Microbiology

Virological characteristics of the SARS-CoV-2 Omicron BA.2 spike

1. Daichi Yamasoba
2. Izumi Kimura
3. Hesham Nasser
4. Yuhei Morioka
5. Naganori Nao
6. Jumpei Ito
7. Keiya Uriu
8. Masumi Tsuda
9. Jiri Zahradnik
10. Kotaro Shirakawa
11. Rigel Suzuki
12. Mai Kishimoto
13. Yusuke Kosugi
14. Kouji Kobiyama
15. Teppei Hara
16. Mako Toyoda
17. Yuri L. Tanaka
18. Erika P. Butlertanaka
19. Ryo Shimizu
20. Hayato Ito
21. Lei Wang
22. Yoshitaka Oda
23. Yasuko Orba
24. Michihito Sasaki
25. Kayoko Nagata
26. Kumiko Yoshimatsu
27. Hiroyuki Asakura
28. Mami Nagashima
29. Kenji Sadamasu
30. Kazuhisa Yoshimura
31. Jin Kuramochi
32. Motoaki Seki
33. Ryoji Fujiki
34. Atsushi Kaneda
35. Tadanaga Shimada
36. Taka-aki Nakada
37. Seiichiro Sakao
38. Takuji Suzuki
39. Takamasa Ueno
40. Akifumi Takaori-Kondo
41. Ken J. Ishii
42. Gideon Schreiber
43. Hirofumi Sawa
44. Akatsuki Saito
45. Takashi Irie
46. Shinya Tanaka
47. Keita Matsuno
48. Takasuke Fukuhara
49. Terumasa Ikeda
50. Kei Sato

Reviewed by ScreenIT

S-217622, a SARS-CoV-2 main protease inhibitor, decreases viral load and ameliorates COVID-19 severity in hamsters

1. Michihito Sasaki
2. Koshiro Tabata
3. Mai Kishimoto
4. Yukari Itakura
5. Hiroko Kobayashi
6. Takuma Ariizumi
7. Kentaro Uemura
8. Shinsuke Toba
9. Shinji Kusakabe
10. Yuki Maruyama
11. Shun Iida
12. Noriko Nakajima
13. Tadaki Suzuki
14. Shinpei Yoshida
15. Haruaki Nobori
16. Takao Sanaki
17. Teruhisa Kato
18. Takao Shishido
19. William W. Hall
20. Yasuko Orba
21. Akihiko Sato
22. Hirofumi Sawa

Reviewed by ScreenIT

A live-attenuated SARS-CoV-2 vaccine candidate with accessory protein deletions

1. Yang Liu
2. Xianwen Zhang
3. Jianying Liu
4. Hongjie Xia
5. Jing Zou
6. Antonio E. Muruato
7. Sivakumar Periasamy
8. Chaitanya Kurhade
9. Jessica A. Plante
10. Nathen E. Bopp
11. Birte Kalveram
12. Alexander Bukreyev
13. Ping Ren
14. Tian Wang
15. Vineet D. Menachery
16. Kenneth S. Plante
17. Xuping Xie
18. Scott C. Weaver
19. Pei-Yong Shi

Reviewed by ScreenIT

SARS-CoV-2 Permissive glioblastoma cell line for high throughput antiviral screening

1. Emiel Vanhulle
2. Joren Stroobants
3. Becky Provinciael
4. Anita Camps
5. Sam Noppen
6. Piet Maes
7. Kurt Vermeire

Reviewed by ScreenIT

Malaria parasites utilize pyrophosphate to fuel an essential proton pump in the ring stage and the transition to trophozoite stage

1. Omobukola Solebo
2. Liqin Ling
3. Jing Zhou
4. Tian-Min Fu
5. Hangjun Ke

• Curated by eLife

  Evaluation Summary:

  This work will be of interest to researchers seeking new ways to target malaria parasites. The work provides insight into the energy requirements of parasites during the first day of their two-day life cycle, a period during which they are significantly resistant to a wide variety of antimalarial drugs, and identifies an essential enzyme that could be targeted in early-stage parasites. The study shows this protein is necessary for normal development and growth of parasites in red blood cells.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

In Vitro Selection of Remdesivir-Resistant SARS-CoV-2 Demonstrates High Barrier to Resistance

1. Liva Checkmahomed
2. Julie Carbonneau
3. Venice Du Pont
4. Nicholas C. Riola
5. Jason K. Perry
6. Jiani Li
7. Bastien Paré
8. Shawn M. Simpson
9. Martin A. Smith
10. Danielle P. Porter
11. Guy Boivin

Reviewed by ScreenIT

Remdesivir and GS-441524 Retain Antiviral Activity against Delta, Omicron, and Other Emergent SARS-CoV-2 Variants

1. Jared Pitts
2. Jiani Li
3. Jason K. Perry
4. Venice Du Pont
5. Nicholas Riola
6. Lauren Rodriguez
7. Xianghan Lu
8. Chaitanya Kurhade
9. Xuping Xie
10. Gregory Camus
11. Savrina Manhas
12. Ross Martin
13. Pei-Yong Shi
14. Tomas Cihlar
15. Danielle P. Porter
16. Hongmei Mo
17. Evguenia Maiorova
18. John P. Bilello

Reviewed by ScreenIT

Dual Inhibition of Cathepsin L and 3CL-Pro by GC-376 Constrains SARS Cov2 Infection Including Omicron Variant

1. Prabhakaran Kumar
2. Kiira M Ratia
3. Justin M Richner
4. Gregory R J Thatcher
5. Rashmi Kadam
6. Sandra P Smieszek
7. Bartlomiej P Przychodzen
8. Vuk Koprivica
9. Gunther Birznieks
10. Mihael H Polymeropoulos
11. Bellur S Prabhakar

Reviewed by ScreenIT

Host casein kinase 1-mediated phosphorylation modulates phase separation of a rhabdovirus phosphoprotein and virus infection

1. Xiao-Dong Fang
2. Qiang Gao
3. Ying Zang
4. Ji-Hui Qiao
5. Dong-Min Gao
6. Wen-Ya Xu
7. Ying Wang
8. Dawei Li
9. Xian-Bing Wang

• Curated by eLife

  Evaluation Summary:

  Recently liquid-liquid phase separation (LLPS) has emerged as a mechanism by which membraneless compartments are formed inside the cells to compartmentalize biomolecules. In this paper, the authors show that the P protein from a plant-infecting negative sense RNA virus undergoes LLPS to promote virus replication. The host casein kinase 1 phosphorylates P protein and inhibits phase separation and viral replication. This paper will be of interest to virologists and researchers who study LLPS.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Noisy metabolism can promote microbial cross-feeding

1. Jaime G Lopez
2. Ned S Wingreen

• Curated by eLife

  Evaluation Summary:

  This paper will be of broad interest to microbiologists interested in gene expression noise and/or metabolic interactions in microbial communities. It provides a novel hypothesis that complements existing theoretical frameworks. The hypothesis is well supported by data from a mathematical model, and it its predictions could be tested experimentally in future work.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife