Molecular Biology

Diurnal control of iron responsive element containing mRNAs through iron regulatory proteins IRP1 and IRP2 is mediated by feeding rhythms

1. Hima Priyanka Nadimpalli
2. Georgia Katsioudi
3. Enes Salih Arpa
4. Lies Chikhaoui
5. Alaaddin Bulak Arpat
6. Angelica Liechti
7. Gaël Palais
8. Claudia Tessmer
9. Ilse Hofmann
10. Bruno Galy
11. David Gatfield

Reviewed by Review Commons

Molecular dependencies and genomic consequences of a global DNA damage tolerance defect

1. Daniel de Groot
2. Aldo Spanjaard
3. Ronak Shah
4. Maaike Kreft
5. Ben Morris
6. Cor Lieftink
7. Joyce J.I. Catsman
8. Shirley Ormel
9. Matilda Ayidah
10. Bas Pilzecker
11. Olimpia Alessandra Buoninfante
12. Paul C.M. van den Berk
13. Roderick L. Beijersbergen
14. Heinz Jacobs

Reviewed by Review Commons

Tead1 reciprocally regulates adult β-cell proliferation and function to maintain glucose homeostasis

1. Jeongkyung Lee
2. Ruya Liu
3. Byung S. Kim
4. Yiqun Zhang
5. Feng Li
6. Rajaganapti Jagannathan
7. Ping Yang
8. Vinny Negi
9. Joseph Danvers
10. Eliana Melissa Perez-Garcia
11. Pradip K. Saha
12. Omaima Sabek
13. Chad J. Creighton
14. Cristian Coarfa
15. Mark O. Huising
16. Hung-Ping Shih
17. Rita Bottino
18. Ke Ma
19. Mousumi Moulik
20. Vijay K. Yechoor

• Curated by eLife

  eLife assessment

  The Hippo signaling pathway plays a crucial role in controlling organ size, cell proliferation, and apoptosis, though its role in endocrine pancreas development has remained unclear. In this useful work, the authors study the function of the Tead1 transcription factor, a Hippo effector, specifically in pancreatic beta cells. They provide solid evidence, using multiple different conditional knockout models to reveal Tead1's regulatory functions in insulin secretion and beta cell proliferation. However, deeper exploration of their data and incorporating findings from existing literature on this topic would provide a clearer understanding of Tead1's role in β-cell function, within or beyond the Hippo pathway.

Reviewed by eLife

ChAHP2 and ChAHP control diverse retrotransposons by complementary activities

1. Josip Ahel
2. Aparna Pandey
3. Michaela Schwaiger
4. Fabio Mohn
5. Anja Basters
6. Georg Kempf
7. Aude Andriollo
8. Lucas Kaaij
9. Daniel Hess
10. Marc Bühler

Reviewed by Review Commons

Affinity-tagged SMAD1 and SMAD5 mouse lines reveal transcriptional reprogramming mechanisms during early pregnancy

1. Zian Liao
2. Suni Tang
3. Kaori Nozawa
4. Keisuke Shimada
5. Masahito Ikawa
6. Diana Monsivais
7. Martin Matzuk

• Curated by eLife

  eLife assessment

  This study presents two valuable new mouse models that individually tag proteins from the SMAD family to identify distinct roles during early pregnancy. Convincing evidence is provided that SMAD1 and SMAD5 target many of the same genomic regions as each other and the progesterone receptor. Given the broad effect of these signaling pathways in multiple systems, these new tools will most likely interest readers across biological disciplines.

Reviewed by eLife

Proteolytic cleavage and inactivation of the TRMT1 tRNA modification enzyme by SARS-CoV-2 main protease

1. Kejia Zhang
2. Patrick Eldin
3. Jessica H Ciesla
4. Laurence Briant
5. Jenna M Lentini
6. Jillian Ramos
7. Justin Cobb
8. Joshua Munger
9. Dragony Fu

• Curated by eLife

  eLife assessment

  This manuscript provides important insights into the degradation of the host tRNA modification enzyme TRMT1 by the SARS-CoV-2 protease Nsp5 (nonstructural protein 5 or MPro). The data convincingly support the main conclusions of the paper. These results will be of interest to virologists studying the alterations in tRNA modifications, host methyltransferases, and viral infections.

Reviewed by eLife

Synthetic type III-E CRISPR-Cas effectors for programmable RNA-targeting

1. Daniel J. Brogan
2. Elena Dalla Benetta
3. Tianqi Wang
4. Calvin P. Lin
5. Fangying Chen
6. Harry Li
7. Claire Lin
8. Elizabeth A. Komives
9. Omar S. Akbari

Reviewed by Arcadia Science

Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenation

1. Wayne Mitchell
2. Ludger JE Goeminne
3. Alexander Tyshkovskiy
4. Sirui Zhang
5. Julie Y Chen
6. Joao A Paulo
7. Kerry A Pierce
8. Angelina H Choy
9. Clary B Clish
10. Steven P Gygi
11. Vadim N Gladyshev

• Curated by eLife

  eLife assessment

  This important study reports comprehensive multi-omics data on the changes induced in young and aged male mouse tail fibroblasts after treatment with chemical reprogramming factors. The authors provide solid evidence to support their claim that chemical reprogramming factors induce changes consistent with a reduction of cellular 'biological' age (e.g., correlations with established aging markers in whole tissues).

Reviewed by eLife

Sperm fertility in mice with oligo-astheno-teratozoospermia restored by in vivo injection and electroporation of naked mRNA

1. Charline Vilpreux
2. Guillaume Martinez
3. Paul Fourquin
4. Magali Court
5. Florence Appaix
6. Jean-Luc Duteyrat
7. Maxime Henry
8. Julien Vollaire
9. Camille Ayad
10. Altan Yavuz
11. Lisa De Macedo
12. Geneviève Chevalier
13. Edgar Del Llano
14. Emeline Lambert
15. Sekou Ahmed Conte
16. Zeina Wehbe
17. Elsa Giordani
18. Véronique Josserand
19. Jacques Brocard
20. Coutton Charles
21. Bernard Verrier
22. Pierre F. Ray
23. Corinne Loeuillet
24. Christophe Arnoult
25. Jessica Escoffier

• Curated by eLife

  eLife assessment

  This potentially useful study reports a new method for restoring sperm motility. Strengths are in the methodology being developed, but the conclusions require additional experimental support. The authors provide inadequate evidence for the success of the method or its mechanism.

Reviewed by eLife

A myristoyl switch at the plasma membrane triggers cleavage and oligomerization of Mason-Pfizer monkey virus matrix protein

1. Markéta Častorálová
2. Jakub Sýs
3. Jan Prchal
4. Anna Pavlů
5. Lucie Prokopová
6. Zina Briki
7. Martin Hubálek
8. Tomas Ruml

• Curated by eLife

  eLife assessment

  This valuable study advances our understanding of how the viral protease in a D-type retrovirus is activated and in particular how the exposure of the myristoyl group is required for processing of the Gag matrix precursor. The supporting evidence is convincing, but the work would benefit from additional data in support of the claims. This manuscript is of interest to retrovirologists and structural biologists.

Reviewed by eLife