Molecular Biology

TAX1BP1 recruits ATG9 vesicles through SCAMP3 binding

1. Yutaro Hama
2. Yoshitaka Kurikawa
3. Takahide Matsui
4. Noboru Mizushima
5. Hayashi Yamamoto

Reviewed by Review Commons

Multiple cullin-associated E3 ligases regulate cyclin D1 protein stability

1. Ke Lu
2. Ming Zhang
3. Guizheng Wei
4. Guozhi Xiao
5. Liping Tong
6. Di Chen

• Curated by eLife

  eLife assessment

  This paper is of interest to cell biologists studying the mechanisms of protein posttranslational modifications. The study investigates Cullin-RING ubiquitin E3 ligases (CRLs) that regulate cyclin D1 protein stability in cells by utilizing siRNA screening and ectopic expression approaches. By screening a siRNA library containing different E3 ligases, the authors identified a previously uncharacterized combination of Cullin 1-7 and associated E3 ligases (Keap1-CUL3, DDB2-CUL4A/4B, WSB2-CUL2/5, and RBX1-CUL1-7) that are important for cyclin D1 ubiquitination and proteasome-mediated degradation.

Reviewed by eLife

Tardigrades dramatically upregulate DNA repair pathway genes in response to ionizing radiation

1. Courtney M. Clark-Hachtel
2. Jonathan D. Hibshman
3. Tristan De Buysscher
4. Bob Goldstein

Reviewed by preLights

ZMYM2 controls human transposable element transcription through distinct co-regulatory complexes

1. Danielle J Owen
2. Elisa Aguilar-Martinez
3. Zongling Ji
4. Yaoyong Li
5. Andrew D Sharrocks

Reviewed by eLife

Transcription-independent hold of the G1/S transition is exploited to cope with DNA replication stress

1. Yue Jin
2. Guoqing Lan
3. Jiaxin Zhang
4. Haoyuan Sun
5. Li Xin
6. Qinhong Cao
7. Chao Tang
8. Xiaojing Yang
9. Huiqiang Lou
10. Wenya Hou

Reviewed by Review Commons

The helicase domain of human Dicer prevents RNAi-independent activation of antiviral and inflammatory pathways

1. Morgane Baldaccini
2. Léa Gaucherand
3. Béatrice Chane-Woon-Ming
4. Mélanie Messmer
5. Floriane Gucciardi
6. Sébastien Pfeffer

Reviewed by Review Commons

Multiplex DNA fluorescence in situ hybridization to analyze maternal vs. paternal C. elegans chromosomes

1. Silvia Gutnik
2. Ahilya Sawh
3. Susan E. Mango

Reviewed by Review Commons

Identification of a carbohydrate recognition motif of purinergic receptors

1. Lifen Zhao
2. Fangyu Wei
3. Xinheng He
4. Antao Dai
5. Dehua Yang
6. Hualiang Jiang
7. Liuqing Wen
8. Xi Cheng

• Curated by eLife

  eLife assessment

  Purines are native molecules that affect processes in the immune system, among others. The manuscript describes a valuable investigation of the mode of binding of purines, especially their carbohydrate moiety, to human receptors in cell culture and by computer-based modelling. Solid evidence is presented about the way purines interact with and activate two receptors.

Reviewed by eLife

Genetic validation of PfFKBP35 as an antimalarial drug target

1. Basil T Thommen
2. Jerzy M Dziekan
3. Fiona Achcar
4. Seth Tjia
5. Armin Passecker
6. Katarzyna Buczak
7. Christin Gumpp
8. Alexander Schmidt
9. Matthias Rottmann
10. Christof Grüring
11. Matthias Marti
12. Zbynek Bozdech
13. Nicolas MB Brancucci

• Curated by eLife

  eLife assessment

  FKBP35 is the only FK506-binding protein present in the malaria-causing parasite Plasmodium falciparum, and has been considered a promising drug target due to its high affinity to the macrolide compound FK506, an immunosuppressant with antiplasmodial activity. This study demonstrates the essentiality of FKBP35 in parasite growth, based on compelling genetic evidence. The data also suggest that FK506 may exert its antimalarial activity through FKBP35-independent mechanisms that have not yet been characterised. This important study will be of interest to scientists working on the parasite biology and antimalarial drug development.

Reviewed by eLife

CRISPR-dependent Base Editing Screens Identify Separation of Function Mutants of RADX with Altered RAD51 Regulatory Activity

1. Madison B. Adolph
2. Atharv S. Garje
3. Swati Balakrishnan
4. Florian Morati
5. Mauro Modesti
6. Walter J. Chazin
7. David Cortez

Reviewed by preLights