C Clarke
9. Nils Stein
10. Paul J Kersey
11. Rod A Wing
12. Rafal M Gutaker

Reviewed by GigaScience

CUTS RNA Biosensor for the Real-Time Detection of TDP-43 Loss-of-Function

1. Longxin Xie
2. Jessica Merjane
3. Cristian A Bergmann
4. Jiazhen Xu
5. Shruthi Balasubramaniyan
6. Bryan Hurtle
7. Charleen T Chu
8. Christopher J Donnelly

• Curated by eLife

  eLife Assessment

  This important study developed a new sensor for TDP-43 activity that is sensitive and robust that should strongly impact the field's ability to monitor whether TDP-43 is functional or not. The evidence, though limited to cell culture, is compelling and is the first demonstration that a GFP on/off system can be used to assess genetic TDP-43 mutants as well as loss of soluble TDP-43.

Reviewed by eLife

Liquid-Liquid Phase Separation-mediated formation of amyloid fibrils from DcpS scavenger enzymes

1. Aleksandra Ferenc-Mrozek
2. Maria Winiewska-Szajewska
3. Hanna Nieznańska
4. Wojciech Dzwolak
5. Maciej Łukaszewicz

Reviewed by preLights