Molecular Biology

The ASC inflammasome adapter controls the extent of peripheral protein aggregate deposition in inflammation-associated amyloidosis

1. Marco Losa
2. Marc Emmenegger
3. Pierre De Rossi
4. Patrick M Schürch
5. Tetiana Serdiuk
6. Niccolò Pengo
7. Danaëlle Capron
8. Dimitri Bieli
9. Niels J Rupp
10. Manfredi C Carta
11. Karl J Frontzek
12. Veronika Lysenko
13. Regina R Reimann
14. Asvin KK Lakkaraju
15. Mario Nuvolone
16. Gunilla T Westermark
17. K. Peter R. Nilsson
18. Magdalini Polymenidou
19. Alexandre P. A. Theocharides
20. Simone Hornemann
21. Paola Picotti
22. Adriano Aguzzi

Reviewed by Review Commons

Modeling the consequences of age-linked rDNA hypermethylation with dCas9-directed DNA methylation in human cells

1. Yana Blokhina
2. Abigail Buchwalter

Reviewed by Review Commons

Aberrant methylation and expression of TNXB promote chondrocyte apoptosis and extracullar matrix degradation in hemophilic arthropathy via AKT signaling

1. Jiali Chen
2. Qinghe Zeng
3. Xu Wang
4. Rui Xu
5. Weidong Wang
6. Yuliang Huang
7. Qi Sun
8. Wenhua Yuan
9. Pinger Wang
10. Di Chen
11. Peijian Tong
12. Hongting Jin

• Curated by eLife

  eLife assessment

  This important study identifies the TNXB-AKT pathway as a potential mechanism underlying hemophilia-associated cartilage degeneration. The evidence supporting the conclusions is convincing, with murine and human patient evidence as well as genome-wide DNA methylation analysis. This paper would be of interest to cell biologists and biochemists working on the field of musculoskeletal disorders.

Reviewed by eLife

Stomatin-like protein 2 senses oxidative stress through the interaction with phosphatidic acid to promote mitochondrial unfolded protein response

1. Maria Paulina Castelo Rueda
2. Irene Pichler
3. Karolina Musilova
4. Stanislav Kmoch
5. Peter P. Pramstaller
6. Ales Hnizda
7. Andrew A. Hicks
8. Roman Vozdek

Reviewed by Arcadia Science

Lipid-Based Transfection of Zebrafish Embryos: A Robust Protocol for Nucleic Acid Delivery in Zebrafish

1. Aslihan Terzi
2. Tiger Lao
3. Adrian Jacobo

Reviewed by preLights

Late-life dietary folate restriction reduces biosynthetic processes without compromising healthspan in mice

1. Heidi M. Blank
2. Staci E. Hammer
3. Laurel Boatright
4. Courtney Roberts
5. Katarina E. Heyden
6. Aravindh Nagarajan
7. Mitsuhiro Tsuchiya
8. Marcel Brun
9. Charles D. Johnson
10. Patrick J. Stover
11. Raquel Sitcheran
12. Brian K. Kennedy
13. L. Garry Adams
14. Matt Kaeberlein
15. Martha S. Field
16. David W. Threadgill
17. Helene L. Andrews-Polymenis
18. Michael Polymenis

Reviewed by Review Commons

Comparative transcriptomics reveal a novel tardigrade-specific DNA-binding protein induced in response to ionizing radiation

1. Marwan Anoud
2. Emmanuelle Delagoutte
3. Quentin Helleu
4. Alice Brion
5. Evelyne Duvernois-Berthet
6. Marie As
7. Xavier Marques
8. Khadija Lamribet
9. Catherine Senamaud-Beaufort
10. Laurent Jourdren
11. Annie Adrait
12. Sophie Heinrich
13. Geraldine Toutirais
14. Sahima Hamlaoui
15. Giacomo Gropplero
16. Ilaria Giovannini
17. Loic Ponger
18. Marc Geze
19. Corinne Blugeon
20. Yohann Couté
21. Roberto Guidetti
22. Lorena Rebecchi
23. Carine Giovannangeli
24. Anne De Cian
25. Jean-Paul Concordet

• Curated by eLife

  eLife assessment

  This study offers valuable insight into the remarkable resistance of tardigrades to ionizing radiation by showing that radiation treatment induces a suite of DNA repair proteins and by identifying a strongly induced tardigrade-specific DNA-binding protein that can reduce the number of double-strand breaks in human U2OS cells. The evidence of upregulation of repair proteins is convincing, and the case for a role of the newly identified protein in repair can be strengthened as genetic tools for tardigrades become better developed. The results will interest the fields of DNA repair and radiobiology as well as tardigrade biologists.

Reviewed by eLife

Txnip deletions and missense alleles prolong the survival of cones in a retinitis pigmentosa mouse model

1. Yunlu Xue
2. Yimin Zhou
3. Constance L Cepko

• Curated by eLife

  eLife assessment

  This fundamental study advances our understanding of the cell specific treatment of cone photoreceptor degeneration by Txnip. The evidence supporting the conclusions is compelling with rigorous genetic manipulation of Txnip mutations. The work will be of broad interest to vision researchers, cell biologists and biochemists.

Reviewed by eLife

Inhibition of miR-199b-5p reduces pathological alterations in osteoarthritis by potentially targeting Fzd6 and Gcnt2

1. Tong Feng
2. Qi Zhang
3. Si-Hui Li
4. Yan-ling Ping
5. Mu-qiu Tian
6. Shuan-hu Zhou
7. Xin Wang
8. Jun-Meng Wang
9. Fan-Rang Liang
10. Shu-Guang Yu
11. Qiao-Feng Wu

• Curated by eLife

  eLife assessment

  This valuable study reports that miR-199b-5p is elevated in human osteoarthritis patients. There is solid evidence for the finding that inhibiting miR-199b-5p alleviates symptoms in mice with knee osteoarthritis. Additionally, potential targets of miR-199b-5p are identified but whether miR-199b-5p truly functions through Fzd6 and/or Gcnt2 requires further investigation.

Reviewed by eLife

Whole blood transcriptional profiles and the pathogenesis of tuberculous meningitis

1. Hoang Thanh Hai
2. Le Thanh Hoang Nhat
3. Trinh Thi Bich Tram
4. Do Dinh Vinh
5. Artika P Nath
6. Joseph Donovan
7. Nguyen Thi Anh Thu
8. Dang Van Thanh
9. Nguyen Duc Bang
10. Dang Thi Minh Ha
11. Nguyen Hoan Phu
12. Ho Dang Trung Nghia
13. Le Hong Van
14. Michael Inouye
15. Guy E Thwaites
16. Nguyen Thuy Thuong Thuong

• Curated by eLife

  eLife assessment

  In this valuable study, the authors investigate the transcriptional landscape of tuberculous meningitis. They reveal potentially significant molecular differences contributed by HIV co-infection, and derive a prognostic model to predict mortality combining a gene expression signature with clinical parameters. Whilst some of the evidence presented is compelling, the bioinformatics analysis remains limited and cannot be used to make causal inferences and conclusions about immunopathogenesis for tuberculous meningitis. The work will be of broad interest to the infectious disease community however, further validation of the findings is critical for future utility.

Reviewed by eLife