A cocktail containing two synergetic antibodies broadly neutralizes SARS-CoV-2 and its variants including Omicron BA.1 and BA.2

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   ScreenIT

   Apr 28, 2022

   SciScore for 10.1101/2022.04.26.489529: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IACUC: Ethics statements: All procedures associated with the animal study were reviewed and approved by the Institutional Animal Care and Use Committee of the Institute of Wuhan Institute of Virology, Chinese Academy of Sciences, and were performed in an ABSL-3 facility (ethical approval number: WIVA45202104).
   Sex as a biological variable	Animals: Female Syrian hamsters (five to six weeks of age) were purchased from Wuhan Institute of Biological Products Co., Ltd.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   ALP-conjugated anti-human IgG antibody (ThermoFisher, A18808, 1:2000) was …

   SciScore for 10.1101/2022.04.26.489529: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IACUC: Ethics statements: All procedures associated with the animal study were reviewed and approved by the Institutional Animal Care and Use Committee of the Institute of Wuhan Institute of Virology, Chinese Academy of Sciences, and were performed in an ABSL-3 facility (ethical approval number: WIVA45202104).
   Sex as a biological variable	Animals: Female Syrian hamsters (five to six weeks of age) were purchased from Wuhan Institute of Biological Products Co., Ltd.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   ALP-conjugated anti-human IgG antibody (ThermoFisher, A18808, 1:2000) was used as the detection antibody at 37°C for 30 min .	anti-human IgG suggested: (Thermo Fisher Scientific Cat# A18808, RRID:AB_2535585) A18808 suggested: (ABclonal Cat# A18808, RRID:AB_2862443)
   The first antibody was allowed to associate for 600 s at 20 μg/mL, and the second protein (ACE2 (50 μg/mL) or a mixture of equal amounts of antibodies and ACE2) was allowed to associate for 300 s.	ACE2 suggested: None
   Animal protection experiments: To assess whether the 58G6 and 55A8 antibody cocktail induced synergistic effector function responses in vivo, hamsters were infected with 104 PFU of Omicron and treated with 58G6 (1500 μg), 55A8 (500 μg), or the 58G6 and 55A8 mixture (1000 μg of 58G6 and 300 μg of 55A8) at 1 h pre-infection and 24 and 48 h post-infection.	55A8 suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Viral stocks were prepared by propagation in Vero E6 cells (catalog no. ATCC® CRL-1586™) in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin and streptomycin (P/S).	Vero E6 suggested: None
   After 24 h, the luciferase activities of infected 293T/ACE2 cells were detected with the Bright-Luciferase Reporter Assay System (Beyotime, RG055M).	293T/ACE2 suggested: RRID:CVCL_YZ65)
   Software and Algorithms
   Sentences	Resources
   PRNT50 values were calculated in GraphPad Prism 9.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)
   Cryo-EM data processing: All dose-fractioned images were motion-corrected and dose-weighted with MotionCorr2 software45, and their contrast transfer functions (CTFs) were estimated by cryoSPARC patch CTF estimation46.	MotionCorr2 suggested: None cryoSPARC suggested: (cryoSPARC, RRID:SCR_016501)
   The 55A8 Fab model was first predicted using Phyre248 and then manually built in Coot 0.949 with the guidance of the cryo-EM electron density maps, and overall real-space refinements were performed using Phenix 1.1950.	Phenix suggested: (Phenix, RRID:SCR_014224)
   The 58G6 Fab model15 was manually built in Coot 0.9 with the guidance of the cryo-EM electron density maps, and overall real-space refinements were performed using Phenix 1.19.	Coot suggested: (Coot, RRID:SCR_014222)

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   Results from OddPub: Thank you for sharing your data.

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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   Version published to 10.1101/2022.04.26.489529v1 on bioRxiv

   Apr 26, 2022