Microbiology

Clinically observed deletions in SARS‐CoV‐2 Nsp1 affect its stability and ability to inhibit translation

1. Pravin Kumar
2. Erin Schexnaydre
3. Karim Rafie
4. Tatsuaki Kurata
5. Ilya Terenin
6. Vasili Hauryliuk
7. Lars‐Anders Carlson

Reviewed by ScreenIT

Stenoparib, an inhibitor of cellular poly (ADP-ribose) polymerases (PARPs), blocks in vitro replication of SARS-CoV-2 variants

1. Katherine E. Zarn
2. Sierra A. Jaramillo
3. Anthony R. Zapata
4. Nathan E. Stone
5. Ashley N. Jones
6. Haley E. Nunnally
7. Erik W. Settles
8. Ken Ng
9. Paul S. Keim
10. Steen Knudsen
11. Patricia M. Nuijten
12. Aloys S. L. Tijsma
13. Christopher T. French

Reviewed by ScreenIT

mRNA vaccines induce rapid antibody responses in mice

1. Makda S. Gebre
2. Susanne Rauch
3. Nicole Roth
4. Janina Gergen
5. Jingyou Yu
6. Xiaowen Liu
7. Andrew C. Cole
8. Stefan O. Mueller
9. Benjamin Petsch
10. Dan H. Barouch

Reviewed by ScreenIT

Coronavirus RNA Synthesis Takes Place within Membrane-Bound Sites

1. Nicole Doyle
2. Jennifer Simpson
3. Philippa C. Hawes
4. Helena J. Maier

Reviewed by ScreenIT

The P681H mutation in the Spike glycoprotein confers Type I interferon resistance in the SARS-CoV-2 alpha (B.1.1.7) variant

1. Maria Jose Lista
2. Helena Winstone
3. Harry D Wilson
4. Adam Dyer
5. Suzanne Pickering
6. Rui Pedro Galao
7. Giuditta De Lorenzo
8. Vanessa M. Cowton
9. Wilhelm Furnon
10. Nicolas Suarez
11. Richard Orton
12. Massimo Palmarini
13. Arvind H. Patel
14. Luke Snell
15. Gaia Nebbia
16. Chad Swanson
17. Stuart J D Neil

Reviewed by ScreenIT

Variations in Cell Surface ACE2 Levels Alter Direct Binding of SARS-CoV-2 Spike Protein and Viral Infectivity: Implications for Measuring Spike Protein Interactions with Animal ACE2 Orthologs

1. Soheila Kazemi
2. Alberto Domingo López-Muñoz
3. Jaroslav Hollý
4. Ling Jin
5. Jonathan W. Yewdell
6. Brian P. Dolan

Reviewed by ScreenIT

Toxoplasma bradyzoites exhibit physiological plasticity of calcium and energy stores controlling motility and egress

1. Yong Fu
2. Kevin M Brown
3. Nathaniel G Jones
4. Silvia NJ Moreno
5. L David Sibley

• Curated by eLife

  Evaluation Summary:

  The cyst-forming stages of Toxoplasma gondii that perpetuate chonic infections in more than a quarter of the world's human population exist in a metabolically quiescent state. This study provides evidence that metabolic quiescence in bradyzoite cysts is associated with a profound dampening of calcium signalling, including uptake and release from internal stores, which is reversed following bradyzoite egress and exposure to exogenous calcium and carbon sources.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Updated vaccine protects from infection with SARS-CoV-2 variants, prevents transmission and is immunogenic against Omicron in hamsters

1. Sapna Sharma
2. Thomas Vercruysse
3. Lorena Sanchez-Felipe
4. Winnie Kerstens
5. Madina Rasulova
6. Rana Abdelnabi
7. Caroline S Foo
8. Viktor Lemmens
9. Dominique Van Looveren
10. Piet Maes
11. Guy Baele
12. Birgit Weynand
13. Philippe Lemey
14. Johan Neyts
15. Hendrik Jan Thibaut
16. Kai Dallmeier

Reviewed by ScreenIT

Inhibition of SAR S-CoV-2 infection and replication by lactoferrin, MUC1 and α-lactalbumin identified in human breastmilk

1. Xinyuan Lai
2. Yanying Yu
3. Wei Xian
4. Fei Ye
5. Xiaohui Ju
6. Yuqian Luo
7. Huijun Dong
8. Yihua Zhou
9. Wenjie Tan
10. Hui Zhuang
11. Tong Li
12. Xiaoyun Liu
13. Qiang Ding
14. Kuanhui Xiang

Reviewed by ScreenIT

Ribonucleotide reductase, a novel drug target for gonorrhea

1. Jana Narasimhan
2. Suzanne Letinski
3. Stephen P Jung
4. Aleksey Gerasyuto
5. Jiashi Wang
6. Michael Arnold
7. Guangming Chen
8. Jean Hedrick
9. Melissa Dumble
10. Kanchana Ravichandran
11. Talya Levitz
12. Chang Cui
13. Catherine L Drennan
14. JoAnne Stubbe
15. Gary Karp
16. Arthur Branstrom

• Curated by eLife

  Evaluation Summary:

  This paper is of interest to biochemists and those focused on development of novel antibiotics. The authors present two small molecules that specifically target the essential ribonucleotide reductase of the causative agent of gonorrhea, with biochemical, biophysical, and biological data supporting the efficacy of these molecules both in vitro and in mouse models. Overall, this is a comprehensive study providing some interesting insights to guide the development of new therapies for malaria.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife