Microbiology

Machine learning approaches identify immunologic signatures of total and intact HIV DNA during long-term antiretroviral therapy

1. Lesia Semenova
2. Yingfan Wang
3. Shane Falcinelli
4. Nancie Archin
5. Alicia D Cooper-Volkheimer
6. David M Margolis
7. Nilu Goonetilleke
8. David M Murdoch
9. Cynthia D Rudin
10. Edward P Browne

• Curated by eLife

  eLife assessment

  Semenova et al. have studied a large cross-sectional cohort of people living with HIV on suppressive antiretroviral therapy and performed high dimensional flow-cytometry for analysis with data science/machine learning approaches to investigate associations of immunological and clinical parameters and intact/total HIV DNA levels (and categorizations). The study is useful in introducing these new methods and large data set and appears mostly solid, though some of the claims were incompletely supported by the modeling results. The authors have revised the text to fairly reflect their results, yet open questions remain about utility, particularly as to the value of categorical classification (vs continuous measurement) of reservoir size.

Reviewed by eLife

Competition and cooperation: The plasticity of bacteria interactions across environments

1. Josephine Solowiej-Wedderburn
2. Jennifer T. Pentz
3. Ludvig Lizana
4. Björn Schröder
5. Peter Lind
6. Eric Libby

Reviewed by Arcadia Science

Secondary metabolite profiling of Pseudomonas aeruginosa isolates reveals rare genomic traits

1. Rachel L. Neve
2. Emily Giedraitis
3. Madeline S. Akbari
4. Shirli Cohen
5. Vanessa V. Phelan

Reviewed by Arcadia Science

Functional genomics screens reveal a role for TBC1D24 and SV2B in antibody-dependent enhancement of dengue virus infection

1. Laura Belmont
2. Maya Contreras
3. Catiana H. Cartwright-Acar
4. Caleb D. Marceau
5. Aditi Agrawal
6. Lisa M. Levoir
7. Jay Lubow
8. Leslie Goo

Reviewed by Rapid Reviews Infectious Diseases

Polysaccharides induce deep-sea Lentisphaerae strains to release chronic bacteriophages

1. Chong Wang
2. Rikuan Zheng
3. Tianhang Zhang
4. Chaomin Sun

• Curated by eLife

  eLife assessment

  This manuscript presents valuable findings on two isolates of deep sea Lentisphaerae strains, which further our understanding of deep sea microbial life. The manuscript's primary claim is that phage isolates augment polysaccharide use in Pseudomonas bacteria, with preliminary evidence for the potential auxiliary metabolic genes in chronic phage infection and/or host proliferation. The strength of the evidence is overall solid and there are only minor weaknesses regarding the mechanism of polysaccharide use by the phages and the evidence for chronic infection. Overall, the data on Lentisphaerae strains will deepen our understanding of microbial life in the deep sea.

Reviewed by eLife

Antibacterial activity of tamoxifen derivatives against methicillin-resistant Staphylococcus aureus

1. Irene Molina Panadero
2. Javier Falcón Torres
3. Karim Hmadcha
4. Salvatore Princiotto
5. Luigi Cutarella
6. Mattia Mori
7. Sabrina Dallavalle
8. Michael S. Christodoulou
9. Younes Smani

Reviewed by PREreview

BiP/GRP78 is a pro-viral factor for diverse dsDNA viruses that promotes the survival and proliferation of cells upon KSHV infection

1. Guillermo Najarro
2. Kevin Brackett
3. Hunter Woosley
4. Leah C. Dorman
5. Vincent Turon-Lagot
6. Sudip Khadka
7. Catya Faeldonea
8. Osvaldo Kevin Moreno
9. Adriana Ramirez Negron
10. Christina Love
11. Ryan Ward
12. Charles Langelier
13. Frank McCarthy
14. Carlos Gonzalez
15. Joshua E. Elias
16. Brooke M. Gardner
17. Carolina Arias

Reviewed by Review Commons

Non-cognate immunity proteins provide broader defenses against interbacterial effectors in microbial communities

1. Abigail Knecht
2. Denise Sirias
3. Daniel R Utter
4. Karine A Gibbs

• Curated by eLife

  eLife assessment

  This study provides valuable insights into the specificity and promiscuity of toxic effector and immunity protein pairs. While the work is improved over a previous version, there are still some questions regarding the methodology used to draw certain conclusions, rendering the study somewhat incomplete. Nevertheless, this work will likely be of interest to microbiologists and biochemists working with toxin-antitoxin systems and effector-immunity proteins.

Reviewed by eLife

Polysaccharide breakdown products drive degradation-dispersal cycles of foraging bacteria through changes in metabolism and motility

1. Astrid Katharina Maria Stubbusch
2. Johannes M Keegstra
3. Julia Schwartzman
4. Sammy Pontrelli
5. Estelle E Clerc
6. Samuel Charlton
7. Roman Stocker
8. Cara Magnabosco
9. Olga T Schubert
10. Martin Ackermann
11. Glen G D'Souza

• Curated by eLife

  eLife assessment

  This manuscript is a valuable contribution to our understanding of foraging behaviors in marine bacteria. The authors present a conceptual model for how a marine bacterial species consumes an abundant polysaccharide. Using experiments in microfluidic devices and through measurements of motility and gene expression, the authors offer convincing evidence that the degradation products of polysaccharide digestion can stimulate motility.

Reviewed by eLife

Mitochondrial ATP synthesis is essential for efficient gametogenesis in Plasmodium falciparum

1. Penny C Sparkes
2. Mufuliat Toyin Famodimu
3. Eduardo Alves
4. Eric Springer
5. Jude Przyborski
6. Michael J Delves

Reviewed by PREreview