Herd immunity on chip: recapitulating virus transmission in human society

Article activity feed

1. 

   ScreenIT

   Jun 2, 2022

   SciScore for 10.1101/2022.05.27.493795: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   NIH rigor criteria are not applicable to paper type.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   MRC-5 cells labeled with green fluorescent protein (MRC-5 GFP) were purchased from Lugen Sci Co., Ltd. (Bucheon, Korea).	MRC-5 GFP suggested: None
   MRC-5 cells were maintained in minimum essential media (MEM) (HyClone Laboratories, Logan, UT) supplemented with 10% (v/v	MRC-5 suggested: None
   In brief, freshly cultured MRC-5 or Hu7 cells (104 cells per well) were seeded in a 96-well plate (Corning) and incubated at 37 °C under 5% CO2 overnight.	Hu7 suggested: None
   To generate unsusceptible cells (U0), MRC-5 or Huh-7 was transfected with either aminopeptidase (APN) small interfering RNA …

   SciScore for 10.1101/2022.05.27.493795: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   NIH rigor criteria are not applicable to paper type.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   MRC-5 cells labeled with green fluorescent protein (MRC-5 GFP) were purchased from Lugen Sci Co., Ltd. (Bucheon, Korea).	MRC-5 GFP suggested: None
   MRC-5 cells were maintained in minimum essential media (MEM) (HyClone Laboratories, Logan, UT) supplemented with 10% (v/v	MRC-5 suggested: None
   In brief, freshly cultured MRC-5 or Hu7 cells (104 cells per well) were seeded in a 96-well plate (Corning) and incubated at 37 °C under 5% CO2 overnight.	Hu7 suggested: None
   To generate unsusceptible cells (U0), MRC-5 or Huh-7 was transfected with either aminopeptidase (APN) small interfering RNA (siRNA) oligonucleotide (sense: 5’-GCA GCA GAU CUG UAU AUU U-3’, antisense: 5’-AAA UAU ACA GAU CUG C-3’) or negative control siRNA from Bioneer Co. (Daejeon, Korea).	Huh-7 suggested: CLS Cat# 300156/p7178_HuH7, RRID:CVCL_0336)
   Software and Algorithms
   Sentences	Resources
   Images were processed using Image J (NIH, Bethesda,	Image J suggested: (ImageJ, RRID:SCR_003070)

   ---

   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

   ---

   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

   ---

   Results from TrialIdentifier: No clinical trial numbers were referenced.

   ---

   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

   ---

   Results from JetFighter: We did not find any issues relating to colormaps.

   ---

   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

   ---

   Results from scite Reference Check: We found no unreliable references.

   ---

   Read the original source
2. 

   Version published to 10.1101/2022.05.27.493795 on bioRxiv

   May 30, 2022