Molecular Biology

Interplay between cohesin and TORC1 links chromosome segregation and gene expression to environmental changes

1. Dorian Besson
2. Sabine Vaur
3. Stéphanie Vazquez
4. Sylvie Tournier
5. Yannick Gachet
6. Adrien Birot
7. Stéphane Claverol
8. Adèle Marston
9. Anastasios Damdimopoulos
10. Karl Ekwall
11. Jean-Paul Javerzat

• Curated by eLife

  eLife Assessment

  This important study describes a new link between nutrient signaling and chromosome regulation, providing compelling evidence that reduced activity in the central nutrient-sensing pathway governed by TORC1 improves chromosome stability and alters gene expression in S. pombe through effects on cohesin. While the biological importance of this newly described circuit is not yet fully known, and some data would benefit from further clarification, the overall body of evidence supports the main conclusions.

Reviewed by eLife

Single cell transcriptomics and development of gametocyte-specific molecular markers for avian malaria parasites

1. A Berthomieu
2. S Gandon
3. AM Talman
4. A Rivero

Reviewed by PREreview

Mechanistic insights into transcriptional regulation of ARHGAP36 expression identify a factor predictive of neuroblastoma survival

1. Serhiy Havrylov
2. Armin M Gamper
3. Ordan J Lehmann

• Curated by eLife

  eLife Assessment

  This important study examines the potential role of ARHGAP36 transcriptional regulation by FOXC1 in controlling sonic hedgehog signaling in human neuroblastoma. While there are many solid findings that strongly support this signaling pathway, there are some aspects of the study that are underdeveloped.

Reviewed by eLife

Insights from aquaporin structures into drug-resistant sleeping sickness

1. Modestas Matusevicius
2. Robin A Corey
3. Marcos Gragera
4. Keitaro Yamashita
5. Teresa Sprenger
6. Marzuq A Ungogo
7. James N Blaza
8. Pablo Castro-Hartmann
9. Dimitri Y Chirgadze
10. Sundeep Chaitanya Vedithi
11. Pavel Afanasyev
12. Roberto Melero
13. Rangana Warshamanage
14. Anastasiia Gusach
15. Jose Maria Carazo
16. Mark Carrington
17. Tom L Blundell
18. Garib Murshudov
19. Phillip Stansfeld
20. Mark Sansom
21. Harry P de Koning
22. Christopher G Tate
23. Simone N Weyand

• Curated by eLife

  eLife Assessment

  In this important study, the authors set out to determine the molecular interactions between the AQP2 from Trypanosoma brucei (TbAQP2) and the trypanocidal drugs pentamidine and melarsoprol to understand how TbAQP2 mutations lead to drug resistance. Using cryo-EM, molecular dynamics simulations, and lysis assays the authors present convincing evidence that mutations in TbAQP2 make permeation of trypanocidal drugs energetically less favourable, and that this impacts the ability of drugs to achieve a therapeutic dose. Overall, this data will be of interest for those working on aquaporins, and development of trypanosomiasis drugs as well as drugs targeting aquaporins in general.

Reviewed by eLife

Cryo-EM Reveals Regulatory Mechanisms Governing Substrate Selection and Activation of Human LONP1

1. Jeffrey T Mindrebo
2. Lauren Alexandrescu
3. Jennifer R Baker
4. Garret Wang
5. Gabriel C Lander

• Curated by eLife

  eLife Assessment

  This valuable study presents EM structures of new conformational states of the LONP1 AAA+ protease in conjunction with the mitochondrial protein substrates (StAR, TFAM), along with biochemical functional assays. The EM structures revealed new conformational states in a closed configuration. The structures and associated functional results are solid. However, a notable weakness is the absence of substrates found threaded through the ATPase pores.

Reviewed by eLife

On the path to reference genomes for all biodiversity: lessons learned and laboratory protocols created in the Sanger Tree of Life core laboratory over the first 2000 species

1. Caroline Howard
2. Amy Denton
3. Benjamin Jackson
4. Adam Bates
5. Jessie Jay
6. Halyna Yatsenko
7. Priyanka Sethu Raman
8. Abitha Thomas
9. Graeme Oatley
10. Raquel Vionette do Amaral
11. Zeynep Ene Göktan
12. Juan Pablo Narváez Gómez
13. Isabelle Clayton Lucey
14. Elizabeth Sinclair
15. Michael A. Quail
16. Mark Blaxter
17. Kerstin Howe
18. Mara K. N. Lawniczak

Reviewed by GigaScience

CryoDataBot: a pipeline to curate cryoEM datasets for AI-driven structural biology

1. Qibo Xu
2. Leon Wu
3. Michael Rebelo
4. Shi Feng
5. Xinye Yu
6. Farhanaz Farheen
7. Daisuke Kihara
8. Z. Hong Zhou

Reviewed by GigaScience

Herbivorous insects independently evolved salivary effectors to regulate plant immunity by destabilizing the malectin-LRR RLP NtRLP4

1. Xin Wang
2. Jia-Bao Lu
3. Yi-Zhe Wang
4. Xu-Hong Zhou
5. Jian-Ping Chen
6. Chuan-Xi Zhang
7. Jun-Min Li
8. Hai-Jian Huang

• Curated by eLife

  eLife Assessment

  This study provides an important contribution by showing that whiteflies and planthoppers use salivary effectors to suppress plant immunity through the receptor-like protein RLP4, suggesting convergent evolution in these insect lineages. The topic is of clear interest for understanding plant-insect interactions and offers ideas that could stimulate further research in the field. However, the strength of evidence is incomplete, as some aspects of the data and experimental design limit the extent to which the main claims are fully supported.

Reviewed by eLife

Endogenous Real Time Imaging Reveals Dynamic Chromosomal Mobility During Ligand-Mediated Transcriptional Burst Events

1. Susan Wang
2. Thomas Suter
3. Amir Gamliel
4. Yeeun Kim
5. Sreejith J Nair
6. Soohwan Oh
7. Feng Yang
8. Kenneth A Ohgi
9. Tobias Wagner
10. Steven Gan
11. Michael G Rosenfeld

• Curated by eLife

  eLife Assessment

  This manuscript presents significant and important work that advances single-molecule imaging technology of transcription with simultaneous analysis of several parameters. However, currently, the evidence is incomplete and requires further quantitation/description of the technologies used, further controls, and additional analysis of the data by other methods.

Reviewed by eLife

Fibro-Adipogenic Progenitors require autocrine IGF-I in homeostatic and regenerating skeletal muscle

1. Yangyi E. Luo
2. Zoe Abe-Teh
3. Tarek Y. Alsaghir
4. Li-Ying Kuo
5. Fahong Yu
6. Bradley E. Stoker
7. Ambili Bai Appu
8. Yumei Zhou
9. Feng Yue
10. Daniel Kopinke
11. Elisabeth R. Barton

Reviewed by preLights