Molecular Biology

CDK-mediated phosphorylation of PNKP is required for end-processing of single-strand DNA gaps on Okazaki fragments and genome stability

1. Kaima Tsukada
2. Rikiya Imamura
3. Tomoko Miyake
4. Kotaro Saikawa
5. Mizuki Saito
6. Naoya Kase
7. Lingyan Fu
8. Masamichi Ishiai
9. Yoshihisa Matsumoto
10. Mikio Shimada

Reviewed by Review Commons

OGT prevents DNA demethylation and suppresses the expression of transposable elements in heterochromatin by restraining TET activity genome-wide

1. Hugo Sepulveda
2. Xiang Li
3. Leo J. Arteaga-Vazquez
4. Isaac F. López-Moyado
5. Melina Brunelli
6. Lot Hernández-Espinosa
7. Xiaojing Yue
8. J. Carlos Angel
9. Caitlin Brown
10. Zhen Dong
11. Natasha Jansz
12. Fabio Puddu
13. Aurélie Modat
14. Jamie Scotcher
15. Páidí Creed
16. Patrick H. Kennedy
17. Cindy Manriquez-Rodriguez
18. Samuel A. Myers
19. Robert Crawford
20. Geoffrey J. Faulkner
21. Anjana Rao

Reviewed by preLights

Regulated mRNA recruitment in dinoflagellates is reflected in hyper-variable mRNA spliced leaders and novel eIF4Es

1. Grant D. Jones
2. Ernest P. Williams
3. Saddef Haq
4. Tsvetan R. Bachvaroff
5. M. Basanta Sanchez
6. Allen R. Place
7. Rosemary Jagus

• Curated by eLife

  eLife assessment

  This important study provides previously unappreciated insights into the functions of protist eIF4E 5'mRNA cap-binding protein family members, thereby contributing to a better understanding of translation regulation in these organisms. The authors provide solid evidence to support the major conclusions of the article. However, the study may further benefit from establishing whether all of the eIF4E family members are indeed involved in translation and more direct evidence for the selectivity of their binding.

Reviewed by eLife

Clusters of lineage-specific genes are anchored by ZNF274 in repressive perinucleolar compartments

1. Martina Begnis
2. Julien Duc
3. Sandra Offner
4. Delphine Grun
5. Shaoline Sheppard
6. Olga Rosspopoff
7. Didier Trono

Reviewed by preLights

The substrate-binding domains of the osmoregulatory ABC importer OpuA transiently interact

1. Marco van den Noort
2. Panagiotis Drougkas
3. Cristina Paulino
4. Bert Poolman

• Curated by eLife

  eLife assessment

  The OpuA Type I ABC importer uses two substrate binding domains to capture extracellular glycine betaine and present the substrate to the transmembrane domain for subsequent transport and correction of internal dehydration. This study presents valuable findings addressing the question of whether the two substrate binding domains of OpuA dock and physically interact in a salt-dependent manner. The single-molecule fluorescence resonance energy transfer and cryogenic electron microscopy data that are presented provide convincing support for the existence of a transient interaction between the substrate binding domains that depends on ionic strength, laying a foundation for future studies exploring how this interaction is involved in the overall transport mechanism.

Reviewed by eLife

Circadian oscillation of perireceptor events influence olfactory sensitivity in diurnal and nocturnal mosquitoes

1. Tanwee Das De
2. Julien Pelletier
3. Satyajeet Gupta
4. Madhavinadha Prasad Kona
5. Om P. Singh
6. Rajnikant Dixit
7. Rickard Ignell
8. Krishanpal Karmodiya

Reviewed by Review Commons

Mapping the molecular motions of 5-HT3 serotonin-gated channel by voltage-clamp fluorometry

1. Laurie Peverini
2. Sophie Shi
3. Karima Medjebeur
4. Pierre-Jean Corringer

• Curated by eLife

  eLife assessment

  This valuable study applies voltage clamp fluorometry to provide new information about the function of serotonin-gated ion channels 5-HT3AR. The authors convincingly investigate structural changes inside and outside the orthosteric site elicited by agonists, partial agonists, and antagonists, helping to annotate existing cryo-EM structures. This work confirms that the activation of 5-HT3 receptors is similar to other members of this well-studied receptor superfamily. The work will be of interest to scientists working on channel biophysics but also drug development targeting ligand-gated ion channels.

Reviewed by eLife

Preeclampsia in mice carrying fetuses with APOL1 risk variants

1. Teruhiko Yoshida
2. Khun Zaw Latt
3. Shashi Shrivastav
4. Huiyan Lu
5. Kimberly J. Reidy
6. Jennifer R. Charlton
7. Yongmei Zhao
8. Cheryl A. Winkler
9. Sandra E. Reznik
10. Avi Z. Rosenberg
11. Jeffrey B. Kopp

Reviewed by Arcadia Science

Intronic polyadenylation isoforms in the 5’ part of genes constitute a source of microproteins and are involved in cell response to cisplatin

1. Alexandre Devaux
2. Iris Tanaka
3. Mandy Cadix
4. Amélie Heneman-Masurel
5. Sophie Michallet
6. Quentin Fouilleul
7. Alina Chakraborty
8. Céline M. Labbe
9. Nicolas Fontrodona
10. Jean-Baptiste Claude
11. Marc Deloger
12. Pierre Gestraud
13. Ludovic Tessier
14. Hussein Mortada
15. Sonia Lameiras
16. Virginie Raynal
17. Sylvain Baulande
18. Nicolas Servant
19. Didier Auboeuf
20. Béatrice Eymin
21. Stéphan Vagner
22. Martin Dutertre

Reviewed by Review Commons

N-cadherin directs the collective Schwann cell migration required for nerve regeneration through Slit2/3-mediated contact inhibition of locomotion

1. Julian JA Hoving
2. Elizabeth Harford-Wright
3. Patrick Wingfield-Digby
4. Anne-Laure Cattin
5. Mariana Campana
6. Alex Power
7. Toby Morgan
8. Erica Torchiaro
9. Victor Quereda
10. Alison C Lloyd

• Curated by eLife

  eLife assessment

  This study presents an interesting set of findings that connects N-cadherin and glypican-4 to Slit signaling during the regulation of contact inhibition of locomotion of Schwann cells in culture. Solid evidence is provided showing that N-cadherin not only regulates cell recognition but also proper trafficking of Slit to the cell surface. An ex-vivo model demonstrates the importance of Slit signaling during cell migration but the molecular details of how N-cadherin traffics Slit to the surface and role of glypican are unclear. The data would have been strengthened with a similar interrogation of N-cadherin in this system. The work will be of interest to cell biologists studying the mechanisms behind peripheral nervous system regeneration.

Reviewed by eLife