Microbiology

Aminoglycoside antibiotics inhibit phage infection by blocking an early step of the phage infection cycle

1. Larissa Kever
2. Aël Hardy
3. Tom Luthe
4. Max Hünnefeld
5. Cornelia Gätgens
6. Lars Milke
7. Johanna Wiechert
8. Johannes Wittmann
9. Cristina Moraru
10. Jan Marienhagen
11. Julia Frunzke

• Curated by eLife

  Evaluation Summary:

  Earlier work demonstrated the inhibition of phage infection by aminoglycosides in Mycobacteria. Following up on this prior work, the authors demonstrate that the acetylated form of apramycin retains its anti-phage activity while blocking its antibacterial activity. The authors observed that MgCl abrogated the anti-phage effects of aminoglycosides and that MgCl can inhibit aminoglycoside uptake leading to the conclusion that antibiotic uptake was likely important for the anti-phage effects. Consistent with this conclusion, the initial stages of phage infection (adsorption and DNA injection) were not impacted by the antibiotics.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Inducible CRISPR activation screen for interferon-stimulated genes identifies OAS1 as a SARS-CoV-2 restriction factor

1. Oded Danziger
2. Roosheel S. Patel
3. Emma J. DeGrace
4. Mikaela R. Rosen
5. Brad R. Rosenberg

Reviewed by ScreenIT

A novel B.1.1.523 SARS-CoV-2 variant that combines many spike mutations linked to immune evasion with current variants of concern

1. Brian M.J.W. van der Veer
2. Jozef Dingemans
3. Lieke B. van Alphen
4. Christian J.P.A. Hoebe
5. Paul H.M. Savelkoul

Reviewed by ScreenIT

Personal care formulations demonstrate virucidal efficacy against multiple SARS-CoV-2 variants of concern: implications for hand hygiene

1. Sayandip Mukherjee
2. Carol K. Vincent
3. Harshinie W. Jayasekera
4. Ashish Shrikant Yekhe

Reviewed by ScreenIT

High-throughput super-resolution analysis of influenza virus pleomorphism reveals insights into viral spatial organization

1. Andrew McMahon
2. Rebecca Andrews
3. Danielle Groves
4. Sohail V. Ghani
5. Thorben Cordes
6. Achillefs N. Kapanidis
7. Nicole C. Robb

Reviewed by ScreenIT

Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodies

1. Myriam Scherer
2. Clarissa Read
3. Gregor Neusser
4. Christine Kranz
5. Anna K Kuderna
6. Regina Müller
7. Florian Full
8. Sonja Wörz
9. Anna Reichel
10. Eva-Maria Schilling
11. Paul Walther
12. Thomas Stamminger

• Curated by eLife

  Evaluation Summary:

  This work has potential broad interest to both virologists and cell biologists interested in the regulatory functions of promyelocytic leukaemia nuclear bodies (PML-NBs). The authors use a combination of imaging techniques to identify PML, the principal scaffolding protein of PML-NBs, to form a variety of different structures in response to viral infection, immune stimulation, and DNA damage. Thestudy identifies PML to restrict the replication of human cytomegalovirus (HCMV) at multiple stages of infection through the formation of alternate PML-scaffold assemblies.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

ZRC3308 Monoclonal Antibody Cocktail Shows Protective Efficacy in Syrian Hamsters against SARS-CoV-2 Infection

1. Pragya D. Yadav
2. Sanjeev Kumar Mendiratta
3. Sreelekshmy Mohandas
4. Arun K. Singh
5. Priya Abraham
6. Anita Shete
7. Sanjay Bandyopadhyay
8. Sanjay Kumar
9. Aashini Parikh
10. Pankaj Kalita
11. Vibhuti Sharma
12. Hardik Pandya
13. Chirag G. Patel
14. Mihir Patel
15. Swagat Soni
16. Suresh Giri
17. Mukul Jain

Reviewed by ScreenIT

Genetically diverse mouse models of SARS-CoV-2 infection reproduce clinical variation in type I interferon and cytokine responses in COVID-19

1. Shelly J. Robertson
2. Olivia Bedard
3. Kristin L. McNally
4. Carl Shaia
5. Chad S. Clancy
6. Matthew Lewis
7. Rebecca M. Broeckel
8. Abhilash I. Chiramel
9. Jeffrey G. Shannon
10. Gail L. Sturdevant
11. Rebecca Rosenke
12. Sarah L. Anzick
13. Elvira Forte
14. Christoph Preuss
15. Candice N. Baker
16. Jeffrey M. Harder
17. Catherine Brunton
18. Steven Munger
19. Daniel P. Bruno
20. Justin B. Lack
21. Jacqueline M. Leung
22. Amirhossein Shamsaddini
23. Paul Gardina
24. Daniel E. Sturdevant
25. Jian Sun
26. Craig Martens
27. Steven M. Holland
28. Nadia A. Rosenthal
29. Sonja M. Best

Reviewed by ScreenIT

A novel hamster model of SARS-CoV-2 respiratory infection using a pseudotyped virus

1. Hiroshi Yamada
2. So-Ichiro Sasaki
3. Hideki Tani
4. Mayu Somekawa
5. Hitoshi Kawasuji
6. Yumiko Saga
7. Yoshihiro Yoshida
8. Yoshihiro Yamamoto
9. Yoshihiro Hayakawa
10. Yoshitomo Morinaga

Reviewed by ScreenIT

Virophages and retrotransposons colonize the genomes of a heterotrophic flagellate

1. Thomas Hackl
2. Sarah Duponchel
3. Karina Barenhoff
4. Alexa Weinmann
5. Matthias G Fischer

• Curated by eLife

  Evaluation Summary:

  Approaching the search of novel viruses while in an endogenized stage, rather than as free virions, the study by Hackl et al. reveals a large diversity of complete and fragmented virophage genomes - termed EMALEs- scattered throughout the genomes of four strains of the marine protist Cafeteria. Given that the activation of the integrated virophage mavirus during infection by the giant virus, CroV, has been shown to have a protective effect on the Cafeteria population, this study provides a tantalizing window into the traces of virophage-giant virus¬-protist interactions in the marine environment. Given the enormous diversity of virophages and giant viruses that have been found in metagenomes with no known hosts, this study is a step towards deciphering the biology of these viruses.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife