Immunology

Intranasal Immunization with a Proteosome-Adjuvanted SARS-CoV2 Spike Protein-Based Vaccine is Immunogenic and Efficacious in Mice & Hamsters

1. Felicity C. Stark
2. Bassel Akache
3. Lise Deschatelets
4. Anh Tran
5. Matthew Stuible
6. Yves Durocher
7. Michael J. McCluskie
8. Gerard Agbayani
9. Renu Dudani
10. Blair A. Harrison
11. Tyler M. Renner
12. Shawn R. Makinen
13. Jegarubee Bavananthasivam
14. Diana Duque
15. Martin Gagne
16. Joseph Zimmermann
17. C. David Zarley
18. Terrence R. Cochrane
19. Martin Handfield

Reviewed by ScreenIT

Rapid Hypermutation B Cell Trajectory Recruits Previously Primed B Cells Upon Third SARS-Cov-2 mRNA Vaccination

1. Lisa Paschold
2. Bianca Klee
3. Cornelia Gottschick
4. Edith Willscher
5. Sophie Diexer
6. Christoph Schultheiß
7. Donjete Simnica
8. Daniel Sedding
9. Matthias Girndt
10. Michael Gekle
11. Rafael Mikolajczyk
12. Mascha Binder

Reviewed by ScreenIT

Protection of hamsters challenged with SARS-CoV-2 after two doses of MVC-COV1901 vaccine followed by a single intranasal booster with nanoemulsion adjuvanted S-2P vaccine

1. Yi-Jiun Lin
2. Meei-Yun Lin
3. Ya-Shan Chuang
4. Luke Tzu-Chi Liu
5. Tsun-Yung Kuo
6. Charles Chen
7. Shyamala Ganesan
8. Ali Fattom
9. Vira Bitko
10. Chia-En Lien

Reviewed by ScreenIT

An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques

1. Patrick J. Madden
2. Yanique Thomas
3. Robert V. Blair
4. Sadia Samer
5. Mark Doyle
6. Cecily C. Midkiff
7. Lara A. Doyle-Meyers
8. Mark E. Becker
9. Muhammad S. Arif
10. Michael D. McRaven
11. Lacy M. Simons
12. Ann M. Carias
13. Elena Martinelli
14. Ramon Lorenzo-Redondo
15. Judd F Hultquist
16. Francois J. Villinger
17. Ronald S. Veazey
18. Thomas J. Hope

Reviewed by ScreenIT

Discriminating cross-reactivity in polyclonal IgG1 responses against SARS-CoV-2 variants of concern

1. Danique M. H. van Rijswijck
2. Albert Bondt
3. Max Hoek
4. Karlijn van der Straten
5. Tom G. Caniels
6. Meliawati Poniman
7. Dirk Eggink
8. Chantal Reusken
9. Godelieve J. de Bree
10. Rogier W. Sanders
11. Marit J. van Gils
12. Albert J. R. Heck

Reviewed by ScreenIT

Neutrophil extracellular traps arm DC vaccination against NPM-mutant myeloproliferation

1. Claudio Tripodo
2. Barbara Bassani
3. Elena Jachetti
4. Valeria Cancila
5. Claudia Chiodoni
6. Paola Portararo
7. Laura Botti
8. Cesare Valenti
9. Milena Perrone
10. Maurilio Ponzoni
11. Patrizia Comoli
12. Mara Lecchi
13. Paolo Verderio
14. Antonio Curti
15. Mario P Colombo
16. Sabina Sangaletti

• Curated by eLife

  Evaluation Summary:

  The authors successfully demonstrated the immunogenic effects of a new dendritic cell-based vaccine on AML with NPM1 mutation. Using a transgenic mouse model that carries human NPM1 mutation and an elegant bone marrow chimera approach, they showed that the tumor growth is reduced by the vaccine. Furthermore, they provided evidence for increased CD8+ T cell presence and activity at the tumor site and increased anti-NPMc antibody levels in the serum. These findings are timely and novel and the new methods presented here would be of interest to a broad audience from immunology, inflammation and cancer fields.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

A high-throughput yeast display approach to profile pathogen proteomes for MHC-II binding

1. Brooke D Huisman
2. Zheng Dai
3. David K Gifford
4. Michael E Birnbaum

• Curated by eLife

  Evaluation Summary:

  The manuscript builds on previous work to design yeast display libraries representing full viral proteomes with overlapping 15-mer peptides binding to specific HLA-DR alleles, and therefore potentially immunogenic for CD4 T cell responses. The authors use SARS-CoV-2 and dengue viruses as proof of concept and identify a number of potentially immunogenic peptides not predicted by current algorithms. The methods are interesting and promising and will be of interest to a wide range of researchers in immunological and infectious disease studies.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife, ScreenIT

Modeling predicts mechanisms altered by mutations of the SARS-CoV-2 delta and omicron variants

1. Jason Pearson
2. Timothy Wessler
3. Alex Chen
4. Richard C. Boucher
5. Ronit Freeman
6. Samuel K. Lai
7. Raymond Pickles
8. M. Gregory Forest

Reviewed by ScreenIT

Pre-clinical testing of two serologically distinct chimpanzee-origin adenovirus vectors expressing spike of SARS-CoV-2

1. Mikhail Novikov
2. Mohadeseh Hasanpourghadi
3. Robert Ambrose
4. Arezki Chekaoui
5. Dakota Newman
6. Wynetta Giles-Davis
7. Zhiquan Xiang
8. Xiang Yang Zhou
9. Hildegund CJ Ertl

Reviewed by ScreenIT

A SARS-CoV-2 peptide vaccine which elicits T-cell responses in mice but does not protect against infection or disease

1. Victoria K. Baxter
2. Elizabeth J. Anderson
3. Sharon A. Taft-Benz
4. Kelly Olsen
5. Maria Sambade
6. Kaylee M. Gentry
7. Wolfgang Beck
8. Jason Garness
9. Allison Woods
10. Misha Fini
11. Brandon Carpenter
12. Christof C. Smith
13. Mark T. Heise
14. Benjamin Vincent
15. Alex Rubinsteyn

Reviewed by ScreenIT