Microbiology

WhyD tailors surface polymers to prevent premature bacteriolysis and direct cell elongation in Streptococcus pneumoniae

1. Josué Flores-Kim
2. Genevieve S Dobihal
3. Thomas G Bernhardt
4. David Z Rudner

• Curated by eLife

  Evaluation Summary:

  This study explores autolysis regulation in the gram positive pathogen Streptococcus Pneumoniae and finds that the turnover of wall teichoic acids at the septum controls cell elongation. Specifically, a possible new enzyme WhyD regulates the level of wall teichoic acids (WTA) at the bacterial septum, limiting the number of WTA-associated autolysins such as LytA at division sites. The study provides new insights into the molecular interactions between enzymes that regulate PG-associated polymers and enzymes that hydrolyze to regulate cell morphogenesis. In principle, such regulation will also apply to other monoderm bacteria. This article will be of interest to microbiologists who are studying bacterial cell envelope dynamics. The experimental data support the conclusions drawn and the work provides tools, concepts and remaining questions to address that will be instrumental to understand the mechanism underpinning autolytic control.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Near-native state imaging by cryo-soft-X-ray tomography reveals remodelling of multiple cellular organelles during HSV-1 infection

1. Kamal L. Nahas
2. Viv Connor
3. Katharina M. Scherer
4. Clemens F. Kaminski
5. Maria Harkiolaki
6. Colin M. Crump
7. Stephen C. Graham

Reviewed by Review Commons

The SARS-CoV-2 Cellular Receptor ACE2 is expressed in Oropharyngeal Cells and is modulated in vitro by the Bacterial Lysate Lantigen B

1. Caterina Pizzimenti
2. Antonella D’Agostino
3. Paola Pirrello
4. Alessia Ruiba
5. Giovanni Melioli

Reviewed by ScreenIT

Cell cycle independent role of cyclin D3 in host restriction of SARS-CoV-2 infection

1. Ravi K. Gupta
2. Petra Mlcochova

Reviewed by ScreenIT

ORF6 protein of SARS-CoV-2 inhibits TRIM25 mediated RIG-I ubiquitination to mitigate type I IFN induction

1. Oyahida Khatun
2. Mansi Sharma
3. Rohan Narayan
4. Shashank Tripathi

Reviewed by ScreenIT

SARS-CoV-2 Evolution and Patient Immunological History Shape the Breadth and Potency of Antibody-Mediated Immunity

1. Maria Manali
2. Laura A Bissett
3. Julien A R Amat
4. Nicola Logan
5. Sam Scott
6. Ellen C Hughes
7. William T Harvey
8. Richard Orton
9. Emma C Thomson
10. Rory N Gunson
11. Mafalda Viana
12. Brian Willett
13. Pablo R Murcia

Reviewed by ScreenIT

Reaction Conditions Promoting the Specific Detection of SARS-CoV-2 NendoU Enzymatic Activity

1. Nodar Makharashvili
2. James O. McNamara

Reviewed by ScreenIT

SARS-CoV-2 Omicron subvariants evolved to promote further escape from MHC-I recognition

1. Miyu Moriyama
2. Carolina Lucas
3. Valter Silva Monteiro
4. Yale SARS-CoV-2 Genomic Surveillance Initiative
5. Akiko Iwasaki

Reviewed by ScreenIT

Neutralisation sensitivity of SARS-CoV-2 omicron subvariants to therapeutic monoclonal antibodies

1. Daichi Yamasoba
2. Yusuke Kosugi
3. Izumi Kimura
4. Shigeru Fujita
5. Keiya Uriu
6. Jumpei Ito
7. Kei Sato

Reviewed by ScreenIT

Soluble MAC is primarily released from MAC-resistant bacteria that potently convert complement component C5

1. Dennis J Doorduijn
2. Marie V Lukassen
3. Marije FL van 't Wout
4. Vojtech Franc
5. Maartje Ruyken
6. Bart W Bardoel
7. Albert JR Heck
8. Suzan HM Rooijakkers

• Curated by eLife

  Evaluation Summary:

  The soluble membrane attack complex (sMAC) is generated from complement activation and contains the complement proteins C5b, C6, C7, C8, C9 together with the regulatory proteins clusterin and vitronectin. Despite intense interest in sMAC, the mechanisms regulating its formation remain poorly understood. In this manuscript the authors demonstrate that sMAC is formed when complement is activated on bacteria that are resistant to killing by MAC pores.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife