Molecular Biology

Dietary sulfur amino acid restriction elicits a cold-like transcriptional response in inguinal but not epididymal white adipose tissue of male mice

1. Philip MM Ruppert
2. Aylin S Güller
3. Marcus Rosendal
4. Natasa Stanic
5. Jan-Wilhelm Kornfeld

• Curated by eLife

  eLife Assessment

  The present study employed transcriptomics to investigate the impact of methionine restriction (MR) and cold exposure (CE) on liver and adipose tissues in mice. The authors demonstrate that responses to MR and CE are tissue-specific, while both MR and CE have a similar effect on beige adipose tissue. While these findings are somewhat descriptive, this work is considered important, as it provides a comprehensive resource for enhancing our understanding of these lifestyle interventions. The study is of high scientific quality, and the analyses are convincing.

Reviewed by eLife

Proximity labeling reveals ZFP36L1 as a central hub for post-transcriptional regulation networks in T cells

1. Anouk P. Jurgens
2. Branka Popović
3. Floris P.J. van Alphen
4. Leyma Wardak
5. Antonia Bradarić
6. Sander Engels
7. Carmen van der Zwaan
8. Maartje van den Biggelaar
9. Arie J. Hoogendijk
10. Julien Béthune
11. Monika C. Wolkers

Reviewed by Review Commons

NASP functions in the cytoplasm to prevent histone H3 aggregation during early embryogenesis

1. Mohit Das
2. Eli Coronado-Chavez
3. Anusha D Bhatt
4. Reyhaneh Tirgar
5. Amanda A Amodeo
6. Jared Nordman

Reviewed by Review Commons

Inhibiting Glutamine Metabolism Blocks Coronavirus Replication in Mammalian Cells

1. Kai Su Greene
2. Annette Choi
3. Matthew Chen
4. Nianhui Yang
5. Ruizhi Li
6. Yijian Qiu
7. Michael J Lukey
8. Katherine S Rojas
9. Jonathan Shen
10. Kristin F Wilson
11. William P Katt
12. Gary R Whittaker
13. Richard A Cerione

Reviewed by Rapid Reviews Infectious Diseases

Malaria parasite resistance to azithromycin is not readily transmitted by mosquitoes

1. Hayley D. Buchanan
2. Robyn McConville
3. Lee M. Yeoh
4. Michael F. Duffy
5. Justin A. Boddey
6. Geoffrey I. McFadden
7. Christopher D. Goodman

• Curated by eLife

  eLife assessment

  This important work addressed the identifcation of antimalarial drug resistance mutations that do not readily transmit to new human hosts, focusing on azithromycin resistance. The technically challenging analyses of azithromycin-resistant parasites as they traverse the mosquito host and human liver are conducted using state-of-the-art tools, including humanized mice. While the claim regarding the lack of transmission by atovaquone-resistant P. berghei is convincing, the evidence for the lack of transmission by atovaquone-resistant P. falciparum is insufficient. This work will appeal to biologists and biomedical scientists in parasitology and drug discovery, offering insights into combating antimalarial drug resistance and understanding the fitness costs associated with drug-resistant parasites.

Reviewed by eLife, Rapid Reviews Infectious Diseases

The chromatin remodeller CHD4 regulates transcription factor binding to both prevent activation of silent enhancers and maintain active regulatory elements

1. Andria Koulle
2. Oluwaseun Ogundele
3. Devina Shah
4. India-May Baker
5. Maya Lopez
6. Nicola Reynolds
7. Ramy Ragheb
8. Ernest Laue
9. Brian Hendrich

• Curated by eLife

  eLife Assessment

  This work offers important insights into the protein CHD4's function in chromatin remodeling and gene regulation in embryonic stem cells, supported by extensive biochemical, genomic, and imaging data. The use of an inducible degron system allows precise functional analysis, and the datasets generated represent a key resource for the field. While some interpretations of complex data could be more strongly substantiated, the study overall provides compelling evidence and makes a significant contribution to understanding CHD4's role in epigenetic regulation. This work will be of interest to the epigenetics and stem biology fields.

Reviewed by eLife

Interplay between cohesin and TORC1 links chromosome segregation and gene expression to environmental changes

1. Dorian Besson
2. Sabine Vaur
3. Stéphanie Vazquez
4. Sylvie Tournier
5. Yannick Gachet
6. Adrien Birot
7. Stéphane Claverol
8. Adèle Marston
9. Anastasios Damdimopoulos
10. Karl Ekwall
11. Jean-Paul Javerzat

• Curated by eLife

  eLife Assessment

  This important study describes a new link between nutrient signaling and chromosome regulation, providing compelling evidence that reduced activity in the central nutrient-sensing pathway governed by TORC1 improves chromosome stability and alters gene expression in S. pombe through effects on cohesin. While the biological importance of this newly described circuit is not yet fully known, and some data would benefit from further clarification, the overall body of evidence supports the main conclusions.

Reviewed by eLife

Single cell transcriptomics and development of gametocyte-specific molecular markers for avian malaria parasites

1. A Berthomieu
2. S Gandon
3. AM Talman
4. A Rivero

Reviewed by PREreview

Mechanistic insights into transcriptional regulation of ARHGAP36 expression identify a factor predictive of neuroblastoma survival

1. Serhiy Havrylov
2. Armin M Gamper
3. Ordan J Lehmann

• Curated by eLife

  eLife Assessment

  This important study examines the potential role of ARHGAP36 transcriptional regulation by FOXC1 in controlling sonic hedgehog signaling in human neuroblastoma. While there are many solid findings that strongly support this signaling pathway, there are some aspects of the study that are underdeveloped.

Reviewed by eLife

Insights from aquaporin structures into drug-resistant sleeping sickness

1. Modestas Matusevicius
2. Robin A Corey
3. Marcos Gragera
4. Keitaro Yamashita
5. Teresa Sprenger
6. Marzuq A Ungogo
7. James N Blaza
8. Pablo Castro-Hartmann
9. Dimitri Y Chirgadze
10. Sundeep Chaitanya Vedithi
11. Pavel Afanasyev
12. Roberto Melero
13. Rangana Warshamanage
14. Anastasiia Gusach
15. Jose Maria Carazo
16. Mark Carrington
17. Tom L Blundell
18. Garib Murshudov
19. Phillip Stansfeld
20. Mark Sansom
21. Harry P de Koning
22. Christopher G Tate
23. Simone N Weyand

• Curated by eLife

  eLife Assessment

  In this important study, the authors set out to determine the molecular interactions between the AQP2 from Trypanosoma brucei (TbAQP2) and the trypanocidal drugs pentamidine and melarsoprol to understand how TbAQP2 mutations lead to drug resistance. Using cryo-EM, molecular dynamics simulations, and lysis assays the authors present convincing evidence that mutations in TbAQP2 make permeation of trypanocidal drugs energetically less favourable, and that this impacts the ability of drugs to achieve a therapeutic dose. Overall, this data will be of interest for those working on aquaporins, and development of trypanosomiasis drugs as well as drugs targeting aquaporins in general.

Reviewed by eLife