Microbiology

Misic, a general deep learning-based method for the high-throughput cell segmentation of complex bacterial communities

1. Swapnesh Panigrahi
2. Dorothée Murat
3. Antoine Le Gall
4. Eugénie Martineau
5. Kelly Goldlust
6. Jean-Bernard Fiche
7. Sara Rombouts
8. Marcelo Nöllmann
9. Leon Espinosa
10. Tâm Mignot

• Curated by eLife

  Evaluation Summary:

  In this work, Panigrahi et. al. develop a powerful deep-learning-based cell segmentation platform (MiSiC) capable of accurately segmenting brightfield, fluorescence, and phase contrast images of bacteria cells densely packed within both homogenous and heterogeneous cell populations. This algorithm, if further optimized and disseminated to the community, will have a large impact in microbial studies in that it will allow for automated analyses of essential every aspect of bacterial cell biology, including cell morphology, cell cycle, cell-cell communications, protein localization dynamics and a variety of cellular processes using time-lapse imaging.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Uncovering novel endolysins against methicillin-resistant Staphylococcus aureus using microbial single-cell genome sequencing

1. Takuya Yoda
2. Ayumi Matsuhashi
3. Ai Matsushita
4. Shohei Shibagaki
5. Yukie Sasakura
6. Kazuteru Aoki
7. Masahito Hosokawa
8. Soichiro Tsuda

Reviewed by Arcadia Science

Parallel evolution between genomic segments of seasonal human influenza viruses reveals RNA-RNA relationships

1. Jennifer E Jones
2. Valerie Le Sage
3. Gabriella H Padovani
4. Michael Calderon
5. Erik S Wright
6. Seema S Lakdawala

Reviewed by eLife

Single mosquito metatranscriptomics identifies vectors, emerging pathogens and reservoirs in one assay

1. Joshua Batson
2. Gytis Dudas
3. Eric Haas-Stapleton
4. Amy L Kistler
5. Lucy M Li
6. Phoenix Logan
7. Kalani Ratnasiri
8. Hanna Retallack

Reviewed by Review Commons

Colistin kills bacteria by targeting lipopolysaccharide in the cytoplasmic membrane

1. Akshay Sabnis
2. Katheryn LH Hagart
3. Anna Klöckner
4. Michele Becce
5. Lindsay E Evans
6. R Christopher D Furniss
7. Despoina AI Mavridou
8. Ronan Murphy
9. Molly M Stevens
10. Jane C Davies
11. Gérald J Larrouy-Maumus
12. Thomas B Clarke
13. Andrew M Edwards

Reviewed by eLife

Sensitizing Staphylococcus aureus to antibacterial agents by decoding and blocking the lipid flippase MprF

1. Christoph J Slavetinsky
2. Janna N Hauser
3. Cordula Gekeler
4. Jessica Slavetinsky
5. André Geyer
6. Alexandra Kraus
7. Doris Heilingbrunner
8. Samuel Wagner
9. Michael Tesar
10. Bernhard Krismer
11. Sebastian Kuhn
12. Christoph M Ernst
13. Andreas Peschel

• Curated by eLife

  Evaluation Summary:

  This study is of interest to readers in the field of Microbiology and the control of microbial infectious diseases. The authors address the challenge of antibiotic resistant bacteria with an innovative anti-virulence approach using monoclonal antibodies against a Staphylococcus aureus lipid flippase involved in tolerance to cationic peptides. The work indicates that this approach could re-sensitize antibiotic resistant S. aureus and diminish the severity of infections.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Unexpected plasticity in the life cycle of Trypanosoma brucei

1. Sarah Schuster
2. Jaime Lisack
3. Ines Subota
4. Henriette Zimmermann
5. Christian Reuter
6. Tobias Mueller
7. Brooke Morriswood
8. Markus Engstler

• Curated by eLife

  Evaluation Summary:

  This paper describes the transmission of Trypanosoma brucei by the Tsetse vector. As part of these studies, the authors discovered that (i) a single parasite is sufficient for transmission and (ii) two stages of the Trypanosoma brucei life cycle (slender and stumpy forms) can be transmitted by the Tsetse vector - although the stumpy form developed more rapidly into proliferative parasites in the Tsetse midgut. The results are unexpected because it was previously thought that only stumpy forms were important for transmission.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife, preLights

Susceptibility of White-Tailed Deer (Odocoileus virginianus) to SARS-CoV-2

1. Mitchell V. Palmer
2. Mathias Martins
3. Shollie Falkenberg
4. Alexandra Buckley
5. Leonardo C. Caserta
6. Patrick K. Mitchell
7. Eric D. Cassmann
8. Alicia Rollins
9. Nancy C. Zylich
10. Randall W. Renshaw
11. Cassandra Guarino
12. Bettina Wagner
13. Kelly Lager
14. Diego G. Diel

Reviewed by ScreenIT, NCRC

A human coronavirus evolves antigenically to escape antibody immunity

1. Rachel T. Eguia
2. Katharine H. D. Crawford
3. Terry Stevens-Ayers
4. Laurel Kelnhofer-Millevolte
5. Alexander L. Greninger
6. Janet A. Englund
7. Michael J. Boeckh
8. Jesse D. Bloom

Reviewed by PREreview, ScreenIT, Review Commons

A novel SARS-CoV-2 related coronavirus in bats from Cambodia

1. Vibol Hul
2. Deborah Delaune
3. Erik A Karlsson
4. Alexandre Hassanin
5. Putita Ou Tey
6. Artem Baidaliuk
7. Fabiana Gámbaro
8. Vuong Tan Tu
9. Lucy Keatts
10. Jonna Mazet
11. Christine Johnson
12. Philippe Buchy
13. Philippe Dussart
14. Tracey Goldstein
15. Etienne Simon-Lorière
16. Veasna Duong

Reviewed by ScreenIT, NCRC