Microbiology

Physiological function of Flo11p domains and the particular role of amyloid core sequences of this adhesin in Saccharomyces cerevisiae

1. Clara Bouyx
2. Marion Schiavone
3. Marie-Ange Teste
4. Etienne Dague
5. Nathalie Sieczkowski
6. Anne Julien
7. Jean Marie Francois

• Curated by eLife

  Evaluation Summary:

  This work serves as an independent confirmation and expansion of previous work, suggesting that certain domains in fungal adhesins can form amyloid-like structures that are essential for cell-cell aggregation, but less so for cell-surface adhesion or invasive growth. Specifically, the expression of different adhesin constructs offers insight into the functional role of the different adhesin domains. Together, the results provide further insight into the molecular mechanisms underlying fungal adhesion, which does only further our understanding of basic fungal physiology, but also offers insight into fungal pathogenesis.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

An essential, kinetoplastid-specific GDP-Fuc: β-D-Gal α-1,2-fucosyltransferase is located in the mitochondrion of Trypanosoma brucei

1. Giulia Bandini
2. Sebastian Damerow
3. Maria Lucia Sempaio Guther
4. Hongjie Guo
5. Angela Mehlert
6. Jose Carlos Paredes Franco
7. Stephen Beverley
8. Michael AJ Ferguson

• Curated by eLife

  Evaluation Summary:

  This study demonstrates for the first time that the single annotated fucosyltransferase (TbFUT1) in the parasitic protist, Trypanosoma brucei is specifically targeted to the mitochondrion, rather than in the secretory pathway or cytoplasm, as in other eukaryotes. TbFUT1 utilizes glycan substrates in vitro and conditional down-regulation of TbFUT1 expression causes a severe growth defect in the two major developmental stages of these pathogens, indicating that it is essential for pathogenesis and a potential drug target.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Protein-based RBD-C-tag COVID-19 Vaccination Candidate Elicits Protection Activity against SARS-COV-2 Variant Infection

1. Jaganathan Subramani
2. Namir Shaabani
3. Dhwani Shetty
4. Haiwa Wu
5. Sunkuk Kwon
6. Wenzhao Li
7. Chanyu Yue
8. Christoph Lahtz
9. Adela Ramirez-Torres
10. Heyue Zhou
11. Yanliang Zhang
12. Robert Allen
13. Bill Farley
14. Mark Emalfarb
15. Ronen Tchelet
16. Soloheimo Markku
17. Vitikainen Marika
18. Marilyn Wiebe
19. Anne Huuskonen
20. Hannah Ben-artzi
21. Avi Avigdor
22. Henry Ji
23. Andreas Herrmann

Reviewed by ScreenIT

High-Titer Neutralizing Antibodies against the SARS-CoV-2 Delta Variant Induced by Alhydroxyquim-II-Adjuvanted Trimeric Spike Antigens

1. Claudio Counoupas
2. Paco Pino
3. Alberto O. Stella
4. Caroline Ashley
5. Hannah Lukeman
6. Nayan D. Bhattacharyya
7. Takuya Tada
8. Stephanie Anchisi
9. Charles Metayer
10. Jacopo Martinis
11. Anupriya Aggarwal
12. Belinda M. Dcosta
13. Warwick J. Britton
14. Joeri Kint
15. Maria J. Wurm
16. Nathaniel R. Landau
17. Megan Steain
18. Stuart G. Turville
19. Florian M. Wurm
20. Sunil A. David
21. James A. Triccas

Reviewed by ScreenIT

Development of an in vitro model for animal species susceptibility to SARS-CoV-2 replication based on expression of ACE2 and TMPRSS2 in avian cells

1. Darrell R. Kapczynski
2. Ryan Sweeney
3. Erica Spackman
4. Mary Pantin-Jackwood
5. David L. Suarez

Reviewed by ScreenIT

Mutations that adapt SARS-CoV-2 to mustelid hosts do not increase fitness in the human airway

1. Jie Zhou
2. Thomas P. Peacock
3. Jonathan C. Brown
4. Daniel H. Goldhill
5. Ahmed M.E. Elrefaey
6. Rebekah Penrice-Randal
7. Vanessa M. Cowton
8. Giuditta De Lorenzo
9. Wilhelm Furnon
10. William T. Harvey
11. Ruthiran Kugathasan
12. Rebecca Frise
13. Laury Baillon
14. Ria Lassaunière
15. Nazia Thakur
16. Giulia Gallo
17. Hannah Goldswain
18. I’ah Donovan-Banfield
19. Xiaofeng Dong
20. Nadine P. Randle
21. Fiachra Sweeney
22. Martha C. Glynn
23. Jessica L. Quantrill
24. Paul F. McKay
25. Arvind H. Patel
26. Massimo Palmarini
27. Julian A. Hiscox
28. Dalan Bailey
29. Wendy S. Barclay

Reviewed by Rapid Reviews Infectious Diseases, ScreenIT

SARS-CoV-2 hijacks p38β/MAPK11 to promote virus replication

1. Christina A. Higgins
2. Benjamin E. Nilsson-Payant
3. Andrew P. Kurland
4. Chengjin Ye
5. Tomer Yaron
6. Jared L. Johnson
7. Boris Bonaventure
8. Prithy Adhikary
9. Ilona Golynker
10. Maryline Panis
11. Oded Danziger
12. Brad R. Rosenberg
13. Lewis C. Cantley
14. Luis Martinez-Sobrido
15. Benjamin R. tenOever
16. Jeffrey R. Johnson

Reviewed by ScreenIT

Integration of RT-LAMP and Microfluidic Technology for Detection of SARS-CoV-2 in Wastewater as an Advanced Point-of-Care Platform

1. Ahmed Donia
2. Muhammad Furqan Shahid
3. Sammer-ul Hassan
4. Ramla Shahid
5. Aftab Ahmad
6. Aneela Javed
7. Muhammad Nawaz
8. Tahir Yaqub
9. Habib Bokhari

Reviewed by ScreenIT

Pseudotyped Bat Coronavirus RaTG13 is efficiently neutralised by convalescent sera from SARS-CoV-2 infected patients

1. Diego Cantoni
2. Martin Mayora-Neto
3. Nazia Thakur
4. Ahmed M. E. Elrefaey
5. Joseph Newman
6. Sneha Vishwanath
7. Angalee Nadesalingam
8. Andrew Chan
9. Peter Smith
10. Javier Castillo-Olivares
11. Helen Baxendale
12. Bryan Charleston
13. Jonathan Heeney
14. Dalan Bailey
15. Nigel Temperton

Reviewed by ScreenIT

N-dihydrogalactochitosan reduces mortality in a lethal mouse model of SARS-CoV-2

1. Christopher M. Weiss
2. Hongwei Liu
3. Erin E. Ball
4. Ashley R. Hoover
5. Talia S. Wong
6. Chun Fung Wong
7. Samuel Lam
8. Tomas Hode
9. M. Kevin Keel
10. Richard M. Levenson
11. Wei R. Chen
12. Lark L. Coffey

Reviewed by ScreenIT