Molecular Biology

Development of Loop Mediated Isothermal Amplification for Rapid and Sensitive Detection of Enteroaggregative Escherichia coli (EAEC)

1. Alazar Amare Amdiyee
2. Tesfaye Sisay Tessema

Reviewed by PREreview

Global molecular landscape of early MASLD progression in obesity

1. Qing Zhao
2. William De Nardo
3. Ruoyu Wang
4. Yi Zhong
5. Umur Keles
6. Gabrielé Sakalauskaite
7. Li Na Zhao
8. Huiyi Tay
9. Sonia Youhanna
10. Mengchao Yan
11. Ye Xie
12. Youngrae Kim
13. Sungdong Lee
14. Rachel Liyu Lim
15. Guoshou Teo
16. Pradeep Narayanaswamy
17. Paul R Burton
18. Volker M Lauschke
19. Hyungwon Choi
20. Matthew J Watt
21. Philipp Kaldis

• Curated by eLife

  eLife Assessment

  The authors provide a useful integrated analytical approach to investigating MASLD focused on diverse multiomic integration methods. The strength of evidence for this new resource is solid, as analyses highlight the importance of previously-described pathophysiologic processes, as well as unveil several new mechanisms as key features of MASLD in obese patients.

Reviewed by eLife, Review Commons

Orphan nuclear receptors recruit TRIM28 to promote telomeric H3K9me3 for the alternative lengthening of telomeres pathway

1. Chia-Tsen Tsai
2. Venus Marie Gaela
3. Hsuan-Yu Hsia
4. Yu-Chen Huang
5. Liuh-Yow Chen

Reviewed by Review Commons

Competition for the conserved branch point sequence influences physiological outcomes in pre-mRNA splicing

1. Karen Larissa Pereira de Castro
2. Jose M Abril
3. Kuo-Chieh Liao
4. Haiping Hao
5. John Paul Donohue
6. William K Russell
7. W Samuel Fagg

• Curated by eLife

  eLife Assessment

  This important manuscript provides insights into the competition between Splicing Factor 1 (SF1) and Quaking (QKI) for binding at the ACUAA branch point sequence in a model intron, regulating exon inclusion. The study employs convincing, rigorous transcriptomic, proteomic, and reporter assays, with both mammalian cell culture and yeast models.

Reviewed by eLife

Role of ADMA-histones in dual-strand piRNA source loci recognition by Rhino

1. Raku Saito
2. Hirotsugu Ishizu
3. Ritsuko Harigai
4. Kensaku Murano
5. Yurika Namba
6. Mikiko C Siomi

• Curated by eLife

  eLife Assessment

  This valuable study identifies asymmetric dimethylarginine (ADMA) modification of histones as a potential key determinant of the initial genomic binding of Rhino, a Drosophila-specific chromatin protein essential for piRNA cluster specification. The authors provide correlative genomic and imaging data to support their model, although functional validation of the proposed mechanism remains incomplete. Testing the redundancy between dART4 and dART1, which together could affect the prominent piRNA loci, in addition to the minor ones investigated in the manuscript, may change our assessment.

Reviewed by eLife

Opposing CTCF and GATA4 activities set the pace of chromatin topology remodeling during cardiomyogenesis

1. Silvia Becca
2. Sara Bianchi
3. Elisa M. Hahn
4. Kirsten E. Snijders
5. Lukasz Truszkowski
6. Anna Krepelova
7. Francesco Neri
8. Davide Cacchiarelli
9. Sasha Mendjan
10. Elisa Balmas
11. Alessandro Bertero

Reviewed by Review Commons

Lipid-Mediated Control of ER Function During Apoptosis

1. José Luis Garrido-Huarte
2. Josep Fita-Torró
3. Amparo Pascual-Ahuir
4. Markus Proft

Reviewed by Arcadia Science

Dietary sulfur amino acid restriction elicits a cold-like transcriptional response in inguinal but not epididymal white adipose tissue of male mice

1. Philip MM Ruppert
2. Aylin S Güller
3. Marcus Rosendal
4. Natasa Stanic
5. Jan-Wilhelm Kornfeld

• Curated by eLife

  eLife Assessment

  The present study employed transcriptomics to investigate the impact of methionine restriction (MR) and cold exposure (CE) on liver and adipose tissues in mice. The authors demonstrate that responses to MR and CE are tissue-specific, while both MR and CE have a similar effect on beige adipose tissue. While these findings are somewhat descriptive, this work is considered important, as it provides a comprehensive resource for enhancing our understanding of these lifestyle interventions. The study is of high scientific quality, and the analyses are convincing.

Reviewed by eLife

Proximity labeling reveals ZFP36L1 as a central hub for post-transcriptional regulation networks in T cells

1. Anouk P. Jurgens
2. Branka Popović
3. Floris P.J. van Alphen
4. Leyma Wardak
5. Antonia Bradarić
6. Sander Engels
7. Carmen van der Zwaan
8. Maartje van den Biggelaar
9. Arie J. Hoogendijk
10. Julien Béthune
11. Monika C. Wolkers

Reviewed by Review Commons

NASP functions in the cytoplasm to prevent histone H3 aggregation during early embryogenesis

1. Mohit Das
2. Eli Coronado-Chavez
3. Anusha D Bhatt
4. Reyhaneh Tirgar
5. Amanda A Amodeo
6. Jared Nordman

Reviewed by Review Commons