Microbiology

Reversion to sensitivity explains limited transmission of resistance in a hospital pathogen

1. Kevin C Tracy
2. Jordan McKaig
3. Clare Kinnear
4. Jess Millar
5. Aaron A King
6. Andrew F Read
7. Robert J Woods

• Curated by eLife

  eLife Assessment

  This important study, which will be of interest to those studying the evolution and maintenance of antibiotic resistance, addresses the hypothesis that antibiotic resistance arising de novo during treatment will carry a higher fitness cost and will revert to susceptibility more readily than resistance that has been transmitted between hosts. There are, however, concerns that the 'putatively transmitted isolates' in this study do not necessarily represent resistant isolates that have been transmitted between hosts. The support for the central claim of different patterns of reversion between isolates with de novo resistance and putatively transmitted resistant isolates is currently incomplete.

Reviewed by eLife

Trim7 does not have a role in the restriction of murine norovirus infection in vivo

1. Mridula Annaswamy Srinivas
2. Linley R. Pierce
3. Mikayla C. Olson
4. Shelly J. Roberston
5. Gail L. Sturdevant
6. Sonja M. Best
7. Robert C. Orchard

Reviewed by Arcadia Science

Salmonella-induced SIRT1 and SIRT3 are crucial for maintaining the metabolic switch in bacteria and host for successful pathogenesis

1. Dipasree Hajra
2. Raju S Rajmani
3. Ayushi Devendrasingh Chaudhary
4. Shashi Kumar Gupta
5. Dipshikha Chakravortty

• Curated by eLife

  eLife Assessment

  This auhors present findings on the role of the sirtuins SIRT1 and SIRT3 during Salmonella Typhimurium infection. This valuable study increases our understanding of the mechanisms used by this pathogen to interact with its host and may have implications for other intracellular pathogens. The reviewers disagreed on the strength of the evidence to support the claims. Although one reviewer found the strength of the evidence convincing, the other found that it was incomplete, and that the main claims are only partially supported, as can be seen from the public reviews.

Reviewed by eLife

PfMORC protein regulates chromatin accessibility and transcriptional repression in the human malaria parasite, Plasmodium falciparum

1. Zeinab M Chahine
2. Mohit Gupta
3. Todd Lenz
4. Thomas Hollin
5. Steven Abel
6. Charles Banks
7. Anita Saraf
8. Jacques Prudhomme
9. Suhani Bhanvadia
10. Laurence A Florens
11. Karine G Le Roch

• Curated by eLife

  eLife Assessment

  This important study examines the role of Microrchidia (MORC) proteins in the human malaria parasite Plasmodium falciparum. Solid experimental results, including genome editing and chromatin profiling methods (ChIP-seq and Hi-C), provide a comprehensive picture of the critical role MORC plays in shaping parasite chromatin. Depletion of MORC results in a lethal collapse of heterochromatin and parasite death, nominating the factor as a new target of antimalarial therapies.

Reviewed by eLife

Bacillus velezensis HBXN2020 alleviates Salmonella Typhimurium infection in mice by improving intestinal barrier integrity and reducing inflammation

1. Linkang Wang
2. Haiyan Wang
3. Xinxin Li
4. Mengyuan Zhu
5. Dongyang Gao
6. Dayue Hu
7. Zhixuan Xiong
8. Xiangmin Li
9. Ping Qian

• Curated by eLife

  eLife Assessment

  In this useful study, Wang and colleagues investigate the potential probiotic effects of Bacillus velezensis in a murine model. They provide convincing evidence that B. velezensis limits the growth of Salmonella typhimurium in lab culture and in mice, together with beneficial effects on the microbiota. The overall presentation of the manuscript has improved and the work will be of interest to infectious disease researchers.

Reviewed by eLife

Mixed alkyl/aryl phosphonates identify metabolic serine hydrolases as antimalarial targets

1. John M. Bennett
2. Sunil K. Narwal
3. Stephanie Kabeche
4. Daniel Abegg
5. Vandana Thathy
6. Fiona Hackett
7. Tomas Yeo
8. Veronica L. Li
9. Ryan Muir
10. Franco Faucher
11. Scott Lovell
12. Michael J. Blackman
13. Alexander Adibekian
14. Ellen Yeh
15. David A. Fidock
16. Matthew Bogyo

Reviewed by preLights

Reduced gravity and muon radiation affect Caenorhabditis elegans life history traits and viral infection

1. Ana Villena-Giménez
2. Victoria G. Castiglioni
3. Juan C. Muñoz-Sánchez
4. Esmeralda G. Legarda
5. Rubén González
6. Santiago F. Elena

Reviewed by Arcadia Science

Host-derived Lactobacillus plantarum alleviates hyperuricemia by improving gut microbial community and hydrolase-mediated degradation of purine nucleosides

1. Yang Fu
2. Xiao-Dan Luo
3. Jin-Ze Li
4. Qian-Yuan Mo
5. Xue Wang
6. Yue Zhao
7. You-Ming Zhang
8. Hao-Tong Luo
9. Dai-Yang Xia
10. Wei-Qing Ma
11. Jian-Ying Chen
12. Li-Hau Wang
13. Qiu-Yi Deng
14. Lukuyu Ben
15. Muhammad Kashif Saleemi
16. Xian-Zhi Jiang
17. Juan Chen
18. Kai Miao
19. Zhen-Ping Lin
20. Peng Zhang
21. Hui Ye
22. Qing-Yun Cao
23. Yong-Wen Zhu
24. Lin Yang
25. Qiang Tu
26. Wence Wang

• Curated by eLife

  eLife Assessment

  The landmark significance of this manuscript is based on the mechanistic description of purine metabolism by Lactobacillus plantarum, which helps to alleviate hyperuricemia, which is a phenotype that underlies multiple disease symptoms. The evidence provided for L. plantarum's involvement in reducing hyperuricemia was exceptional, combining microbiomics, whole genomics, in vitro bacterial culture, gene knock-outs, and metabolomics. Collectively, the study shows a clear link between the gut microbiota and hyperuricemia, providing a pathway for modification to help alleviate this condition.

Reviewed by eLife

Intestinal microbiome dysbiosis increases Mycobacteria pulmonary colonization in mice by regulating the Nos2-associated pathways

1. MeiQing Han
2. Xia Wang
3. Lin Su
4. Shiqi Pan
5. Ningning Liu
6. Duan Li
7. Liang Liu
8. JunWei Cui
9. Huajie Zhao
10. Fan Yang

• Curated by eLife

  eLife Assessment

  This important study shows the effect of gut dysbiosis on the colonization of mycobacteria in the lung. The data with comprehensive analysis of gene expression profiles in the lung with dysbiotic mice is compelling and goes beyond the current state of the art. However, the mechanistic insight, where the lung epithelial cell line was used, and the experiments with Mtb infection are currently incomplete.

Reviewed by eLife

A conserved cell-pole determinant organizes proper polar flagellum formation

1. Erick E Arroyo-Pérez
2. John C Hook
3. Alejandra Alvarado
4. Stephan Wimmi
5. Timo Glatter
6. Kai Thormann
7. Simon Ringgaard

• Curated by eLife

  eLife assessment

  This important study describes the discovery of a mechanism by which multiple species of bacteria synthesize and localize polar flagella via a novel protein, FipA, which interacts with FlhF. The authors use appropriate methodological approaches (biochemistry, molecular microbiology, quantitative microscopy, and bacterial genetics) to obtain and present convincing results and interpretations. This work will particularly interest those studying bacterial motility and bacterial cell biologists.

Reviewed by eLife