Microbiology

A bacterial regulatory uORF senses multiple classes of ribosome-targeting antibiotics

1. Gabriele Baniulyte
2. Joseph T Wade

• Curated by eLife

  eLife Assessment

  In this important study, Baniulyte and Wade provide convincing evidence that translation of a short ORF denoted toiL positioned upstream of the topAI-yjhQP operon is responsive to different ribosome-targeting antibiotics, consequently controlling translation of the TopAI toxin as well as Rho-dependent transcription termination. Strengths of the study include combining a genetic screen to identify 23S rRNA mutations that affect topA1 expression and a creative approach to map the different locations of ribosome stalling within toiL induced by different antibiotics, with ribosome profiling and RNA structure probing by SHAPE to examine consequences of different antibiotics on toiL-mediated regulation. The work leaves unanswered how bacteria benefit by activating expression of the genes using the proposed strategy and the mechanism underlying ToiL's sensing of structurally distinct antibiotics.

Reviewed by eLife

Vibrio cholerae lineage and pangenome diversity varies geographically across Bangladesh over one year

1. Chuhan Qin
2. Patrick Lypaczewski
3. Md. Abu Sayeed
4. Aline C. Cuénod
5. Lindsey Brinkley
6. Ashton Creasy-Marrazzo
7. Emilee T. Cato
8. Kamrul Islam
9. Md. Imam Ul Khabir
10. Md. Taufiqur R. Bhuiyan
11. Yasmin Begum
12. Firdausi Qadri
13. Ashraful I. Khan
14. Eric J. Nelson
15. B. Jesse Shapiro

Reviewed by Rapid Reviews Infectious Diseases

The emergence of new lineages of the Mpox virus could affect the 2022 outbreak

1. Mayla Abrahim
2. Alexandro Guterres
3. Patrícia Cristina da Costa Neves
4. Ana Paula Dinis Ano Bom

Reviewed by Rapid Reviews Infectious Diseases

Efficacy and mechanism of action of cipargamin as an antibabesial drug candidate

1. Hang Li
2. Shengwei Ji
3. Nanang R Ariefta
4. Eloiza May S Galon
5. Shimaa AES El-Sayed
6. Thom Do
7. Lijun Jia
8. Miako Sakaguchi
9. Masahito Asada
10. Yoshifumi Nishikawa
11. Xin Qin
12. Mingming Liu
13. Xuenan Xuan

• Curated by eLife

  eLife Assessment

  This study presents valuable findings with practical and theoretical implications for drug discovery, particularly in the context of repurposing cipargamin CIP for the treatment of Babesia spp. The evidence is solid with the methods, data, and analyses broadly supporting the claims. The paper will be of great interest to scientists in drug discovery, computational biology, and microbiology.

Reviewed by eLife

Primosomal protein PriC rescues replication initiation stress by bypassing the DnaA-DnaB interaction step for DnaB helicase loading at oriC

1. Ryusei Yoshida
2. Kazuma Korogi
3. Qinfei Wu
4. Shogo Ozaki
5. Tsutomu Katayama

• Curated by eLife

  eLife Assessment

  This manuscript reports findings of fundamental significance on how bacteria might load helicase for DNA replication when normal DnaA-based loading pathway is defective. It provides convincing genetic and biochemical evidence that helicase loading at the E. coli oriC is not (as previously assumed) exclusively performed by the DnaA initiator protein but can also be executed by PriC (whether this occurs specifically at oriC has not been addressed in vivo). This is a significant step forward in our understanding of bacterial replication initiation.

Reviewed by eLife

Electromagnetic waves destabilize the SARS-CoV-2 Spike protein and reduce SARS-CoV-2 Virus-Like Particle (SC2-VLP) infectivity

1. Skyler Granatir
2. Francisco M. Acosta
3. Christina Pantoja
4. Johann Tailor
5. Angus Fuori
6. Bill Dower
7. Henry Marr
8. Peter W. Ramirez

Reviewed by PREreview

Metagenomes and Metagenome-Assembled Genomes from Tidal Lagoons at a New York City Waterfront Park

1. Sally Kong
2. Eliana Abrams
3. Yehuda Binik
4. Christina Cappelli
5. Mathew Chu
6. Taiyo Cornett
7. Isayah Culbertson
8. Epifania Garcia
9. Jada Henry
10. Kristy Lam
11. DB Lampman
12. Grace Morenko
13. Illusion Rivera
14. Tanasia Swift
15. Isabella Torres
16. Rayven Velez
17. Elliot Waxman
18. Serena Wessely
19. Anthony Yuen
20. Casey K. Lardner
21. JL Weissman

Reviewed by Arcadia Science

A Blueprint for Broadly Effective Bacteriophage Therapy Against Bacterial Infections

1. Minyoung Kevin Kim
2. Qingquan Chen
3. Arne Echterhof
4. Robert C. McBride
5. Nina Pennetzdorfer
6. Niaz Banaei
7. Elizabeth B. Burgener
8. Carlos E. Milla
9. Paul L. Bollyky

Reviewed by Rapid Reviews Infectious Diseases, Arcadia Science, PREreview

Modulation of host signalling pathways reveal a major role for Wnt signalling in the maturation of Plasmodium falciparum liver schizonts

1. Abhishek Kanyal
2. Geert-Jan van Gemert
3. Haoyu Wu
4. Alex van der Starre
5. Johannes HW de Wilt
6. Teun Bousema
7. Robert W. Sauerwein
8. Richard Bartfai
9. Annie SP Yang

Reviewed by Rapid Reviews Infectious Diseases

Mosquito immune cells enhance dengue and Zika virus dissemination in Aedes aegypti

1. David R. Hall
2. Rebecca M. Johnson
3. Hyeogsun Kwon
4. Zannatul Ferdous
5. S. Viridiana Laredo-Tiscareño
6. Bradley J. Blitvich
7. Doug E. Brackney
8. Ryan C. Smith

Reviewed by Rapid Reviews Infectious Diseases