Microbiology

Alternative cell entry mechanisms for SARS-CoV-2 and multiple animal viruses

1. Ravi Ojha
2. Anmin Jiang
3. Elina Mäntylä
4. Naphak Modhira
5. Robert Witte
6. Arnaud Gaudin
7. Lisa De Zanetti
8. Rachel Gormal
9. Maija Vihinen-Ranta
10. Jason Mercer
11. Maarit Suomalainen
12. Urs F. Greber
13. Yohei Yamauchi
14. Pierre Yves-Lozach
15. Ari Helenius
16. Olli Vapalahti
17. Paul Young
18. Daniel Watterson
19. Frédéric A. Meunier
20. Merja Joensuu
21. Giuseppe Balistreri

Reviewed by Review Commons

Eukaryotic CD-NTase, STING, and viperin proteins evolved via domain shuffling, horizontal transfer, and ancient inheritance from prokaryotes

1. Edward M. Culbertson
2. Tera C. Levin

Reviewed by Review Commons

Interferon induced circRNAs escape herpesvirus host shutoff and suppress lytic infection

1. Sarah E Dremel
2. Takanobu Tagawa
3. Vishal N Koparde
4. Carmen Hernandez-Perez
5. Jesse H Arbuckle
6. Thomas M Kristie
7. Laurie T Krug
8. Joseph M Ziegelbauer

Reviewed by Review Commons

Complete Protection from SARS-CoV-2 Lung Infection in Mice Through Combined Intranasal Delivery of PIKfyve Kinase and TMPRSS2 Protease Inhibitors

1. Ravi Kant
2. Lauri Kareinen
3. Ravi Ojha
4. Tomas Strandin
5. Saber Hassan Saber
6. Angelina Lesnikova
7. Suvi Kuivanen
8. Tarja Sirnonen
9. Merja Joensuu
10. Olli Vapalahti
11. Tom Kirchhausen
12. Anja Kipar
13. Giuseppe Balistreri

Reviewed by Review Commons

PI(3,4,5)P3 allosteric regulation of repressor activator protein 1 controls antigenic variation in trypanosomes

1. Abdoulie O Touray
2. Rishi Rajesh
3. Tony Isebe
4. Tamara Sternlieb
5. Mira Loock
6. Oksana Kutova
7. Igor Cestari

Reviewed by eLife

Macrophage- and CD4 ⁺ T cell-derived SIV differ in glycosylation, infectivity and neutralization sensitivity

1. Christina B. Karsten
2. Falk F.R. Buettner
3. Samanta Cajic
4. Inga Nehlmeier
5. Berit Roshani
6. Antonina Klippert
7. Ulrike Sauermann
8. Nicole Stolte-Leeb
9. Udo Reichl
10. Rita Gerardy-Schahn
11. Erdmann Rapp
12. Christiane Stahl-Hennig
13. Stefan Pöhlmann

Reviewed by Review Commons

A remarkable genetic shift in a transmitted/founder virus broadens antibody responses against HIV-1

1. Swati Jain
2. Gherman Uritskiy
3. Marthandan Mahalingam
4. Himanshu Batra
5. Subhash Chand
6. Hung V Trinh
7. Charles Beck
8. Woong-Hee Shin
9. Wadad Alsalmi
10. Gustavo Kijak
11. Leigh A Eller
12. Jerome Kim
13. Daisuke Kihara
14. Sodsai Tovanabutra
15. Guido Ferrari
16. Merlin L Robb
17. Mangala Rao
18. Venigalla B Rao

• Curated by eLife

  eLife assessment

  This study provides a detailed evaluation of how HIV evades nascent immune pressure from people living with HIV followed nearly immediately after infection. There is convincing evidence that H173 mutations in the V2 loop was a key determinant of selection pressure and escape. These data are congruent with protection in the RV144 clinical trial, the only trial that showed protection from infection. Overall, this study is an important contribution to the field.

Reviewed by eLife

Un1Cas12f1 and Cas9 gene drive in HSV1: viruses that ‘infect’ viruses

1. Qiaorui Yao
2. Zhuangjie Lin
3. Keyuan Lai
4. Xianying Zeng
5. Guanxiong Lei
6. Tongwen Zhang
7. Hongsheng Dai

• Curated by eLife

  eLife assessment

  This valuable paper by Yao, Dai, and colleagues describes a viral gene drive against herpes simplex virus 1 in cell culture. The authors provided solid evidence that an engineered gene drive sequence, expressing either spCas9 or Un1Cas12f1 nuclease, could spread efficiently in the population of wild-type viruses and induce fewer drive-resistant mutations than spCas9. Limitations include a mechanistically inaccurate title, several methodologic flaws, and a paucity of descriptions of possible therapeutic applications.

Reviewed by eLife

Structural insights into peptidoglycan hydrolysis by the FtsEX system in Escherichia coli during cell division

1. Jianwei Li
2. Yutong He
3. Xin Xu
4. Martin Alcorlo
5. Jian Shi
6. David I. Roper
7. Juan A. Hermoso
8. Lok-To Sham
9. Min Luo

• Curated by eLife

  eLife assessment

  This is a useful study that provides solid, yet confirmatory findings about the complex (FtsEX) that controls peptidoglycan remodeling during bacterial cell division. The authors capitalize on the finding that ATP binding stabilizes the FtsEX complex allowing structural characterization for this system. A model is then developed using biochemical approaches to explain ATP regulation. The resulting model would be strengthened were the authors to incorporate their structural findings as well as previously published work.

Reviewed by eLife

Virion morphology and on-virus spike protein structures of diverse SARS-CoV-2 variants

1. Zunlong Ke
2. Thomas P. Peacock
3. Jonathan C. Brown
4. Carol M. Sheppard
5. Tristan I. Croll
6. Abhay Kotecha
7. Daniel H. Goldhill
8. Wendy S. Barclay
9. John A.G. Briggs

Reviewed by PREreview