Microbiology

Mapping the ultrastructural topology of the corynebacterial cell surface

1. Buse Isbilir
2. Anna Yeates
3. Vikram Alva
4. Tanmay A. M. Bharat

Reviewed by Review Commons

Inflammasomes primarily restrict cytosolic Salmonella replication within human macrophages

1. Marisa S Egan
2. Emily A O'Rourke
3. Shrawan Kumar Mageswaran
4. Biao Zuo
5. Inna Martynyuk
6. Tabitha Demissie
7. Emma N Hunter
8. Antonia R Bass
9. Yi-Wei Chang
10. Igor E Brodsky
11. Sunny Shin

• Curated by eLife

  eLife Assessment

  This paper provides fundamental insights into the control of Salmonella within human macrophages, with convincing evidence that Salmonella can replicate in the macrophage cytosol in the absence of inflammasome signaling. This paper, which improves our understanding of how the immune system fights bacterial infections, will be of broad interest to cell biologists, immunologists and microbiologists.

Reviewed by eLife

Combination of mitomycin C and low-dose metronidazole synergistically against Clostridioides difficile infection and recurrence prevention

1. Jun-Jia Gong
2. I-Hsiu Huang
3. Yuan-Pin Hung
4. Yi-Wei Chen
5. Yun-Chien Lin
6. Jenn-Wei Chen

Reviewed by Rapid Reviews Infectious Diseases

Birth of new protein folds and functions in the virome

1. Jason Nomburg
2. Nathan Price
3. Jennifer A. Doudna

Reviewed by Arcadia Science

Aminoglycoside tolerance in Vibrio cholerae engages translational reprogramming associated with queuosine tRNA modification

1. Louna Fruchard
2. Anamaria Babosan
3. Andre Carvalho
4. Manon Lang
5. Blaise Li
6. Magalie Duchateau
7. Quentin Giai Gianetto
8. Mariette Matondo
9. Frederic Bonhomme
10. Isabelle Hatin
11. Hugo Arbes
12. Céline Fabret
13. Enora Corler
14. Guillaume Sanchez
15. Virginie Marchand
16. Yuri Motorin
17. Olivier Namy
18. Valérie de Crécy-Lagard
19. Didier Mazel
20. Zeynep Baharoglu

• Curated by eLife

  eLife Assessment

  This study investigates the role of queuosine (Q) tRNA modification in aminoglycoside tolerance in Vibrio cholerae and presents convincing evidence to conclude that Q is essential for the efficient translation of TAT codons, although this depends on the context. The absence of Q reduces aminoglycoside tolerance potentially by reprogramming the translation of an oxidative stress response gene, rxsA. Overall, the findings point to an important mechanism whereby changes in Q modification levels control the decoding of mRNAs enriched in TAT codons under antibiotic stress.

Reviewed by eLife

Integrating microscopy and transcriptomics from individual uncultured eukaryotic plankton

1. Catherine Gatt
2. Yike Xie
3. Kanu Wahi
4. Emma MV Johansson
5. Fabio Zanini

• Curated by eLife

  eLife Assessment

  This valuable research contributes to our understanding of marine plankton diversity and gene expression by employing robust methodologies for sample collection and analysis. However, it lacks a comprehensive comparison with existing single-cell transcriptomics techniques in microbial ecology, and some terminology requires clarification for consistency with field standards. The downstream data analysis therefore provides only incomplete support for the claims made by the authors.

Reviewed by eLife, Arcadia Science

Cryo-EM structure and polar assembly of the PS2 S-layer of Corynebacterium glutamicum

1. Adrià Sogues
2. Mike Sleutel
3. Julienne Petit
4. Daniela Megrian
5. Nicolas Bayan
6. Anne Marie Wehenkel
7. Han Remaut

Reviewed by Review Commons

Oxydifficidin, a potent Neisseria gonorrhoeae antibiotic due to DedA-assisted uptake and ribosomal protein RplL sensitivity

1. Jingbo Kan
2. Adrian Morales-Amador
3. Yozen Hernandez
4. Melinda A Ternei
5. Christophe Lemetre
6. Logan W Maclntyre
7. Nicolas Biais
8. Sean F Brady

• Curated by eLife

  eLife Assessment

  Kan et al. report the discovery of a Bacillus amyloliquifaciens strain that kills Nerisseria gonorrhoeae via oxydifficidin which targets ribosomal proteins. Resistance occurred via mutation in the DedA flippase to influence oxydifficidin uptake. The overall mechanism of action is well described making this an important study with implications for combating clinical antibiotic resistance. The evidence presented is convincing due to rigour employed in the methodological approach. The authors should consider performing a more comprehensive genetic analyses of DedA and RpIL in this clinically relevant strain. This work will be of broad interest to microbiologists and synthetic biologists.

Reviewed by eLife

The relationship between gut and nasopharyngeal microbiome composition can predict the severity of COVID-19

1. Benita Martin-Castaño
2. Patricia Diez-Echave
3. Jorge García-García
4. Laura Hidalgo-García
5. Antonio Jesús Ruiz-Malagon
6. José Alberto Molina-Tijeras
7. María Jesús Rodríguez-Sojo
8. Anaïs Redruello-Romero
9. Margarita Martínez-Zaldívar
10. Emilio Mota
11. Fernando Cobo
12. Xando Díaz-Villamarin
13. Marta Alvarez-Estevez
14. Federico García
15. Concepción Morales-García
16. Silvia Merlos
17. Paula Garcia-Flores
18. Manuel Colmenero-Ruiz
19. José Hernández-Quero
20. Maria Nuñez
21. Maria Elena Rodriguez-Cabezas
22. Angel Carazo
23. Javier Martin
24. Rocio Moron
25. Alba Rodríguez Nogales
26. Julio Galvez

• Curated by eLife

  eLife Assessment

  This potentially valuable work characterizes the changes in the microbial composition of the nasal and fecal microbiomes in COVID-19 patients based on disease severity. This study enhances the understanding of COVID-19 severity predictors by identifying changes in bacterial species abundance in nasopharyngeal and fecal samples as a biomarker for predicting disease severity. The methods and statistics used appear to be solid and in line with the standards of the field.

Reviewed by eLife

Magnesium modulates phospholipid metabolism to promote bacterial phenotypic resistance to antibiotics

1. Hui Li
2. Jun Yang
3. Su-fang Kuang
4. Huan-zhe Fu
5. Hui-yin Lin
6. Bo Peng

• Curated by eLife

  eLife Assessment

  The study explored the influence of magnesium on phenotypic antibiotic resistance in two strains of Vibrios: V. alginolyticus ATCC33787 and V. parahaemolyticus VP01. This research is fundamental for revealing the phenotypic antibiotic resistance mechanism utilized by the specified model bacteria in elevated levels of magnesium. The study produced convincing evidence indicating that in high concentrations of magnesium, the efficacy of selected antibiotics was diminished due to decreased biosynthesis of unsaturated fatty acids and PE, along with an increase in the biosynthesis of PG.

Reviewed by eLife