Molecular Biology

A fine kinetic balance of interactions directs transcription factor hubs to genes

1. Samantha Fallacaro
2. Apratim Mukherjee
3. Puttachai Ratchasanmuang
4. Joseph Zinski
5. Yara I Haloush
6. Kareena Shankta
7. Mustafa Mir

Reviewed by preLights

ACE2-independent sarbecovirus cell entry is supported by TMPRSS2-related enzymes and reduces sensitivity to antibody-mediated neutralization

1. Lu Zhang
2. Hsiu-Hsin Cheng
3. Nadine Krüger
4. Bojan Hörnich
5. Luise Graichen
6. Alexander S. Hahn
7. Sebastian R. Schulz
8. Hans-Martin Jäck
9. Metodi V. Stankov
10. Georg M.N. Behrens
11. Marcel A. Müller
12. Christian Drosten
13. Onnen Mörer
14. Martin Sebastian Winkler
15. ZhaoHui Qian
16. Stefan Pöhlmann
17. Markus Hoffmann

Reviewed by Review Commons

Multi-protein chimeric antigens, a novel combined approach for efficiently targeting and blocking the blood stage of Plasmodium falciparum

1. Bhagyashree Deshmukh
2. Dhruv Khatri
3. Sanjay Kumar Kochar
4. Chaitanya Athale
5. Krishanpal Karmodiya

Reviewed by Review Commons

Association with TFIIIC limits MYCN localisation in hubs of active promoters and chromatin accumulation of non-phosphorylated RNA polymerase II

1. Raphael Vidal
2. Eoin Leen
3. Steffi Herold
4. Mareike Müller
5. Daniel Fleischhauer
6. Christina Schülein-Völk
7. Dimitrios Papadopoulos
8. Isabelle Röschert
9. Leonie Uhl
10. Carsten P Ade
11. Peter Gallant
12. Richard Bayliss
13. Martin Eilers
14. Gabriele Büchel

• Curated by eLife

  eLife assessment

  This study presents the valuable finding that TFIIIC interacts with MYCN to regulate RNA polymerase II dynamics by dissecting its impact on 3D chromatin architecture. Authors provide convincing evidence that MYCN and TFIIIC show long-range chromatin contacts, and that the expression of each protein limits the function of the other. The notion emerges that TFIIIC helps MYCN to maintain output at promoters while decreasing less productive associations at larger more extensively connected chromatin hubs. The paper is of interest to molecular biologists working on MYCN-dependent regulation of gene expression.

Reviewed by eLife

Comprehensive Phenotyping of Extracellular Vesicles in Blood of Healthy Humans – Insights into Cellular Origin and Biological Variability

1. Marija Holcar
2. Ivica Marić
3. Tobias Tertel
4. Katja Goričar
5. Urška Čegovnik Primožič
6. Darko Černe
7. Bernd Giebel
8. Metka Lenassi

Reviewed by Arcadia Science

Deciphering the actin structure-dependent preferential cooperative binding of cofilin

1. Kien Xuan Ngo
2. Huong T Vu
3. Kenichi Umeda
4. Minh-Nhat Trinh
5. Noriyuki Kodera
6. Taro Uyeda

• Curated by eLife

  eLife assessment

  In this manuscript the authors present high-speed atomic force microscopy (HSAFM) to analyze real-time structural changes in actin filaments induced by cofilin binding. This important study enhances our understanding of actin dynamics which plays a crucial role in a broad spectrum of cellular activities based on solid experimental evidence. Some technical questions, however, remain, making the data interpretation incomplete.

Reviewed by eLife

circHIPK3 nucleates IGF2BP2 and functions as a competing endogenous RNA

1. Trine Line Hauge Okholm
2. Andreas Bjerregaard Kamstrup
3. Morten Muhlig Nielsen
4. Anne Kruse Hollensen
5. Mette Laugesen Graversgaard
6. Matilde Helbo Sørensen
7. Lasse Sommer Kristensen
8. Søren Vang
9. Samuel S Park
10. Eugene Yeo
11. Lars Dyrskjøt
12. Jørgen Kjems
13. Jakob Skou Pedersen
14. Christian Kroun Damgaard

• Curated by eLife

  eLife assessment

  This work explores the role of one the most abundant circRNAs, circHIPK3, in bladder cancer cells, showing with convincing data that circHIPK3 depletion affects thousands of genes and that those downregulated (including STAT3) share an 11-mer motif with circHIPK3, corresponding to a binding site for IGF2BP2. The experiments demonstrate that circHIPK3 can compete with the downregulated mRNAs targets for IGF2BP2 binding and that IGF2BP2 depletion antagonizes the effect of circHIPK3 depletion by upregulating the genes containing the 11-mer. These important findings contribute to the growing recognition of the complexity of cancer signaling regulation and highlight the intricate interplay between circRNAs and protein-coding genes in tumorigenesis.

Reviewed by eLife

Artificially inserted strong promoter containing multiple G-quadruplexes induces long-range chromatin modification

1. Shuvra Shekhar Roy
2. Sulochana Bagri
3. Soujanya Vinayagamurthy
4. Avik Sengupta
5. Claudia Regina Then
6. Rahul Kumar
7. Sriram Sridharan
8. Shantanu Chowdhury

• Curated by eLife

  eLife assessment

  This valuable study demonstrates that genomic insertion of a G4-containing sequence can be sufficient to induce chromosome loops and alter gene expression. The evidence supporting the conclusions is convincing. Effects were shown by Hi-C as well as qPCR for chromatin modifications and expression, and the specificity of the effects was controlled by mutating the G4-containing sequence or treating with LNA probes to abolish G4 structure formation. The work will be of interest to researchers working on chromatin organization and gene regulation.

Reviewed by eLife

Time-resolved mitochondrial-focused screening identifies regulatory components of oxidative metabolism

1. Marcos Zamora-Dorta
2. Sara Laine-Menéndez
3. David Abia
4. Pilar González-García
5. Luis C. López
6. Paula Fernández-Montes Díaz
7. Enrique Calvo
8. Jesús Vázquez
9. José Antonio Enríquez
10. Eduardo Balsa

Reviewed by Arcadia Science

Conserved regulatory motifs in the juxtamembrane domain and kinase N-lobe revealed through deep mutational scanning of the MET receptor tyrosine kinase domain

1. Gabriella O Estevam
2. Edmond M Linossi
3. Christian B Macdonald
4. Carla A Espinoza
5. Jennifer M Michaud
6. Willow Coyote-Maestas
7. Eric A Collisson
8. Natalia Jura
9. James S Fraser

• Curated by eLife

  eLife assessment

  This manuscript describes a deep mutational scanning study of the kinase domain of the MET receptor tyrosine kinase. The study yields an important catalog of essentially all possible deleterious mutations in this portion of the receptor., with convincing evidence. The manuscript will be of interest to researchers working in the field of receptor tyrosine kinases.

Reviewed by eLife