Microbiology

Discovery of coordinately regulated pathways that provide innate protection against interbacterial antagonism

1. See-Yeun Ting
2. Kaitlyn D LaCourse
3. Hannah E Ledvina
4. Rutan Zhang
5. Matthew C Radey
6. Hemantha D Kulasekara
7. Rahul Somavanshi
8. Savannah K Bertolli
9. Larry A Gallagher
10. Jennifer Kim
11. Kelsi M Penewit
12. Stephen J Salipante
13. Libin Xu
14. S Brook Peterson
15. Joseph D Mougous

• Curated by eLife

  Evaluation Summary:

  Using a genome wide screen, the authors identify novel pathways that protect Pseudomonas aeruginosa against Type-6 secretion-mediated by other bacterial species. Importantly these pathways each protect against specific effectors. As an example, the authors further show that one factor Arc3B protects against phospholipase activity by antagonizing the production of lysophospholipids induced by phospholipase-type effectors. These findings uncover potentially conserved and unsuspected bacterial-defense pathways.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife, PREreview

Distinct neutralizing kinetics and magnitudes elicited by different SARS-CoV-2 variant spikes

1. Yang Liu
2. Jianying Liu
3. Jing Zou
4. Ping Ren
5. Scott C. Weaver
6. Xuping Xie
7. Pei-Yong Shi

Reviewed by ScreenIT

Characterization of SARS-CoV-2 variants B.1.617.1 (Kappa), B.1.617.2 (Delta) and B.1.618 on cell entry, host range, and sensitivity to convalescent plasma and ACE2 decoy receptor

1. Wenlin Ren
2. Xiaohui Ju
3. Mingli Gong
4. Jun Lan
5. Yanying Yu
6. Quanxin Long
7. Yu Zhang
8. Jin Zhong
9. Guocai Zhong
10. Xinquan Wang
11. Ailong Huang
12. Rong Zhang
13. Qiang Ding

Reviewed by ScreenIT

Epistasis at the SARS-CoV-2 RBD Interface and the Propitiously Boring Implications for Vaccine Escape

1. Nash D. Rochman
2. Guilhem Faure
3. Yuri I. Wolf
4. Peter L. Freddolino
5. Feng Zhang
6. Eugene V. Koonin

Reviewed by Rapid Reviews Infectious Diseases, ScreenIT

Gut bacterial aggregates as living gels

1. Brandon H Schlomann
2. Raghuveer Parthasarathy

• Curated by eLife

  Evaluation Summary:

  This manuscript provides an innovative merging of biophysical models with imaging data to explain the physical structure of microbial communities in the gut of zebrafish. Using imaging data to examine cluster sizes for eight different bacterial strains in the larval zebrafish gut, the authors report a common family of size distributions and show that these distributions arise naturally from a simple biophysical model of aggregation that tends to condense the system to a single massive cluster, reminiscent of gel formation observed in non-living systems. Within-host microbial dynamics represent an area of tremendous interest, as the microbiome is increasingly recognized to play a role in host physiology. This work contributes to a new perspective by elucidating physical mechanisms driving spatial segregation of these communities, opening the door to future studies that incorporate traditional genomic and microbiological insight with the physical and mechanical dynamics of microbial communities in living hosts.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Genetic and Structural Analysis of SARS-CoV-2 Spike Protein for Universal Epitope Selection

1. Christopher Markosian
2. Daniela I. Staquicini
3. Prashant Dogra
4. Esteban Dodero-Rojas
5. Joseph H. Lubin
6. Fenny H.F. Tang
7. Tracey L. Smith
8. Vinícius G. Contessoto
9. Steven K. Libutti
10. Zhihui Wang
11. Vittorio Cristini
12. Sagar D. Khare
13. Paul C. Whitford
14. Stephen K. Burley
15. José N. Onuchic
16. Renata Pasqualini
17. Wadih Arap

Reviewed by ScreenIT

May a Strain Chlamydia Isolated From SARS Patient’s Autopsy Issues Inhibit the Proliferation of SARS-CoV? An Early Observation in Vitro

1. Xing Quan
2. Weifei Liu

Reviewed by ScreenIT

Protective activity of mRNA vaccines against ancestral and variant SARS-CoV-2 strains

1. Baoling Ying
2. Bradley Whitener
3. Laura A. VanBlargan
4. Ahmed O. Hassan
5. Swathi Shrihari
6. Chieh-Yu Liang
7. Courtney E. Karl
8. Samantha Mackin
9. Rita E. Chen
10. Natasha M. Kafai
11. Samuel H. Wilks
12. Derek J. Smith
13. Juan Manuel Carreño
14. Gagandeep Singh
15. Florian Krammer
16. Andrea Carfi
17. Sayda M. Elbashir
18. Darin K. Edwards
19. Larissa B. Thackray
20. Michael S. Diamond

Reviewed by ScreenIT

Effects of Spike Mutations in SARS-CoV-2 Variants of Concern on Human or Animal ACE2-Mediated Virus Entry and Neutralization

1. Yunjeong Kim
2. Natasha N. Gaudreault
3. David A. Meekins
4. Krishani D. Perera
5. Dashzeveg Bold
6. Jessie D. Trujillo
7. Igor Morozov
8. Chester D. McDowell
9. Kyeong-Ok Chang
10. Juergen A. Richt

Reviewed by ScreenIT

The B.1.427/1.429 (epsilon) SARS-CoV-2 variants are more virulent than ancestral B.1 (614G) in Syrian hamsters

1. Timothy Carroll
2. Douglas Fox
3. Neeltje van Doremalen
4. Erin Ball
5. Mary Kate Morris
6. Alicia Sotomayor-Gonzalez
7. Venice Servellita
8. Arjun Rustagi
9. Claude Kwe Yinda
10. Linda Fritts
11. Julia Rebecca Port
12. Zhong-Min Ma
13. Myndi G. Holbrook
14. Jonathan Schulz
15. Catherine A. Blish
16. Carl Hanson
17. Charles Y. Chiu
18. Vincent Munster
19. Sarah Stanley
20. Christopher J. Miller

Reviewed by ScreenIT