Microbiology

SIRT2 protects against Japanese encephalitis virus infection in mice

1. Perumal Arumugam Desingu
2. Lavanya Dindi
3. Krishnega Murugasamy
4. Ankit Kumar Tamta
5. Venketsubbu Ramasubbu
6. Sukanya Raghu
7. Amarjeet Shrama
8. Raju S Rajmani
9. Nagalingam R Sundaresan

• Curated by eLife

  eLife Assessment

  This important study explores the role of SIRT2 in regulating Japanese encephalitis virus replication and disease progression in rodent models. The findings presented are novel as sirtuins are known for their roles in aging, metabolism, and cell survival, but have not been studied in the context of viral infections until recently. The evidence supporting the claims is solid, although additional experiments to further characterize the clinical outcomes and directly test the link between acetylated NF-kB and SIRT2 expression would have strengthened the study. The work will be of interest to biologists studying viruses, sirtuins, and inflammation.

Reviewed by eLife

Multi-omics single-cell analysis reveals key regulators of HIV-1 persistence and aberrant host immune responses in early infection

1. Dayeon Lee
2. Sin Young Choi
3. So-I Shin
4. Hyunsu An
5. Byeong-Sun Choi
6. Jihwan Park

• Curated by eLife

  eLife Assessment

  This study presents important findings that enhance our understanding of immune cell interactions in the context of chronic HIV-1 infection. The evidence supporting the conclusions is convincing. The authors have employed appropriate and validated methodologies, including detailed data reprocessing and batch correction to account for inter-donor variability. The inclusion of supplementary figures and analyses, such as cell communication inference, further substantiates the robustness of the findings. Overall, this work contributes to our understanding of HIV-1 immune evasion and highlights potential therapeutic targets for reservoir eradication.

Reviewed by eLife

Unraveling CRP/cAMP-mediated metabolic regulation in Escherichia coli persister cells

1. Han G Ngo
2. Sayed Golam Mohiuddin
3. Aina Ananda
4. Mehmet Orman

• Curated by eLife

  eLife Assessment

  The study reports an important finding on the role of the global metabolic regulator Crp/cAMP in the formation of antibiotic persister Escherichia coli. The evidence supporting the claims is solid including metabolomic analysis and characterization of many mutant strains.

Reviewed by eLife

Heterogeneous efflux pump expression underpins phenotypic resistance to antimicrobial peptides

1. Ka Kiu Lee
2. Urszula Łapińska
3. Giulia Tolle
4. Maureen Micaletto
5. Bing Zhang
6. Wanida Phetsang
7. Anthony D Verderosa
8. Brandon M Invergo
9. Joseph Westley
10. Attila Bebes
11. Raif Yuecel
12. Paul A O'Neill
13. Audrey Farbos
14. Aaron R Jeffries
15. Stineke van Houte
16. Pierluigi Caboni
17. Mark AT Blaskovich
18. Benjamin E Housden
19. Krasimira Tsaneva-Atanasova
20. Stefano Pagliara

• Curated by eLife

  eLife Assessment

  This important study by Lee et al. explores the heterogeneous response of non-growing bacteria to the antimicrobial peptide (AMP) tachyplesin. The authors identify a subpopulation of cells that evade lethal damage by limiting the intracellular accumulation of a fluorescently labeled tachyplesin analog. The study provides compelling evidence that reduced drug accumulation underlies the decreased susceptibility of this subpopulation to the AMP. The molecular basis of this phenotype is well supported by the data.

Reviewed by eLife

Deletion of the moeA gene in Flavobacterium IR1 drives structural color shift from green to blue and alters polysaccharide metabolism

1. Álvaro Escobar Doncel
2. Constantinos Patinios
3. Alexandre Campos
4. Maria Beatriz Walter Costa
5. Maria V Turkina
6. Maria Murace
7. Raymond HJ Staals
8. Silvia Vignolini
9. Bas E Dutilh
10. Colin J Ingham

• Curated by eLife

  eLife Assessment

  This manuscript presents important findings on how structural color can be manipulated through a specific single-gene mutation in a motile bacterium. Compelling data provide a promising model to identify genes and molecular mechanisms supporting this widespread optical phenomenon. This work will be of interest to biophysicists and microbiologists working on structural colors and Flavobacterium.

Reviewed by eLife

Flexibility and modulation of translation initiation in enterovirus genomes

1. Rhian L. O'Connor
2. Georgia M. Cook
3. Jacqueline Hankinson
4. Ksenia Fominykh
5. Samantha H. Cheng
6. Daniel A. Nash
7. Aurélie Cenier
8. Komal M. Nayak
9. Stephen C. Graham
10. Janet E. Deane
11. Matthias Zilbauer
12. Andrew E. Firth
13. Valeria Lulla

Reviewed by Review Commons

Azithromycin alters the microbiome composition, function and resistome in women with Chlamydia trachomatis infections

1. Sankhya Bommana
2. Olusola Olagoke
3. Yi-Juan Hu
4. Ruohong Wang
5. Mike Kama
6. Morgan Dehdashti
7. Reshma Kodimerla
8. Timothy D. Read
9. Deborah Dean

Reviewed by Rapid Reviews Infectious Diseases

Function and firing of the Streptomyces coelicolor contractile injection system requires the membrane protein CisA

1. Bastien Casu
2. Joseph W Sallmen
3. Peter E Haas
4. Govind Chandra
5. Pavel Afanasyev
6. Jingwei Xu
7. Martin Pilhofer
8. Susan Schlimpert

• Curated by eLife

  eLife Assessment

  This valuable study provides insights into the structure and function of bacterial contractile injection systems that are present in the cytoplasm of many Streptomyces strains. A convincing high-resolution model of the structure of extended forms of the cytoplasmic contractile injection system assembly from Streptomyces coelicolor is presented, with some investigation of the membrane protein CisA in attachment of the extended assembly to the inner face of the cytoplasmic membrane and the firing of the system. The work expands the current understanding of these diverse bacterial nanomachines.

Reviewed by eLife

The microbiome interacts with the circadian clock and dietary composition to regulate metabolite cycling in the Drosophila gut

1. Yueliang Zhang
2. Sara B Noya
3. Yongjun Li
4. Jichao Fang
5. Amita Sehgal

• Curated by eLife

  eLife Assessment

  This study presents valuable findings about daily rhythm changes of the Drosophila melanogaster adult gut metabolome, which is shown to be dependent on the circadian clock genotype, dietary regime and composition, and gut microbiota. The phenomena observed are supported by convincing experimental evidence. The general descriptive approach limits the power of the proposed conclusions. The work will be of interest to a broad range of physiology specialists

Reviewed by eLife

Common biological features of Mycobacterium tuberculosis MmpL3 inhibitors

1. Lauren Ames
2. Renee Allen
3. Helena I. Boshoff
4. Laura Cleghorn
5. Curtis Engelhart
6. Dirk Schnappinger
7. Tanya Parish

Reviewed by Rapid Reviews Infectious Diseases