Immunology

Inhibitory IL-10-producing CD4+ T cells are T-bet-dependent and facilitate cytomegalovirus persistence via coexpression of arginase-1

1. Mathew Clement
2. Kristin Ladell
3. Kelly L Miners
4. Morgan Marsden
5. Lucy Chapman
6. Anna Cardus Figueras
7. Jake Scott
8. Robert Andrews
9. Simon Clare
10. Valeriia V Kriukova
11. Ksenia R Lupyr
12. Olga V Britanova
13. David R Withers
14. Simon A Jones
15. Dmitriy M Chudakov
16. David A Price
17. Ian R Humphreys

• Curated by eLife

  Evaluation Summary:

  This manuscript analyses the inhibitory role of IL-10 producing regulatory T-cells in a mouse cytomegalovirus infection model. The authors report that IL-10 producing CD4+T-cells express genes of chronically activated Th1-cells, are clonally expanded and inhibit anti-viral T-cell responses via arginase, an enzyme that breaks down an essential amino acid for T-cell activation. The manuscript presents some novel and potentially important data; however, it requires the provision of additional experimental data and clarifications.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Epigenetic remodeling by vitamin C potentiates plasma cell differentiation

1. Heng-Yi Chen
2. Ana Almonte-Loya
3. Fang-Yun Lay
4. Michael Hsu
5. Eric Johnson
6. Edahí González-Avalos
7. Jieyun Yin
8. Richard S Bruno
9. Qin Ma
10. Hazem E Ghoneim
11. Daniel J Wozniak
12. Fiona E Harrison
13. Chan-Wang Jerry Lio

• Curated by eLife

  Evaluation Summary:

  This manuscript described a role of Vitamin C in promoting plasma cell differentiation by remodeling the epigenome via TET (Ten Eleven Translation) family proteins. Overall, most of experiments are properly executed, controlled and presented. This paper will be of interest to scientists in molecular immunologists, particularly those involved in of epigenetic mechanisms of B cell differentiation to plasma cells.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Cytotoxic CD4+ T cells driven by T-cell intrinsic IL-18R/MyD88 signaling predominantly infiltrate Trypanosoma cruzi-infected hearts

1. Carlos-Henrique D Barbosa
2. Fábio B Canto
3. Ariel Gomes
4. Layza M Brandao
5. Jéssica R Lima
6. Guilherme A Melo
7. Alessandra Granato
8. Eula GA Neves
9. Walderez O Dutra
10. Ana-Carolina Oliveira
11. Alberto Nóbrega
12. Maria Bellio

• Curated by eLife

  Evaluation Summary:

  This is an interesting study, conducted in mice, that demonstrates for the first time the presence of a large population of cytotoxic CD4+ T lymphocytes in infection with Trypanosoma cruzi, a relevant human pathogen. At present, the relevance of these cells in protective immunity engendered by the host remains unclear. Additional experiments are needed to characterize the functionality of these cytotoxic CD4 T cells vis-a-vis the canonical Th1 T cells. This paper can be of interest to scientists interested in immune responses to parasitic infections.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Transcriptome network analysis implicates CX3CR1-positive type 3 dendritic cells in non-infectious uveitis

1. Sanne Hiddingh
2. Aridaman Pandit
3. Fleurieke Verhagen
4. Rianne Rijken
5. Nila Hendrika Servaas
6. Rina CGK Wichers
7. Ninette H ten Dam-van Loon
8. Saskia M Imhof
9. Timothy RDJ Radstake
10. Joke H de Boer
11. Jonas JW Kuiper

• Curated by eLife

  Evaluation Summary:

  The authors provide evidence suggesting the gene expression profile of a specific subset of dendritic cells define features of specific forms of non-infectious uveitis. This work suggests specific pathways in these cells that may have mechanistic import in inflammatory eye disease. This manuscript is of interested to immunologists studying autoimmunity and ocular immunity. While the paper is largely descriptive, the data it presents should serve as a valuable resource for generating hypotheses about the pathogenesis of ocular autoimmune disease.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Analysis of allelic cross-reactivity of monoclonal IgG antibodies by a multiplexed reverse FluoroSpot assay

1. Henriette Hoffmann-Veltung
2. Nsoh Godwin Anabire
3. Michael Fokuo Ofori
4. Peter Janhmatz
5. Niklas Ahlborg
6. Lars Hviid
7. Maria del Pilar Quintana

• Curated by eLife

  Evaluation Summary:

  This manuscript describes a fluorospot-based assay as a model for a methodical, step-wise and rigorous approach - that combines multiple reagents in a complex system - to study the cross-reactivity of antibody to polymorphic antigens using the malaria vaccine candidate, VAR2CSA, as a model. The authors apply monoclonal antibodies and the corresponding B cells to validate their multiplexed assay before testing small number of samples from malaria exposed donors in a pilot application of the assay. The data support the conclusions. This information will attract the attention of immunologists and vaccinologists, who are primarily but not exclusively, involved in research on malaria.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Engineered natural killer cells impede the immunometabolic CD73-adenosine axis in solid tumors

1. Andrea M Chambers
2. Kyle B Lupo
3. Jiao Wang
4. Jingming Cao
5. Sagar Utturkar
6. Nadia Lanman
7. Victor Bernal-Crespo
8. Shadia Jalal
9. Sharon R Pine
10. Sandra Torregrosa-Allen
11. Bennett D Elzey
12. Sandro Matosevic

• Curated by eLife

  Evaluation Summary:

  The authors have engineered an anti-CD73 chimeric antigen receptor (CAR) that they express in NK cells to counteract tumors bearing CD73, which contributes to the generation of immunosuppressive adenosine in the tumor microenvironment. This is a promising approach for a new anti-cancer immunotherapy and will be of interest to oncologists and cancer immunologists. The CAR-bearing NK cells show slightly enhanced tumor killing in vitro, but preliminary data show more promising results in mice. This could be due to the CD73 CAR blocking catalytic activity in the tumor microenvironment more effectively than directly promoting cytotoxicity responses.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

The cIAP ubiquitin ligases sustain type 3 γδ T and innate lymphoid cells during aging to allow normal cutaneous and mucosal responses

1. John Rizk
2. Urs M. Mörbe
3. Rasmus Agerholm
4. Isabel Ulmert
5. Elisa Catafal Tardos
6. Darshana Kadekar
7. Maria V. Baglioni
8. Monica Torrellas Viñals
9. Vasileios Bekiaris

Reviewed by Review Commons

Neuron-associated macrophage proliferation in the sensory ganglia is associated with peripheral nerve injury-induced neuropathic pain involving CX3CR1 signaling

1. Rafaela M Guimarães
2. Conceição E Aníbal-Silva
3. Marcela Davoli-Ferreira
4. Francisco Isaac F Gomes
5. Atlante Mendes
6. Maria CM Cavallini
7. Miriam M Fonseca
8. Samara Damasceno
9. Larissa P Andrade
10. Marco Colonna
11. Cyril Rivat
12. Fernando Q Cunha
13. José C Alves-Filho
14. Thiago M Cunha

• Curated by eLife

  Evaluation Summary:

  Guimaraes et al address the origin of the macrophage increase in sensory ganglia after peripheral nerve injury. The authors show that there is no major influx by blood-derived monocytes into ganglia after injury and that resident macrophages proliferate, which is dependent on CX3CR1 signaling. Overall the work is clear and sound and should be of interest to immunologists and neurobiologists.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

The Nucleocapsid Protein Of SARS-CoV-2, Combined With ODN-39M, Is A Potential Component For An Intranasal Bivalent Pancorona Vaccine

1. Yadira Lobaina
2. Rong Chen
3. Edith Suzarte
4. Panchao Ai
5. Vivian Huerta
6. Alexis Musacchio
7. Ricardo Silva
8. Changyuan Tan
9. Alejandro Martin
10. Laura Lazo
11. Gerardo Guillén
12. Ke Yang
13. Yasser Perera
14. Lisset Hermida

Reviewed by ScreenIT

Antiviral immune responses, cellular metabolism and adhesion are differentially modulated by SARS-CoV-2 ORF7a or ORF7b

1. Tránsito García-García
2. Raúl Fernández-Rodríguez
3. Natalia Redondo
4. Ana de Lucas-Rius
5. Sara Zaldívar-López
6. Blanca Dies López-Ayllón
7. José M. Suárez-Cárdenas
8. Ángeles Jiménez-Marín
9. María Montoya
10. Juan J. Garrido

Reviewed by ScreenIT