Microbiology

Elicitation of potent SARS-CoV-2 neutralizing antibody responses through immunization with a versatile adenovirus-inspired multimerization platform

1. Christopher Chevillard
2. Axelle Amen
3. Solène Besson
4. Dalil Hannani
5. Isabelle Bally
6. Valentin Dettling
7. Evelyne Gout
8. Christophe J. Moreau
9. Marlyse Buisson
10. Salomé Gallet
11. Daphna Fenel
12. Emilie Vassal-Stermann
13. Guy Schoehn
14. Pascal Poignard
15. Marie-Claire Dagher
16. Pascal Fender

Reviewed by ScreenIT

Adaptation and compensation in a bacterial gene regulatory network evolving under antibiotic selection

1. Vishwa Patel
2. Nishad Matange

• Curated by eLife

  Evaluation Summary:

  This paper investigates the evolutionary path of Escherichia coli resistance to the antibiotic trimethoprim. The authors show that adaptive mutations that accumulate early are often not in the drug target itself, but rather mutations that lead to transcriptional up-regulation of the drug target. Higher-level resistance can then evolve due to the addition of mutations in the drug target; however, at lower drug concentrations, cells are more likely to accumulate mutations that reverse the fitness defect associated with the initially acquired mutations. Overall, this study shows that regulatory mutations can play a major role in the evolution of antibiotic resistance in bacterial populations, and that the evolutionary path is influenced by the level of drug exposure.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewers #1 , #2, and #3 agreed to share their names with the authors.)

Reviewed by eLife

Heat efficiently inactivates coronaviruses inside vehicles

1. Krithika P. Karthigeyan
2. Chloe Flanigan
3. Denis Jacob Machado
4. Alper A. Kiziltas
5. Daniel A. Janies
6. Jay Chen
7. David Cooke
8. Marcia V. Lee
9. Linda J. Saif
10. Sonny Henegar
11. Jeff Jahnes
12. Deborah F. Mielewski
13. Jesse J. Kwiek

Reviewed by ScreenIT

Cigarette smoke preferentially induces full length ACE2 exposure in primary human airway cells but does not alter susceptibility to SARS-CoV-2 infection

1. Linsey M Porter
2. Wenrui Guo
3. Thomas WM Crozier
4. Edward JD Greenwood
5. Brian Ortmann
6. Daniel Kottmann
7. James A Nathan
8. Ravindra Mahadeva
9. Paul J Lehner
10. Frank McCaughan

Reviewed by ScreenIT

Artemisia annua L. hot-water extracts show potent activity in vitro against Covid-19 variants including delta

1. M.S. Nair
2. Y. Huang
3. D.A. Fidock
4. M.J. Towler
5. P.J. Weathers

Reviewed by ScreenIT

SARS-CoV-2 expresses a microRNA-like small RNA able to selectively repress host genes

1. Paulina Pawlica
2. Therese A. Yario
3. Sylvia White
4. Jianhui Wang
5. Walter N. Moss
6. Pei Hui
7. Joseph M. Vinetz
8. Joan A. Steitz

Reviewed by ScreenIT

An anti-SARS-CoV-2 non-neutralizing antibody with Fc-effector function defines a new NTD epitope and delays neuroinvasion and death in K18-hACE2 mice

1. Guillaume Beaudoin-Bussières
2. Yaozong Chen
3. Irfan Ullah
4. Jérémie Prévost
5. William D. Tolbert
6. Kelly Symmes
7. Shilei Ding
8. Mehdi Benlarbi
9. Shang Yu Gong
10. Alexandra Tauzin
11. Romain Gasser
12. Debashree Chatterjee
13. Dani Vézina
14. Guillaume Goyette
15. Jonathan Richard
16. Fei Zhou
17. Leonidas Stamatatos
18. Andrew T. McGuire
19. Hughes Charest
20. Michel Roger
21. Edwin Pozharski
22. Priti Kumar
23. Walther Mothes
24. Pradeep D. Uchil
25. Marzena Pazgier
26. Andrés Finzi

Reviewed by ScreenIT

A morphological transformation in respiratory syncytial virus leads to enhanced complement deposition

1. Jessica P Kuppan
2. Margaret D Mitrovich
3. Michael D Vahey

• Curated by eLife

  Evaluation Summary:

  This paper is of broad interest to virologists, immunologists, and structural biologists as it provides new insights into how the biophysical properties of viruses shape the development of immune responses. Overall, the data presented support key claims of the paper, and the strengths and limitations of the approach are properly acknowledged. However, the working model and its implications could be clarified and extended.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Neutralization of the SARS-CoV-2 Mu Variant by Convalescent and Vaccine Serum

1. Keiya Uriu
2. Izumi Kimura
3. Kotaro Shirakawa
4. Akifumi Takaori-Kondo
5. Taka-aki Nakada
6. Atsushi Kaneda
7. So Nakagawa
8. Kei Sato

Reviewed by ScreenIT

SARS-CoV-2 originated from SARS-CoV-1-related Bat-CoVs through Pan-CoVs rather than from SARS-CoV-2-related Bat-CoVs

1. Perumal Arumugam Desingu
2. K. Nagarajan

Reviewed by ScreenIT