Microbiology

Divergent acyl carrier protein decouples mitochondrial Fe-S cluster biogenesis from fatty acid synthesis in malaria parasites

1. Seyi Falekun
2. Jaime Sepulveda
3. Yasaman Jami-Alahmadi
4. Hahnbeom Park
5. James A Wohlschlegel
6. Paul A Sigala

• Curated by eLife

  Evaluation Summary:

  This study defines the role of a divergent mitochondrial-localized isoform of a FASII acyl carrier protein (mACP) in the malaria parasite, Plasmodium falciparum. In contrast to the situation in other eukaryotes, mACP is not involved in fatty acid biosynthesis, but is primarily involved in stabilizing proteins involved in mitochondrial Fe-S complex formation. Analysis of mACP function in these protists indicates that ACP acquired a role in Fe-S complex formation early in eukaryotic evolution and highlights additional components of the Plasmodium respiratory chain that are important for viability.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Spike-Independent Infection of Human Coronavirus 229E in Bat Cells

1. Marcus G. Mah
2. Martin Linster
3. Dolyce H. W. Low
4. Yan Zhuang
5. Jayanthi Jayakumar
6. Firdaus Samsudin
7. Foong Ying Wong
8. Peter J. Bond
9. Ian H. Mendenhall
10. Yvonne C. F. Su
11. Gavin J. D. Smith

Reviewed by ScreenIT

Deactivation of SARS-CoV-2 surrogate porcine epidemic diarrhea virus with electron beam irradiation under the cold chain transportation condition

1. Yan Liu
2. Yang Shao
3. Lu Wang
4. Weilai Lu
5. Shihua Li
6. Diandou Xu
7. Yu Vincent Fu

Reviewed by ScreenIT

Apixaban, an orally available anticoagulant, inhibits SARS-CoV-2 replication by targeting its major protease in a non-competitive way

1. Otávio Augusto Chaves
2. Carolina Q. Sacramento
3. Natalia Fintelman-Rodrigues
4. Jairo Ramos Temerozo
5. Filipe Pereira-Dutra
6. Daniella M. Mizurini
7. Robson Q. Monteiro
8. Leonardo Vazquez
9. Patricia T. Bozza
10. Hugo Caire Castro-Faria-Neto
11. Thiago Moreno L. Souza

Reviewed by ScreenIT

Aminoglycoside Antibiotics Inhibit Phage Infection by Blocking an Early Step of the Infection Cycle

1. Larissa Kever
2. Aël Hardy
3. Tom Luthe
4. Max Hünnefeld
5. Cornelia Gätgens
6. Lars Milke
7. Johanna Wiechert
8. Johannes Wittmann
9. Cristina Moraru
10. Jan Marienhagen
11. Julia Frunzke

• Curated by eLife

  Evaluation Summary:

  Earlier work demonstrated the inhibition of phage infection by aminoglycosides in Mycobacteria. Following up on this prior work, the authors demonstrate that the acetylated form of apramycin retains its anti-phage activity while blocking its antibacterial activity. The authors observed that MgCl abrogated the anti-phage effects of aminoglycosides and that MgCl can inhibit aminoglycoside uptake leading to the conclusion that antibiotic uptake was likely important for the anti-phage effects. Consistent with this conclusion, the initial stages of phage infection (adsorption and DNA injection) were not impacted by the antibiotics.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Inducible CRISPR activation screen for interferon-stimulated genes identifies OAS1 as a SARS-CoV-2 restriction factor

1. Oded Danziger
2. Roosheel S. Patel
3. Emma J. DeGrace
4. Mikaela R. Rosen
5. Brad R. Rosenberg

Reviewed by ScreenIT

A novel B.1.1.523 SARS-CoV-2 variant that combines many spike mutations linked to immune evasion with current variants of concern

1. Brian M.J.W. van der Veer
2. Jozef Dingemans
3. Lieke B. van Alphen
4. Christian J.P.A. Hoebe
5. Paul H.M. Savelkoul

Reviewed by ScreenIT

Personal care formulations demonstrate virucidal efficacy against multiple SARS-CoV-2 variants of concern: implications for hand hygiene

1. Sayandip Mukherjee
2. Carol K. Vincent
3. Harshinie W. Jayasekera
4. Ashish Shrikant Yekhe

Reviewed by ScreenIT

High-throughput super-resolution analysis of influenza virus pleomorphism reveals insights into viral spatial organization

1. Andrew McMahon
2. Rebecca Andrews
3. Danielle Groves
4. Sohail V. Ghani
5. Thorben Cordes
6. Achillefs N. Kapanidis
7. Nicole C. Robb

Reviewed by ScreenIT

Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodies

1. Myriam Scherer
2. Clarissa Read
3. Gregor Neusser
4. Christine Kranz
5. Anna K Kuderna
6. Regina Müller
7. Florian Full
8. Sonja Wörz
9. Anna Reichel
10. Eva-Maria Schilling
11. Paul Walther
12. Thomas Stamminger

• Curated by eLife

  Evaluation Summary:

  This work has potential broad interest to both virologists and cell biologists interested in the regulatory functions of promyelocytic leukaemia nuclear bodies (PML-NBs). The authors use a combination of imaging techniques to identify PML, the principal scaffolding protein of PML-NBs, to form a variety of different structures in response to viral infection, immune stimulation, and DNA damage. Thestudy identifies PML to restrict the replication of human cytomegalovirus (HCMV) at multiple stages of infection through the formation of alternate PML-scaffold assemblies.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife