Microbiology

Identification and differential usage of a host metalloproteinase entry pathway by SARS-CoV-2 Delta and Omicron

1. Mehdi Benlarbi
2. Geneviève Laroche
3. Corby Fink
4. Kathy Fu
5. Rory P. Mulloy
6. Alexandra Phan
7. Ardeshir Ariana
8. Corina M. Stewart
9. Jérémie Prévost
10. Guillaume Beaudoin-Bussières
11. Redaet Daniel
12. Yuxia Bo
13. Omar El Ferri
14. Julien Yockell-Lelièvre
15. William L. Stanford
16. Patrick M. Giguère
17. Samira Mubareka
18. Andrés Finzi
19. Gregory A. Dekaban
20. Jimmy D. Dikeakos
21. Marceline Côté

Reviewed by ScreenIT

Integration host factor regulates colonization factors in the bee gut symbiont Frischella perrara

1. Konstantin Schmidt
2. Gonçalo Santos-Matos
3. Stefan Leopold-Messer
4. Yassine El Chazli
5. Olivier Emery
6. Théodora Steiner
7. Joern Piel
8. Philipp Engel

• Curated by eLife

  Evaluation Summary:

  The gut of honeybees is colonized with stable community of bacterial symbionts. The molecular mechanisms involved in this stable associations are poorly understood. Here Schmidt et. al. focused on the identification of bacterial factors required for the colonization of the bee gut symbiont Frischella perrara. They identified a major regulator involved in controlling the expression of multiple colonization factors in F. perrara which include factors that are either required for the induction of a distinct scab phenotype upon gut colonization, or are involved in promoting colonization through adhesin or the production of secondary metabolites. The work is of potential interest for researchers in the field of host-microbe interactions and symbiosis, and for the study of bee health. The valuable integration of different techniques used in this study and rigor of the experiments lead to solid data and justified conclusions.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Critical role for isoprenoids in apicoplast biogenesis by malaria parasites

1. Megan Okada
2. Krithika Rajaram
3. Russell P Swift
4. Amanda Mixon
5. John Alan Maschek
6. Sean T Prigge
7. Paul A Sigala

• Curated by eLife

  Evaluation Summary:

  This is an excellent, innovative and high quality study that reveals an essential role for isoprenoids within the Plasmodium apicoplast, and demonstrates a likely polyprenol synthase that is required for apicoplast biogenesis. This is an important finding for understanding apicoplast and isoprenoid biology in general, and is significant because synthesis of isoprenoids appears to be the only essential role for the apicoplast in asexual intraerythrocytic stages.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Noise in a Metabolic Pathway Leads to Persister Formation in Mycobacterium tuberculosis

1. Jeffrey Quigley
2. Kim Lewis

• Curated by eLife

  Evaluation Summary:

  This manuscript is of interest to readers in the field of microbiology and antimicrobial resistance. It reports that genetically identical antibiotic-sensitive bacteria can escape killing upon exposure to an otherwise lethal concentration of antibiotics because they have a low ATP level. The authors further attempt to demonstrate that variation in the level of expression of some genes in energy-generating metabolic pathways allows for a wide range of ATP levels among the cells in a population, thus generating a subpopulation with low ATP levels that can survive antibiotic exposure.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Refolding of lid subdomain of SARS-CoV-2 nsp14 upon nsp10 interaction releases exonuclease activity

1. Anna Czarna
2. Jacek Plewka
3. Leanid Kresik
4. Alex Matsuda
5. Abdulkarim Karim
6. Colin Robinson
7. Sean O’Byrne
8. Fraser Cunningham
9. Irene Georgiou
10. Piotr Wilk
11. Magdalena Pachota
12. Grzegorz Popowicz
13. Paul Graham Wyatt
14. Grzegorz Dubin
15. Krzysztof Pyrć

Reviewed by ScreenIT

Low Density Lipoprotein Receptor-Related Protein 1 (LRP1) as an auxiliary host factor for RNA viruses including SARS-CoV-2

1. Stephanie Devignot
2. Tim Wai Sha
3. Thomas R. Burkard
4. Patrick Schmerer
5. Astrid Hagelkruys
6. Ali Mirazimi
7. Ulrich Elling
8. Josef M. Penninger
9. Friedemann Weber

Reviewed by ScreenIT

Metagenomic analysis reveals the abundance and diversity of opportunistic fungal pathogens in the nasopharyngeal tract of COVID-19 patients

1. M. Nazmul Hoque
2. M. Shaminur Rahman
3. Md. Murshed Hasan Sarkar
4. Md Ahashan Habib
5. M. Anwar Hossain
6. M. Salim Khan
7. Tofazzal Islam

Reviewed by ScreenIT

SARS-CoV-2 has not emerged in roe, red or fallow deer in Germany or Austria during the COVID 19 pandemic

1. Andres Moreira-Soto
2. Christian Walzer
3. Gábor Á. Czirják
4. Martin Richter
5. Stephen F. Marino
6. Annika Posautz
7. Pau Rodo de Yebra
8. Gayle K. McEwen
9. Jan Felix Drexler
10. Alex D. Greenwood

Reviewed by ScreenIT

A natural broad-spectrum inhibitor of enveloped virus entry, effective against SARS-CoV-2 and Influenza A Virus in preclinical animal models

1. Rohan Narayan
2. Mansi Sharma
3. Rajesh Yadav
4. Abhijith Biji
5. Oyahida Khatun
6. Raju Rajmani
7. Pallavi Raj Sharma
8. Sharumathi Jeyasankar
9. Priya Rani
10. C. Durga Rao
11. Vijaya Satchidanandanam
12. Saumitra Das
13. Rachit Agarwal
14. Shashank Tripathi

Reviewed by Rapid Reviews Infectious Diseases, ScreenIT

An ACE2-blocking antibody confers broad neutralization and protection against Omicron and other SARS-CoV-2 variants

1. Wenjuan Du
2. Daniel L. Hurdiss
3. Dubravka Drabek
4. Anna Z. Mykytyn
5. Franziska K. Kaiser
6. Mariana González-Hernandez
7. Diego Muñoz-Santos
8. Mart M. Lamers
9. Rien van Haperen
10. Wentao Li
11. Ieva Drulyte
12. Chunyan Wang
13. Isabel Sola
14. Federico Armando
15. Georg Beythien
16. Malgorzata Ciurkiewicz
17. Wolfgang Baumgärtner
18. Kate Guilfoyle
19. Tony Smits
20. Joline van der Lee
21. Frank J.M. van Kuppeveld
22. Geert van Amerongen
23. Bart L. Haagmans
24. Luis Enjuanes
25. Albert D.M.E. Osterhaus
26. Frank Grosveld
27. Berend-Jan Bosch

Reviewed by ScreenIT