Microbiology

Neutralizing human monoclonal antibodies that target the PcrV component of the type III secretion system of Pseudomonas aeruginosa act through distinct mechanisms

1. Jean-Mathieu Desveaux
2. Eric Faudry
3. Carlos Contreras-Martel
4. François Cretin
5. Leonardo Sebastian Dergan-Dylon
6. Axelle Amen
7. Isabelle Bally
8. Victor Tardivy-Casemajor
9. Fabien Chenavier
10. Delphine Fouquenet
11. Yvan Caspar
12. Ina Attree
13. Andrea Dessen
14. Pascal Poignard

• Curated by eLife

  eLife Assessment

  This useful work identifies new monoclonal antibodies produced by cystic fibrosis patients against Pseudomonas aeruginosa type three secretion system. The evidence supporting authors' claim is solid. Nonetheless, the manuscript may benefit from a more in depth description of what the authors learned from their structure-based analyses of antibodies targeting PcrV.

Reviewed by eLife

Non-destructive label-free automated identification of bacterial colonies at the species level directly on agar media using digital holography and convolutional neural network algorithms

1. Prisca Perlemoine
2. Jordan Belissard
3. Bruno Burtschell
4. Nassim Halli
5. Luc Martin
6. Camille Brunet
7. Maxime Gougis
8. Patrick Schiavone
9. Yvan Caspar

Reviewed by Arcadia Science

Provision of Preferred Nutrients to Macrophages Enables Salmonella to Replicate Intracellularly Without Relying on Type III Secretion Systems

1. Francisco-Javier Garcia-Rodriguez
2. Camila Valenzuela
3. Joaquin Bernal-Bayard
4. Pedro Escoll

Reviewed by Review Commons

Resource landscape shapes the composition and stability of the human vaginal microbiota

1. Tsukushi Kamiya
2. Mircea T. Sofonea
3. Michael France
4. Nicolas Tessandier
5. Ignacio G Bravo
6. Carmen Lia Murall
7. Jacques Ravel
8. Samuel Alizon

Reviewed by Peer Community in Ecology

Applying 3D correlative structured illumination microscopy and X-ray tomography to characterise herpes simplex virus-1 morphogenesis

1. Kamal L Nahas
2. Viv Connor
3. Kaveesha J Wijesinghe
4. Henry G Barrow
5. Ian M Dobbie
6. Maria Harkiolaki
7. Stephen C Graham
8. Colin M Crump

• Curated by eLife

  eLife Assessment

  This landmark manuscript comprehensively examines the roles of nine structural proteins in herpes simplex virus 1 (HSV-1) assembly and nuclear egress. By integrating cryo-light microscopy and soft X-ray tomography, the study presents an innovative approach to investigating viral assembly within cells. The research is thoroughly executed, yielding exceptional data that explain previously unknown functions expected to bear widespread influence. This work is of broad interest to virologists, cellular biologists, and structural biologists, offering a robust, contextually rich methodology for studying large protein complex assembly within the cellular environment, serving as an excellent starting point for high-resolution techniques.

Reviewed by eLife

The increase in cell volume and nuclear number of the koji-fungus Aspergillus oryzae contributes to its high enzyme productivity

1. Ayaka Itani
2. Haruto Motomura
3. Ken Oda
4. Hideyuki Yamashita
5. Kanae Sakai
6. Ken-ichi Kusumoto
7. Shinsuke Shigeto
8. Takehiko Ichikawa
9. Hosain Mohammad Mubarak
10. Takeshi Fukuma
11. Takuya Katayama
12. Jun-ichi Maruyama
13. Shunsuke Masuo
14. Naoki Takaya
15. Norio Takeshita

• Curated by eLife

  eLife Assessment

  The ratio of nuclei to cell volume is a well-controlled parameter in eukaryotic cells. This study now reports important findings that expand our understanding of the regulatory relationship between cell size and number of nuclei. The evidence supporting the conclusions is convincing obtained by applying appropriate and validated methodology in line with current state-of-the-art. The paper will be of broad interest for cell biologists and fungal biotechnologists seeking to understand mechanisms determining cell size and number of nuclei and why this knowledge might also be of importance for the production of enzymes and thus production strains not only of Aspergillus oryzae but also other industrially used fungi.

Reviewed by eLife

High-throughput discovery of emerging antifungal resistance in crop pathogens

1. Guido Puccetti
2. Daniel Flückiger
3. Dominique Edel
4. Camille Delude
5. Sabina Moser Tralamazza
6. Thomas Badet
7. Gabriel Scalliet
8. Daniel Croll

Reviewed by PREreview

IFIT1 is rapidly evolving and exhibits disparate antiviral activities across 11 mammalian orders

1. Matthew B McDougal
2. Ian N Boys
3. Anthony M De Maria
4. Emi Nakahara
5. John W Schoggins

• Curated by eLife

  eLife Assessment

  This important report describes the changing antiviral activity of IFIT1 across mammals and in response to distinct viruses, likely as a result of past arms races. One of the main strengths of the manuscript is the breadth of mammalian IFIT1 orthologs and viruses that were tested, as well as the thoroughness of the positive selection analysis. Overall the evidence is convincing, and the discussion conveys well the limitations due to physical interactions with other IFITs that are not accounted for.

Reviewed by eLife

The capsule and genetic background, rather than specific individual loci, strongly influence in vitro pneumococcal growth kinetics

1. Chrispin Chaguza
2. Daan W Arends
3. Stephanie W Lo
4. Indri Hapsari Putri
5. Anna York
6. John A Lees
7. Anne L Wyllie
8. Daniel M Weinberger
9. Stephen D Bentley
10. Marien I de Jonge
11. Amelieke JH Cremers

• Curated by eLife

  eLife Assessment

  This is an important study that examines the impact of Streptococcus pneumoniae genetics on its in vitro growth kinetics, aiming to identify potential targets for vaccines and therapeutics. The study identified significant variations in growth characteristics among capsular serotypes and lineages, linked to phylogeny and high heritability, but genome-wide association studies did not reveal specific genomic loci associated with growth features independent of the genetic background. The evidence supporting these findings is convincing.

Reviewed by eLife

RBMX2 links Mycobacterium bovis infection to epithelial–mesenchymal transition and lung cancer progression

1. Chao Wang
2. Yongchong Peng
3. Hongxin Yang
4. Yanzhu Jiang
5. Abdul Karim Khalid
6. Kailun Zhang
7. Shengsong Xie
8. Luiz Bermudez
9. Yong Yang
10. Lei Zhang
11. Huanchun Chen
12. Aizhen Guo
13. Yingyu Chen

• Curated by eLife

  eLife Assessment

  The identification of RBMX2 as a novel regulator linking mycobacterial infection to Epithelial-Mesenchymal Transition and cancer progression are fundamental findings that advance our understanding of a major research question about the link between infectious and non-infectious diseases, microbiology and oncology. It does so by introducing RBMX2 as a novel host factor, a potential therapeutic target and biomarker for both TB and lung cancer. The evidence provided is convincing because it is appropriate and the validated multi-omics methodologies used are in line with the current state of the art. This study will be of interest to scientists working in the fields of drug discovery, microbiology and oncology.

Reviewed by eLife