Molecular Biology

Cohesin still drives homologous recombination repair of DNA double-strand breaks in late mitosis

1. Jessel Ayra-Plasencia
2. Lorraine Symington
3. Félix Machín

• Curated by eLife

  eLife assessment

  This study presents evidence that suggests that the coalescence of sister chromatids induced by global double-strand DNA breaks (DSBs) during late mitosis is mediated by cohesin SMC3. These findings are valuable for studying the mechanism of eukaryotic cells to repair DNA during late mitosis. Although the discrete DSB induction system in budding yeast is sound, the strength of evidence is incomplete and could be buttressed to better support the major claims and to represent a clear advance with respect to the authors' previous contributions to this field.

Reviewed by eLife

Lactate transporter MCT1 in hepatic stellate cells promotes fibrotic collagen expression in nonalcoholic steatohepatitis

1. Kyounghee Min
2. Batuhan Yenilmez
3. Mark Kelly
4. Dimas Echeverria
5. Michael Elleby
6. Lawrence M Lifshitz
7. Naideline Raymond
8. Emmanouela Tsagkaraki
9. Shauna M Harney
10. Chloe DiMarzio
11. Hui Wang
12. Nicholas McHugh
13. Brianna Bramato
14. Brett Morrison
15. Jeffery D Rothstein
16. Anastasia Khvorova
17. Michael P Czech

• Curated by eLife

  eLife assessment

  This convincing manuscript represents a valuable advance in understanding the role of MCT1 – a transporter for lactate and other organic anions – in hepatocytes and hepatic stellate cells in the liver. The authors also generate exciting new tools to investigate hepatic stellate cell biology, and these may have much broader applications, but future studies are required to validate these new tools.

Reviewed by eLife

Cryo-EM structure of cell-free synthesized human histamine H ₂ receptor coupled to heterotrimeric G _s protein in lipid nanodisc environment

1. Zoe Köck
2. Kilian Schnelle
3. Margherita Persechino
4. Simon Umbach
5. Hannes Schihada
6. Dovile Januliene
7. Kristian Parey
8. Steffen Pockes
9. Peter Kolb
10. Volker Dötsch
11. Arne Möller
12. Daniel Hilger
13. Frank Bernhard

Reviewed by PREreview

KIS counteracts PTBP2 and regulates alternative exon usage in neurons

1. Marcos Moreno-Aguilera
2. Alba M Neher
3. Mónica B Mendoza
4. Martin Dodel
5. Faraz K Mardakheh
6. Raúl Ortiz
7. Carme Gallego

Reviewed by Review Commons

Gle1 is required for tRNA to stimulate Dbp5 ATPase activity in vitro and promote Dbp5-mediated tRNA export in vivo in Saccharomyces cerevisiae

1. Arvind Arul Nambi Rajan
2. Ryuta Asada
3. Ben Montpetit

• Curated by eLife

  eLife assessment

  The work is a valuable contribution to understanding the mechanism of nuclear export of tRNA in budding yeast. The authors present solid evidence that Dbp5 functions in parallel with Los1 and Msn5 in tRNA export, in a manner dependent on Gle1 for activation of its ATPase activity but independently of Mex67, Dbp5's partner in mRNA export. It further presents biochemical evidence that Dbp5 can bind tRNA but that Gle1 and InsP6 are required for activating ATP hydrolysis by the Dbp5-tRNA complex, suggesting a possible mechanism for tRNA export by Dbp5.

Reviewed by eLife

Role of the αC-β4 loop in protein kinase structure and dynamics

1. Jian Wu
2. Nisha A Jonniya
3. Sophia P Hirakis
4. Cristina Olivieri
5. Gianluigi Veglia
6. Alexandr P Kornev
7. Susan S Taylor

• Curated by eLife

  eLife assessment

  This valuable study draws attention to the importance of a previously overlooked structural motif in kinase regulation. While the data presented are intriguing and mostly solid, further analysis and additional experiments will be needed in the future to support the authors' hypothesis. The work will be of interest to protein biochemists and enzymologists with an interest in kinases and allostery.

Reviewed by eLife

Deep quantitative glycoproteomics reveals gut microbiome induced remodeling of the brain glycoproteome

1. Clément M Potel
2. Mira Lea Burtscher
3. Martin Garrido-Rodriguez
4. Amber Brauer-Nikonow
5. Isabelle Becher
6. Athanasios Typas
7. Michael Zimmermann
8. Mikhail M Savitski

Reviewed by PREreview

Adenine methylation is very scarce in the Drosophila genome and not erased by the ten-eleven translocation dioxygenase

1. Manon Boulet
2. Guerric Gilbert
3. Yoan Renaud
4. Martina Schmidt-Dengler
5. Emilie Plantié
6. Romane Bertrand
7. Xinsheng Nan
8. Tomasz Jurkowski
9. Mark Helm
10. Laurence Vandel
11. Lucas Waltzer

• Curated by eLife

  eLife assessment

  This study investigates the presence of DNA adenine methylation (6mA) and the associated function of TET enzyme, a DNA methylation mark eraser, in Drosophila. The study presents valuable findings on the scarcity of 6mA in the Drosophila genome and challenges previous findings regarding the role of TET in 6mA modification. The evidence supporting the claims is solid, and the paper has the potential to stimulate re-evaluations of the significance and regulatory mechanisms of 6mA DNA modifications in Drosophila.

Reviewed by eLife

A SUMO E3 ligase promotes long non-coding RNA transcription to regulate small RNA-directed DNA elimination

1. Salman Shehzada
2. Tomoko Noto
3. Julie Saksouk
4. Kazufumi Mochizuki

Reviewed by Review Commons

Conserved and repetitive motifs in an intrinsically disordered protein drive ⍺-carboxysome assembly

1. Julia B. Turnšek
2. Luke M. Oltrogge
3. David F. Savage

Reviewed by Arcadia Science