Microbiology

PfHDAC1 is an essential regulator of parasite asexual growth with its altered genomic occupancy and activity associated with artemisinin drug resistance in Plasmodium falciparum

1. Abhishek Kanyal
2. Heledd Davies
3. Bhagyashree Deshmukh
4. Dilsha Farheen
5. Moritz Treeck
6. Krishanpal Karmodiya

Reviewed by Review Commons

Chemical Links Between Redox Conditions and Estimated Community Proteomes from 16S rRNA and Reference Protein Sequences

1. Jeffrey M. Dick
2. Jingqiang Tan

• Curated by eLife

  Evaluation Summary:

  This study presents an in-depth analyses of carbon oxidation state and hydration state of proteomes in different taxa and environmental settings, which contributes to our understanding of how microbial communities are shaped by their surroundings. The study has merit, but there also some technical weaknesses.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Legionella‐ and host‐driven lipid flux at LCV‐ER membrane contact sites promotes vacuole remodeling

1. Simone Vormittag
2. Dario Hüsler
3. Ina Haneburger
4. Tobias Kroniger
5. Aby Anand
6. Manuel Prantl
7. Caroline Barisch
8. Sandra Maaß
9. Dörte Becher
10. François Letourneur
11. Hubert Hilbi

Reviewed by Review Commons

Developmental Transitions Coordinate Assembly of the Coxiella burnetii Dot/Icm Type IV Secretion System

1. Donghyun Park
2. Samuel Steiner
3. Meng Shao
4. Craig R. Roy
5. Jun Liu

• Curated by eLife

  Evaluation Summary:

  Park et al.'s work provides insight into the infection processes of the human pathogen Coxiella burnetii with unprecedented detail. Their time course of cellular infection reveals the timing of key events and detects a previously unrecognized membrane structure. This work will shed new insight into the infection process of this pathogen allowing new targets for the treatment of infection with Coxiella.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

An essential periplasmic protein coordinates lipid trafficking and is required for asymmetric polar growth in mycobacteria

1. Kuldeepkumar R Gupta
2. Celena M Gwin
3. Kathryn C Rahlwes
4. Kyle J Biegas
5. Chunyan Wang
6. Jin Ho Park
7. Jun Liu
8. Benjamin M Swarts
9. Yasu S Morita
10. E Hesper Rego

• Curated by eLife

  Evaluation Summary:

  This manuscript tackles the important question of what proteins regulate asymmetrical cell division in Mycobacteria. This will be of interest to all individuals interested in bacterial physiology. The data are sound, but some of the conclusions need to be tempered or bolstered, in relation to the models proposed.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

A positive feedback loop mediates crosstalk between calcium, cyclic nucleotide and lipid signalling in calcium-induced Toxoplasma gondii egress

1. Stephanie D. Nofal
2. Caia Dominicus
3. Malgorzata Broncel
4. Nicholas J. Katris
5. Helen R. Flynn
6. Gustavo Arrizabalaga
7. Cyrille Y. Botté
8. Brandon M. Invergo
9. Moritz Treeck

Reviewed by Review Commons

Human neutrophil IL1β directs intestinal epithelial cell extrusion during Salmonella infection

1. Anna-Lisa E. Lawrence
2. Ryan P. Berger
3. David R. Hill
4. Sha Huang
5. Veda K. Yadagiri
6. Brooke Bons
7. Courtney Fields
8. Gautam J. Sule
9. Jason S. Knight
10. Christiane E. Wobus
11. Jason R. Spence
12. Vincent B. Young
13. Mary X. O’Riordan
14. Basel H. Abuaita

Reviewed by Review Commons

Dissecting the invasion of Galleria mellonella by Yersinia enterocolitica reveals metabolic adaptations and a role of a phage lysis cassette in insect killing

1. Philipp-Albert Sänger
2. Stefanie Wagner
3. Elisabeth M. Liebler-Tenorio
4. Thilo M. Fuchs

Reviewed by Review Commons

Convergence of two global regulators to coordinate expression of essential virulence determinants of Mycobacterium tuberculosis

1. Hina Khan
2. Partha Paul
3. Ritesh Rajesh Sevalkar
4. Sangita Kachhap
5. Balvinder Singh
6. Dibyendu Sarkar

• Curated by eLife

  Evaluation Summary:

  This paper will be of broad interest to those working on the regulation of gene expression and mycobacteria as it deals with the collaboration of two important transcription regulators. A combination of experiments indicates how a complex of two regulators selectively turns on gene expression of a few genes in intracellular pathogen Mycobacterium tuberculosis.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Temporal and thermal profiling of the Toxoplasma proteome implicates parasite Protein Phosphatase 1 in the regulation of Ca2+-responsive pathways

1. Alice L Herneisen
2. Zhu-Hong Li
3. Alex W Chan
4. Silvia NJ Moreno
5. Sebastian Lourido

• Curated by eLife

  Evaluation Summary:

  Herneisen et al provide a comprehensive and thorough exploration of Ca2+ responsive changes in the Toxoplasma proteome and the resulting phosphorylation events during the transition from intracellular residing parasites to egress from the host cell. Furthermore, a novel temperature stability profiling method of all proteins responding to Ca2+ concentration with a change in stability is a novel applicable tool that here is used to map Ca2+-responsive proteins in the parasites. They provide a compelling analysis of the complex data and carefully validate their findings using genetics and cell biology. This work is of the highest quality in the field.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife