Pulmonary lesions following inoculation with the SARS-CoV-2 Omicron BA.1 (B.1.1.529) variant in Syrian golden hamsters

Article activity feed

1. Version published to 10.1080/22221751.2022.2095932

   Jul 17, 2022
2. 

   ScreenIT

   Mar 18, 2022

   SciScore for 10.1101/2022.03.15.484448: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	not detected.
   Sex as a biological variable	Animals and experimental setup: Female Syrian golden hamsters (Mesocricetus auratus; 6 weeks old; Janvier, France) were handled in an ABSL-3 biocontainment laboratory (Supplementary material).
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	Contamination: Cells were tested negative for mycoplasma.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Plaque assay: All throat swabs, nasal washes and tissue samples that were taken post mortem at 5 dpi were used for quantification of viral loads via a plaque assay on Calu-3 cells as described before [15] (Supplemental material).	Calu-3 suggested: …

   SciScore for 10.1101/2022.03.15.484448: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	not detected.
   Sex as a biological variable	Animals and experimental setup: Female Syrian golden hamsters (Mesocricetus auratus; 6 weeks old; Janvier, France) were handled in an ABSL-3 biocontainment laboratory (Supplementary material).
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	Contamination: Cells were tested negative for mycoplasma.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Plaque assay: All throat swabs, nasal washes and tissue samples that were taken post mortem at 5 dpi were used for quantification of viral loads via a plaque assay on Calu-3 cells as described before [15] (Supplemental material).	Calu-3 suggested: KCLB Cat# 30055, RRID:CVCL_0609)
   Software and Algorithms
   Sentences	Resources
   Plaque assay analysis was performed using ImageQuant TL 8.2 software (GE Healthcare).	ImageQuant suggested: (ImageQuant, RRID:SCR_014246)
   Statistical analysis: All statistical analyses were performed using GraphPad Prism 9 software (La Jolla, CA, USA).	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

   ---

   Results from OddPub: Thank you for sharing your data.

   ---

   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

   ---

   Results from TrialIdentifier: No clinical trial numbers were referenced.

   ---

   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

   ---

   Results from JetFighter: We did not find any issues relating to colormaps.

   ---

   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

   ---

   Results from scite Reference Check: We found no unreliable references.

   ---

   Read the original source
3. 

   Version published to 10.1101/2022.03.15.484448v1 on bioRxiv

   Mar 15, 2022