Microbiology

Ivermectin Does Not Protect against SARS-CoV-2 Infection in the Syrian Hamster Model

1. Caroline S. Foo
2. Rana Abdelnabi
3. Laura Vangeel
4. Steven De Jonghe
5. Dirk Jochmans
6. Birgit Weynand
7. Johan Neyts

Reviewed by ScreenIT

Molnupiravir (MK-4482) is efficacious against Omicron and other SARS-CoV-2 variants in the Syrian hamster COVID-19 model

1. Kyle Rosenke
2. Atsushi Okumura
3. Matthew C. Lewis
4. Friederike Feldmann
5. Kimberly Meade-White
6. W. Forrest Bohler
7. Amanda Griffin
8. Rebecca Rosenke
9. Carl Shaia
10. Michael A. Jarvis
11. Heinz Feldmann

Reviewed by ScreenIT

Characterization of various remdesivir-resistant mutations of SARS-CoV-2 by mathematical modeling and molecular dynamics simulation

1. Shiho Torii
2. Kwang Su Kim
3. Jun Koseki
4. Rigel Suzuki
5. Shoya Iwanami
6. Yasuhisa Fujita
7. Yong Dam Jeong
8. Yoshiharu Matsuura
9. Teppei Shimamura
10. Shingo Iwami
11. Takasuke Fukuhara

Reviewed by ScreenIT

Serological screening in wild ruminants in Germany, 2021/2022: No evidence of SARS‐CoV‐2, bluetongue virus or pestivirus spread but high seroprevalences against Schmallenberg virus

1. Kerstin Wernike
2. Luisa Fischer
3. Mark Holsteg
4. Andrea Aebischer
5. Anja Petrov
6. Katharina Marquart
7. Ulrich Schotte
8. Jacob Schön
9. Donata Hoffmann
10. Silke Hechinger
11. Antonie Neubauer‐Juric
12. Julia Blicke
13. Thomas C. Mettenleiter
14. Martin Beer

Reviewed by ScreenIT

A Single Dose of the Deactivated Rabies-Virus Vectored COVID-19 Vaccine, CORAVAX, Is Highly Efficacious and Alleviates Lung Inflammation in the Hamster Model

1. Drishya Kurup
2. Christoph Wirblich
3. Leila Zabihi Diba
4. Rachael Lambert
5. Megan Watson
6. Noor Shaikh
7. Holly Ramage
8. Charalambos Solomides
9. Matthias J. Schnell

Reviewed by ScreenIT

Identification and differential usage of a host metalloproteinase entry pathway by SARS-CoV-2 Delta and Omicron

1. Mehdi Benlarbi
2. Geneviève Laroche
3. Corby Fink
4. Kathy Fu
5. Rory P. Mulloy
6. Alexandra Phan
7. Ardeshir Ariana
8. Corina M. Stewart
9. Jérémie Prévost
10. Guillaume Beaudoin-Bussières
11. Redaet Daniel
12. Yuxia Bo
13. Omar El Ferri
14. Julien Yockell-Lelièvre
15. William L. Stanford
16. Patrick M. Giguère
17. Samira Mubareka
18. Andrés Finzi
19. Gregory A. Dekaban
20. Jimmy D. Dikeakos
21. Marceline Côté

Reviewed by ScreenIT

Integration host factor regulates colonization factors in the bee gut symbiont Frischella perrara

1. Konstantin Schmidt
2. Gonçalo Santos-Matos
3. Stefan Leopold-Messer
4. Yassine El Chazli
5. Olivier Emery
6. Théodora Steiner
7. Joern Piel
8. Philipp Engel

• Curated by eLife

  Evaluation Summary:

  The gut of honeybees is colonized with stable community of bacterial symbionts. The molecular mechanisms involved in this stable associations are poorly understood. Here Schmidt et. al. focused on the identification of bacterial factors required for the colonization of the bee gut symbiont Frischella perrara. They identified a major regulator involved in controlling the expression of multiple colonization factors in F. perrara which include factors that are either required for the induction of a distinct scab phenotype upon gut colonization, or are involved in promoting colonization through adhesin or the production of secondary metabolites. The work is of potential interest for researchers in the field of host-microbe interactions and symbiosis, and for the study of bee health. The valuable integration of different techniques used in this study and rigor of the experiments lead to solid data and justified conclusions.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Critical role for isoprenoids in apicoplast biogenesis by malaria parasites

1. Megan Okada
2. Krithika Rajaram
3. Russell P Swift
4. Amanda Mixon
5. John Alan Maschek
6. Sean T Prigge
7. Paul A Sigala

• Curated by eLife

  Evaluation Summary:

  This is an excellent, innovative and high quality study that reveals an essential role for isoprenoids within the Plasmodium apicoplast, and demonstrates a likely polyprenol synthase that is required for apicoplast biogenesis. This is an important finding for understanding apicoplast and isoprenoid biology in general, and is significant because synthesis of isoprenoids appears to be the only essential role for the apicoplast in asexual intraerythrocytic stages.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Noise in a Metabolic Pathway Leads to Persister Formation in Mycobacterium tuberculosis

1. Jeffrey Quigley
2. Kim Lewis

• Curated by eLife

  Evaluation Summary:

  This manuscript is of interest to readers in the field of microbiology and antimicrobial resistance. It reports that genetically identical antibiotic-sensitive bacteria can escape killing upon exposure to an otherwise lethal concentration of antibiotics because they have a low ATP level. The authors further attempt to demonstrate that variation in the level of expression of some genes in energy-generating metabolic pathways allows for a wide range of ATP levels among the cells in a population, thus generating a subpopulation with low ATP levels that can survive antibiotic exposure.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Refolding of lid subdomain of SARS-CoV-2 nsp14 upon nsp10 interaction releases exonuclease activity

1. Anna Czarna
2. Jacek Plewka
3. Leanid Kresik
4. Alex Matsuda
5. Abdulkarim Karim
6. Colin Robinson
7. Sean O’Byrne
8. Fraser Cunningham
9. Irene Georgiou
10. Piotr Wilk
11. Magdalena Pachota
12. Grzegorz Popowicz
13. Paul Graham Wyatt
14. Grzegorz Dubin
15. Krzysztof Pyrć

Reviewed by ScreenIT