Microbiology

In vitro reconstitution reveals membrane clustering and RNA recruitment by the enteroviral AAA+ ATPase 2C

1. Kasturika Shankar
2. Marie N. Sorin
3. Himanshu Sharma
4. Oskar Skoglund
5. Selma Dahmane
6. Josy Ter Beek
7. Solomon Tesfalidet
8. Louise Nenzén
9. Lars-Anders Carlson

Reviewed by Review Commons

Global Release of Translational Repression Across Plasmodium’s Host-to-Vector Transmission Event

1. Kelly T. Rios
2. James P. McGee
3. Aswathy Sebastian
4. Robert L. Moritz
5. Marina Feric
6. Sabrina Absalon
7. Kristian E. Swearingen
8. Scott E. Lindner

Reviewed by Review Commons

Frequent intertrophic transmission of Wolbachia by parasitism but not predation

1. Zhi-Chao Yan
2. Lan-Da Qi
3. Han-Le Ji
4. Xiao-Xiang Wang
5. Xiao-Yue Hong
6. Yuan-Xi Li

• Curated by eLife

  eLife assessment

  Using experiments in the white fly, this manuscript provides evidence that the bacterial symbiont Wolbachia can be transmitted from parasitoid wasps to their insect hosts. Characterizing the transfer of Wolbachia between insect species is a valuable attempt to explain the widespread of this intracellular bacterium. This paper is incomplete as it does not furnish sufficient data to support several of its claims for which additional methods and data are necessary.

Reviewed by eLife

Targeting plasmid-encoded proteins that contain immunoglobulin-like domains to combat antimicrobial resistance

1. Alejandro Prieto
2. Luïsa Miró
3. Yago Margolles
4. Manuel Bernabeu
5. David Salguero
6. Susana Merino
7. Joan Tomas
8. Juan Alberto Corbera
9. Anna Perez-Bosque
10. Mario Huttener
11. Luis Ángel Fernández
12. Antonio Juarez

• Curated by eLife

  eLife assessment

  This important and novel study addresses the challenge of antimicrobial resistance by targeting plasmid proteins that interfere with plasmid transfer as a strategy to limit the spread of antibiotic-resistance genes. The evidence presented and the integration of two approaches to tackle antimicrobial resistance is convincing. This work will interest those working on plasmid transfer and antimicrobial resistance.

Reviewed by eLife

Potential pandemic risk of circulating swine H1N2 influenza viruses

1. Valerie Le Sage
2. Nicole C. Rockey
3. Kevin R. McCarthy
4. Andrea J. French
5. Meredith J. Shephard
6. Ryan McBride
7. Jennifer E. Jones
8. Sydney G. Walter
9. Joshua D. Doyle
10. Lingqing Xu
11. Dominique J. Barbeau
12. Shengyang Wang
13. Sheila A. Frizzell
14. Lora H. Rigatti
15. Michael M. Myerburg
16. James C. Paulson
17. Anita K. McElroy
18. Tavis K. Anderson
19. Amy L. Vincent Baker
20. Seema S. Lakdawala

Reviewed by PREreview

In-silico docking platform with serine protease inhibitor (SERPIN) structures identifies host cysteine protease targets with significance for SARS-CoV-2

1. Joaquín J Rodriguez Galvan
2. Maren de Vries
3. Shiraz Belblidia
4. Ashley Fisher
5. Rachel A Prescott
6. Keaton M Crosse
7. Walter F. Mangel
8. Ralf Duerr
9. Meike Dittmann

Reviewed by Review Commons

Divergent downstream biosynthetic pathways are supported by L-cysteine synthases of Mycobacterium tuberculosis

1. Mehak Zahoor Khan
2. Debbie M Hunt
3. Biplab Singha
4. Yogita Kapoor
5. Nitesh Kumar Singh
6. D V Sai Prasad
7. Sriram Dharmarajan
8. Divya Tej Sowpati
9. Luiz Pedro S de Carvalho
10. Vinay Kumar Nandicoori

• Curated by eLife

  eLife assessment

  Sulphur atoms derived from cysteine are thought to play significant roles in maintaining redox homeostasis in Mycobacterium tuberculosis, which encounters stresses associated with immune cell interactions. In this valuable manuscript, the authors provide solid evidence that the genes encoding cysteine biosynthetic enzymes (cysM and cysK2) are required to maintain full viability of M. tuberculosis under in vitro stress conditions, macrophage infections, and within the lung tissues of mice. The manuscript presents transcriptomic and metabolomic evidence to support the hypothesis that CysM and CysK2 play distinct roles in maintaining cysteine-derived metabolite pools under stress conditions. The work will be of interest to microbiologists in general.

Reviewed by eLife

Bacteria are a major determinant of Orsay virus transmission and infection in Caenorhabditis elegans

1. Brian G Vassallo
2. Noemie Scheidel
3. Sylvia E J Fischer
4. Dennis H Kim

• Curated by eLife

  eLife assessment

  Using a C. elegans/virus system, this important work demonstrates that viral susceptibility can be greatly altered by the bacterial food that C. elegans consumes. The work is rigorous with solid support for the conclusions: the authors show that quorum-sensing compounds play a role in reducing host susceptibility, and they perform control experiments to rule out nutrition and pathogenicity of the bacteria as the cause of impacts on viral susceptibility.

Reviewed by eLife

The target of rapamycin signaling pathway regulates vegetative development, aflatoxin biosynthesis, and pathogenicity in Aspergillus flavus

1. Guoqi Li
2. Xiaohong Cao
3. Elisabeth Tumukunde
4. Qianhua Zeng
5. Shihua Wang

• Curated by eLife

  eLife assessment

  This manuscript provides important information about the influence of TOR signaling pathway on development and aflatoxin production in the plant and human fungal pathogen Aspergillus flavus. Compared to an earlier version, the authors have addressed most of the concerns of the reviewers, including the convincing demonstration of the essential TOR pathway in this fungus by constructing a xylose promoter mutant strain.

Reviewed by eLife

Unraveling the gut microbiota of Tibetan chickens: insights into highland adaptation and ecological advantages

1. Tao Zeng
2. Yongqing Cao
3. Jianmei Yin
4. Peishi Feng
5. Yong Tian
6. Hanxue Sun
7. Tiantian Gu
8. Yibo Zong
9. Xueying Ma
10. Zelong Zhao
11. Li Chen
12. Wenwu Xu
13. Wei Han
14. Lizhi Lu

Reviewed by PREreview