Microbiology

Integrator complex subunit 12 knockout overcomes a transcriptional block to HIV latency reversal

1. Carley N Gray
2. Manickam Ashokkumar
3. Derek H Janssens
4. Jennifer L Kirchherr
5. Brigitte Allard
6. Emily Hsieh
7. Terry L Hafer
8. Nancie M Archin
9. Edward P Browne
10. Michael Emerman

• Curated by eLife

  eLife Assessment

  Using multiple techniques previously validated by the authors, this study identified INTS12, a component of the Integrator complex involved in 3' processing of small nuclear RNAs U1 and U2, as a factor promoting HIV-1 latency. The work is valuable, based on a sound strategy for screening targets to activate HIV latency and the solid mechanistic insights it provides on INTS12 repression of transcriptional elongation. Future studies are needed to explore INTS12 as a drug target against HIV/AIDS.

Reviewed by eLife

First worldwide detection of bla _IMP-15 in Stenotrophomonas maltophilia isolated from a patient in Lebanon.

1. Ziad C. Jabbour
2. Jose Rita Gerges
3. Hadi M. Hussein
4. Sara B. Barada
5. Lama Hamadeh
6. Rami Mahfouz
7. Zeina Kanafani
8. Ghassan M Matar
9. Antoine Abou Fayad

Reviewed by PREreview

Control of pili synthesis and putrescine homeostasis in Escherichia coli

1. Iti Mehta
2. Jacob B Hogins
3. Sydney R Hall
4. Gabrielle Vragel
5. Sankalya Ambagaspitiye
6. Philippe E Zimmern
7. Larry Reitzer

• Curated by eLife

  eLife Assessment

  This valuable study presents an interesting analysis of the role of the polyamine precursor putrescine in the pili-dependent surface motility of a laboratory strain of Escherichia coli. The overall data convincingly demonstrate a role in this case. This study presents interesting findings for those studying uropathogenic bacteria, and those studying bacterial polyamine function.

Reviewed by eLife

Immunopeptidomics informs discovery and delivery of Mycobacterium tuberculosis MHC-II antigens for vaccine design

1. Owen Leddy
2. Paul Ogongo
3. Julia Huffaker
4. Mingyu Gan
5. Ryan Milligan
6. Sheikh Mahmud
7. Yuko Yuki
8. Kidist Bobosha
9. Liya Wassie
10. Mary Carrington
11. Qingyun Liu
12. Joel D. Ernst
13. Forest M. White
14. Bryan D. Bryson

Reviewed by Rapid Reviews Infectious Diseases

Characterisation and comparison of semen microbiota and bacterial load in men with infertility, recurrent miscarriage, or proven fertility

1. Shahriar Mowla
2. Linda Farahani
3. Tharu Tharakan
4. Rhianna Davies
5. Goncalo DS Correia
6. Yun S Lee
7. Samit Kundu
8. Shirin Khanjani
9. Emad Sindi
10. Raj Rai
11. Lesley Regan
12. Dalia Khalifa
13. Ralf Henkel
14. Suks Minhas
15. Waljit S Dhillo
16. Jara Ben Nagi
17. Phillip Bennett
18. David A MacIntyre
19. Channa N Jayasena

• Curated by eLife

  eLife Assessment

  This valuable study reports a potential connection between the seminal microbiome and sperm quality/male fertility. The data are generally convincing. This study will be of interest to clinicians and biomedical researchers who work on microbiome and male fertility.

Reviewed by eLife

Structural basis for collagen recognition by the Streptococcus pyogenes M3 protein and its involvement in biofilm

1. Marta Wojnowska
2. Takeaki Wajima
3. Tamas Yelland
4. Hannes Ludewig
5. Robert M Hagan
6. Grant Watt
7. Samir W Hamaia
8. Dominique Bihan
9. Jean-Daniel Malcor
10. Arkadiusz Bonna
11. Helena Bergsten
12. Mattias Svensson
13. Oddvar Oppegaard
14. Steinar Skrede
15. Per Arnell
16. Ole Hyldegaard
17. Richard W Farndale
18. Anna Norrby-Teglund
19. Ulrich Schwarz-Linek

• Curated by eLife

  eLife Assessment

  In this paper, the authors report important structural and functional findings on the interaction of how the group A streptococci (GAS) M3 protein (expressed on GAS strains emm3, which are associated with invasive disease) binds to human collagens. They demonstrate an unusual T-shaped structure within the N-terminal hypervariable region of M3 protein that can bind two copies of collagen triple helix in parallel. These solid data advance understanding of how GAS M3 interacts with human collagen, information relevant to understanding and developing treatments for GAS infection. A major limitation of the work is the lack of mutational work to test if the T-shaped structure is necessary for binding collagen.

Reviewed by eLife

Whole Genome Sequencing Reveals Enterobacter hormaechei as a Key Bloodstream Pathogen in Six Tertiary Care Hospitals in Southwestern Nigeria

1. Faith I. Oni
2. Ayorinde O. Afolayan
3. Anderson O. Oaikhena
4. Erkison Ewomazino Odih
5. Odion O. Ikhimiukor
6. Veronica O. Ogunleye
7. Aaron O. Aboderin
8. Olatunde F. Olabisi
9. Adewale A. Amupitan
10. Abayomi Fadeyi
11. Rasaki A. Raheem
12. Bashirat A. Olanipekun
13. Charles J. Elikwu
14. Oluwadamilola A. Sadare
15. Phillip O. Oshun
16. Oyinlola O. Oduyebo
17. Folashade Ojo
18. Abolaji T. Adeyemo
19. Ifeanyi E Mba
20. Abiodun Egwuenu
21. Tochi Okwor
22. Anthony Underwood
23. Silvia Argimón
24. Chikwe Ihekweazu
25. David M. Aanensen
26. Iruka N. Okeke

Reviewed by Rapid Reviews Infectious Diseases

Discovery of antibiotics in the archaeome using deep learning

1. Marcelo D. T. Torres
2. Fangping Wan
3. Cesar de la Fuente-Nunez

Reviewed by Arcadia Science

A functional interleukin-4 homolog is encoded in the genome of infectious laryngotracheitis virus: unveiling a novel virulence factor

1. Jeremy D. Volkening
2. Stephen J. Spatz
3. Maricarmen García
4. Teresa A. Ross
5. Daniel A. Maekawa
6. Kenneth S. Rosenthal
7. Ana C. Zamora
8. April Skipper
9. Julia Blakey
10. Roshan Paudel

Reviewed by Arcadia Science

Morphology-dependent entry kinetics and spread of influenza A virus

1. Sarah Peterl
2. Carmen M. Lahr
3. Carl N. Schneider
4. Janis Meyer
5. Xenia Podlipensky
6. Vera Lechner
7. Maria Villiou
8. Larissa Eis
9. Steffen Klein
10. Charlotta Funaya
11. Elisabetta Ada Cavalcanti-Adam
12. Frederik Graw
13. Christine Selhuber-Unkel
14. Karl Rohr
15. Petr Chlanda

Reviewed by Review Commons, PREreview