Molecular Biology

Highly frequent undesired insertional mutagenesis during Drosophila genome editing

1. Emma Källstig
2. Evelyne Ruchti
3. Medha Raman
4. Jamshid Asadzadeh
5. Bernard L. Schneider
6. Brian D. McCabe

Reviewed by Review Commons

Patterns of aDNA Damage Through Time and Environments – lessons from herbarium specimens

1. Stefano Porrelli
2. Alice Fornasiero
3. Hong Phuong Le
4. Wenzhe Yin
5. Maria Navarrete Rodriguez
6. Nahed Mohammed
7. Axel Himmelbach
8. Andrew C. Clarke
9. Nils Stein
10. Paul J. Kersey
11. Rod A. Wing
12. Rafal M. Gutaker

Reviewed by GigaScience

CUTS RNA Biosensor for the Real-Time Detection of TDP-43 Loss-of-Function

1. Longxin Xie
2. Jessica Merjane
3. Cristian A Bergmann
4. Jiazhen Xu
5. Shruthi Balasubramaniyan
6. Bryan Hurtle
7. Charleen T Chu
8. Christopher J Donnelly

• Curated by eLife

  eLife Assessment

  This important study developed a new sensor for TDP-43 activity that is sensitive and robust that should strongly impact the field's ability to monitor whether TDP-43 is functional or not. The evidence, though limited to cell culture, is compelling and is the first demonstration that a GFP on/off system can be used to assess genetic TDP-43 mutants as well as loss of soluble TDP-43.

Reviewed by eLife

Liquid-Liquid Phase Separation-mediated formation of amyloid fibrils from DcpS scavenger enzymes

1. Aleksandra Ferenc-Mrozek
2. Maria Winiewska-Szajewska
3. Hanna Nieznańska
4. Wojciech Dzwolak
5. Maciej Łukaszewicz

Reviewed by preLights

Oxidative stress triggers RNAPII arrest through PARylation and DNA damage

1. Quentin A. Thomas
2. Liyang Wu
3. Emma Lesage
4. Henriette K. M. Iversen
5. David López Martínez
6. Smaragda Kompocholi
7. Haiyue Liu
8. Nicolás Nieto Moreno
9. Lea H. Gregersen

Reviewed by preLights

Frataxin depletion leads to decreased soma size and activation of AMPK metabolic pathway in dorsal root ganglia sensory neurons

1. Olivier Griso
2. Deepika Chellapandi
3. Amélie Weiss
4. Ioannis Manolaras
5. Hélène Puccio

Reviewed by Review Commons

A lipoprotein partner for the Escherichia coli outer membrane protein TolC

1. Jim Horne
2. Elise Kaplan
3. Ben Jin
4. Kieran Abbott
5. Victor Flores
6. Emmanouela Petsolari
7. Jan Gradon
8. Yvette Ntsogo
9. Andrzej Harris
10. Dingquan Yu
11. Ashraf Zarkan
12. Ben F Luisi

• Curated by eLife

  eLife Assessment

  In this fundamental work Horne et al present compelling evidence that YbjP is a novel binding partner of the TolC channel protein. The YbjP is characterized using cryo-EM, and its role probed using pull-down experiments, in vivo crosslinking, functional assays along with phylogenetic analysis which are all properly performed and presented and support the main conclusions. While the study does not identify a clear role for this protein, the revised manuscript offers improved clarity and contributes invaluable insight into membrane transport and antimicrobial resistance.

Reviewed by eLife

Interplay between cohesin and TORC1 links chromosome segregation and gene expression to environmental changes

1. Dorian Besson
2. Sabine Vaur
3. Stéphanie Vazquez
4. Sylvie Tournier
5. Yannick Gachet
6. Adrien Birot
7. Stéphane Claverol
8. Adèle Marston
9. Anastasios Damdimopoulos
10. Karl Ekwall
11. Jean-Paul Javerzat

• Curated by eLife

  eLife Assessment

  This important study presents a compelling link between nutrient signaling and chromosome regulation, demonstrating that reduced activity in a central nutrient-sensing pathway improves chromosome stability and alters gene expression through effects on cohesin. The convincing evidence from a combination of genetic, biochemical and cell biological approaches supports a model in which TORC1-dependent phosphorylation of Mis4 and the cohesin subunit Psm1/Smc1 can modulate cohesin loading to enhance faithful chromosome transmission. While the underlying mechanisms and biological importance of this newly described circuit are not yet fully known, the overall body of evidence is strong and supports the main conclusions.

Reviewed by eLife

Herbivorous insects independently evolved salivary effectors to regulate plant immunity by destabilizing the malectin-LRR RLP NtRLP4

1. Xin Wang
2. Jia-Bao Lu
3. Yi-Zhe Wang
4. Xu-Hong Zhou
5. Jian-Ping Chen
6. Chuan-Xi Zhang
7. Jun-Min Li
8. Hai-Jian Huang

• Curated by eLife

  eLife Assessment

  This study provides an important contribution by showing that whiteflies and planthoppers use salivary effectors to suppress plant immunity through the receptor-like protein RLP4, suggesting convergent evolution in these insect lineages. The topic is of clear interest for understanding plant-insect interactions and offers ideas that could stimulate further research in the field. The authors provide convincing evidence for the functional roles of the salivary effectors.

Reviewed by eLife

Protein secretion routes in fungi are mostly determined by the length of the hydrophobic helix in the signal peptide

1. Tristan Sones-Dykes
2. Edward Wallace

Reviewed by Review Commons