Microbiology

Microevolution of Trypanosoma cruzi reveals hybridization and clonal mechanisms driving rapid genome diversification

1. Gabriel Machado Matos
2. Michael D Lewis
3. Carlos Talavera-López
4. Matthew Yeo
5. Edmundo C Grisard
6. Louisa A Messenger
7. Michael A Miles
8. Björn Andersson

• Curated by eLife

  Evaluation Summary:

  In this paper the authors dissect the across-the-genome consequences of sexual recombination in Trypanosoma cruzi. It summarises an extensive piece of work, which includes a 5-year in vitro growing of parasites and relatively rigorous genome analyses. It will be of interest to microbiologists working on microbes with cryptic or parasexual modes of genetic exchange.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Impact of a human gut microbe on Vibrio cholerae host colonization through biofilm enhancement

1. Kelsey Barrasso
2. Denise Chac
3. Meti D Debela
4. Catherine Geigel
5. Anjali Steenhaut
6. Abigail Rivera Seda
7. Chelsea N Dunmire
8. Jason B Harris
9. Regina C Larocque
10. Firas S Midani
11. Firdausi Qadri
12. Jing Yan
13. Ana A Weil
14. Wai-Leung Ng

• Curated by eLife

  Evaluation Summary:

  In this work, the authors study the previously reported positive association between the presence of the gut bacterium Paracoccus aminovorans and Vibrio cholerae during infection. They describe and image dual species-biofilm formed in vitro as well as enhanced V. cholerae gut colonization in the presence of P. aminovorans in a neonatal mouse model. Collectively, the authors conclude that P. aminovorans enhances biofilm formation by Vc, which could explain the increased susceptibility of P. aminovorans-containing humans in cholera endemic areas.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Determining the Optimal SARS-CoV-2 mRNA Vaccine Dosing Interval for Maximum Immunogenicity

1. Michael Asamoah-Boaheng
2. David Goldfarb
3. Martin A Prusinkiewicz
4. Liam Golding
5. Mohammad E Karim
6. Vilte Barakauskas
7. Nechelle Wall
8. Agatha N Jassem
9. Ana Citlali Marquez
10. Chris MacDonald
11. Sheila F O’Brien
12. Pascal Lavoie
13. Brian Grunau

Reviewed by ScreenIT

Z-RNA and the flipside of the SARS Nsp13 helicase

1. Alan Herbert
2. Maria Poptsova

Reviewed by ScreenIT

Comparative infection and pathogenesis of SARS-CoV-2 Omicron and Delta variants in aged and young Syrian hamsters

1. Nadia Storm
2. Nicholas A. Crossland
3. Lindsay G. A. McKay
4. Anthony Griffiths

Reviewed by ScreenIT

A structural dynamic explanation for observed escape of SARS-CoV-2 BA.2 variant mutation S371L/F

1. Nathaniel L. Miller
2. Thomas Clark
3. Rahul Raman
4. Ram Sasisekharan

Reviewed by ScreenIT

A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates

1. Philip W Fowler
2. Carla Wright
3. Helen Spiers
4. Tingting Zhu
5. Elisabeth ML Baeten
6. Sarah W Hoosdally
7. Ana L Gibertoni Cruz
8. Aysha Roohi
9. Samaneh Kouchaki
10. Timothy M Walker
11. Timothy EA Peto
12. Grant Miller
13. Chris Lintott
14. David Clifton
15. Derrick W Crook
16. A Sarah Walker
17. The Zooniverse Volunteer Community
18. The CRyPTIC Consortium

• Curated by eLife

  Evaluation Summary:

  The authors evaluate a novel crowd-sourcing method to interpret minimum inhibitory concentrations of Mycobacterium tuberculosis, the causative agent of tuberculosis. To provide valuable test results without the need for available expert mycobacteriologists, the authors demonstrate that when presented appropriately, 11-17 interpretations by lay interpreters can provide reproducible results for most tuberculosis drugs. This analysis demonstrates that among those samples that can be reliably interpreted by automated detection software, lay interpretation provides a potential alternative means to provide a timely confirmatory read. The work will be of interest to bacteriologists and those with an interest in antimicrobial resistance.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Time-Dependent Increase in Susceptibility and Severity of Secondary Bacterial Infection during SARS-CoV-2 Infection

1. Amanda P. Smith
2. Evan P. Williams
3. Taylor R. Plunkett
4. Muneeswaran Selvaraj
5. Lindey C. Lane
6. Lillian Zalduondo
7. Yi Xue
8. Peter Vogel
9. Rudragouda Channappanavar
10. Colleen B. Jonsson
11. Amber M. Smith

Reviewed by ScreenIT

RK-33, a small molecule inhibitor of host RNA helicase DDX3, suppresses multiple variants of SARS-CoV-2

1. Farhad Vesuna
2. Ivan Akhrymuk
3. Amy Smith
4. Paul T. Winnard
5. Shih-Chao Lin
6. Lauren Panny
7. Robert Scharpf
8. Kylene Kehn-Hall
9. Venu Raman

Reviewed by ScreenIT

Omicron-specific mRNA vaccine elicits potent immune responses in mice, hamsters, and nonhuman primates

1. Yi Wu
2. Yanqiong Shen
3. Namei Wu
4. Xinghai Zhang
5. Shaohong Chen
6. Chang Yang
7. Junhui Zhou
8. Yan Wu
9. Da Chen
10. Li Wang
11. Chao Wang
12. Huajun Zhang
13. Ninuo Xia
14. Sandra Chiu
15. Yucai Wang

Reviewed by ScreenIT