1. Human eIF2A has a minimal role in translation initiation and in uORF-mediated translational control in HeLa cells

    This article has 3 authors:
    1. Mykola Roiuk
    2. Marilena Neff
    3. Aurelio A. Teleman
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      In this valuable study, Roiuk et al combined ribosome profiling and reporter assays to provide compelling evidence that eIF2A does not have a major impact on mRNA translation in HeLa cells. These findings are consistent with several recent publications that disaffirm the previously proposed role of eIF2A in directing protein synthesis under stress. Considering that stress-dependent perturbations in translation play a major role in homeostasis and several pathological states (e.g., cancer and neurological disorders), this work should be of broad interest to researchers studying regulation of gene expression, stress-adaptation, cancer and neurobiology.

    Reviewed by eLife

    This article has 12 evaluationsAppears in 1 listLatest version Latest activity
  2. The Molecular Logic of Gtr1/2 and Pib2 Dependent TORC1 Regulation in Budding Yeast

    This article has 6 authors:
    1. Jacob H. Cecil
    2. Cristina M. Padilla
    3. Austin A. Lipinski
    4. Paul R. Langlais
    5. Xiangxia Luo
    6. Andrew P. Capaldi
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The study presents valuable findings concerning how a highly conserved signal transduction pathway helps budding yeast cells adapt their growth to nitrogen sources of differing qualities. However, the evidence is incomplete for the authors' main claim that the pathway adopts three distinct states depending on the nitrogen source. The presented data, particularly phospho-proteomic datasets, will be of interest to the cell growth signaling community.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  3. A platform for lab management, note-keeping and automation

    This article has 3 authors:
    1. Aubin Fleiss
    2. Alexander S. Mishin
    3. Karen S. Sarkisyan

    Reviewed by Arcadia Science

    This article has 1 evaluationAppears in 1 listLatest version Latest activity
  4. Nucleoplasmic Lamin A/C controls replication fork restart upon stress by modulating local H3K9me3 and ADP-ribosylation levels

    This article has 11 authors:
    1. Veronica Cherdyntseva
    2. Joanna Paulson
    3. Selin Adakli
    4. Jean-Philippe Gagné
    5. Moses Aouami
    6. Patricia Ubieto-Capella
    7. Daniel González-Acosta
    8. Collin Bakker
    9. Guy G. Poirier
    10. Nitika Taneja
    11. Massimo Lopes

    Reviewed by preLights

    This article has 1 evaluationAppears in 1 listLatest version Latest activity
  5. NF-κB is a Central Regulator of Hypoxia-Induced Gene Expression

    This article has 6 authors:
    1. Dilem Shakir
    2. Michael Batie
    3. Chun-Sui Kwok
    4. Simon J. Cook
    5. Niall S. Kenneth
    6. Sonia Rocha

    Reviewed by Review Commons

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  6. RNase III cleavage signals spread across splice junctions enforce sequential processing of co-hosted snoRNAs

    This article has 7 authors:
    1. Valérie Migeot
    2. Yves Mary
    3. Etienne Fafard-Couture
    4. Pierre Lombard
    5. François Bachand
    6. Michelle S Scott
    7. Carlo Yague-Sanz

    Reviewed by Review Commons

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  7. Negative regulation of miRNAs sorting in EVs: the RNA-binding protein PCBP2 impairs SYNCRIP-mediated miRNAs EVs loading

    This article has 14 authors:
    1. Francesco Marocco
    2. Sabrina Garbo
    3. Claudia Montaldo
    4. Alessio Colantoni
    5. Luca Quattrocchi
    6. Gioele Gaboardi
    7. Giovanna Sabarese
    8. Carla Cicchini
    9. Mario Lecce
    10. Alessia Carnevale
    11. Rossella Paolini
    12. Gian Gaetano Tartaglia
    13. Cecilia Battistelli
    14. Marco Tripodi
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This manuscript makes a valuable contribution to the field by uncovering a molecular mechanism for miRNA intracellular retention, mediated by the interaction of PCBP2, SYNCRIP, and specific miRNA motifs. The findings are convincing and advance our understanding of RNA-binding protein-mediated miRNA sorting, providing deeper insights into miRNA dynamics.

    Reviewed by eLife

    This article has 11 evaluationsAppears in 1 listLatest version Latest activity
  8. Structure of an oxygen-induced tubular nanocompartment in Pyrococcus furiosus

    This article has 11 authors:
    1. Wenfei Song
    2. Jan Fiala
    3. Ioannis Skalidis
    4. Pascal Albanese
    5. Constantinos Patinios
    6. Marten L Chaillet
    7. Servé WM Kengen
    8. Richard A Scheltema
    9. Stuart C Howes
    10. Albert JR Heck
    11. Friedrich Förster
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      Using advanced CryoEM and mass spectrometry, the authors provide compelling evidence of how tubule formation occurs in an oxygen-dependent manner. These fundamental findings offer a novel mechanism by which rubrerythrin tubules encapsulate encapsulin to prevent oxidative stress in Pyrococcus furiosus. However, there are a few reasonable concerns about biochemical validations and the lack of adequate description of results and methodology.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  9. Synonymous mutations in AAV Rep enhance genome packaging in a library selection

    This article has 3 authors:
    1. Tasfia Azim
    2. Dru Myerscough
    3. Jonathan J Silberg
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      Engineering of adeno-associated virus (AAV) replication proteins may provide new insights into Parvoviral replication. The authors created a useful collection of Rep protein variants with changes that alter the amino acid sequence, but these did not lead to clear improvements in how the virus worked. Instead, their screen showed that changes that do not alter the protein ("synonymous" mutations) and changes to the promoter were more common. As it stands the results are incomplete due to potential issues with the screening design. We encourage a more complete characterization, which may enhance the translational potential of the approach.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  10. PI3Kα inhibition blocks osteochondroprogenitor specification and the hyper-inflammatory response to prevent heterotopic ossification

    This article has 8 authors:
    1. José Antonio Valer
    2. Alexandre Deber
    3. Marius Wits
    4. Carolina Pimenta-Lopes
    5. Marie-José Goumans
    6. José Luis Rosa
    7. Gonzalo Sánchez-Duffhues
    8. Francesc Ventura
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study, which includes additional experiments in response to the reviewer comments, presents valuable findings illustrating the role of PI3Kα in heterotopic ossification in FOP model mice. The methods, data, and analyses are solid and generally support the claims although as noted by one of the reviewers, there is no data demonstrating the effect of BYL79 on cell growth, and it remains unclear whether BYL79 also inhibits the Smad2/3 pathway. While this study provides new insights into the role of the PI3Kα pathway as a therapeutic target for FOP, questions about the mechanism of BYL79 still exist.

    Reviewed by eLife

    This article has 11 evaluationsAppears in 1 listLatest version Latest activity
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